DGT2_DROME
ID DGT2_DROME Reviewed; 231 AA.
AC Q9VKD6;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Augmin complex subunit dgt2 {ECO:0000305};
DE AltName: Full=Dim gamma-tubulin 2 {ECO:0000303|PubMed:17412918};
GN Name=dgt2 {ECO:0000303|PubMed:17412918, ECO:0000312|FlyBase:FBgn0032390};
GN ORFNames=CG16969 {ECO:0000312|FlyBase:FBgn0032390};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL48407.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL48407.1};
RC TISSUE=Testis {ECO:0000312|EMBL:AAL48407.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17412918; DOI=10.1126/science.1141314;
RA Goshima G., Wollman R., Goodwin S.S., Zhang N., Scholey J.M., Vale R.D.,
RA Stuurman N.;
RT "Genes required for mitotic spindle assembly in Drosophila S2 cells.";
RL Science 316:417-421(2007).
RN [5] {ECO:0000305}
RP FUNCTION, AND IDENTIFICATION IN THE AUGMIN COMPLEX.
RX PubMed=18443220; DOI=10.1083/jcb.200711053;
RA Goshima G., Mayer M., Zhang N., Stuurman N., Vale R.D.;
RT "Augmin: a protein complex required for centrosome-independent microtubule
RT generation within the spindle.";
RL J. Cell Biol. 181:421-429(2008).
RN [6] {ECO:0000305}
RP FUNCTION, INTERACTION WITH WAC, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RX PubMed=19289792; DOI=10.1083/jcb.200811102;
RA Meireles A.M., Fisher K.H., Colombie N., Wakefield J.G., Ohkura H.;
RT "Wac: a new Augmin subunit required for chromosome alignment but not for
RT acentrosomal microtubule assembly in female meiosis.";
RL J. Cell Biol. 184:777-784(2009).
RN [7] {ECO:0000305}
RP IDENTIFICATION IN THE AUGMIN COMPLEX, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=19369198; DOI=10.1073/pnas.0901587106;
RA Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C.,
RA Goshima G.;
RT "The augmin complex plays a critical role in spindle microtubule generation
RT for mitotic progression and cytokinesis in human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009).
RN [8] {ECO:0000305}
RP SUBCELLULAR LOCATION.
RX PubMed=23785300; DOI=10.1371/journal.pgen.1003562;
RA Colombie N., Gluszek A.A., Meireles A.M., Ohkura H.;
RT "Meiosis-specific stable binding of augmin to acentrosomal spindle poles
RT promotes biased microtubule assembly in oocytes.";
RL PLoS Genet. 9:E1003562-E1003562(2013).
CC -!- FUNCTION: As part of the augmin complex, plays a role in centrosome-
CC independent generation of spindle microtubules (PubMed:18443220). The
CC complex is required for mitotic spindle assembly through its
CC involvement in localizing gamma-tubulin to spindle microtubules
CC (PubMed:17412918). dgt2 binds to microtubules in vitro
CC (PubMed:19289792). {ECO:0000269|PubMed:17412918,
CC ECO:0000269|PubMed:18443220, ECO:0000269|PubMed:19289792}.
CC -!- SUBUNIT: Component of the augmin complex composed of dgt2, dgt3, dgt4,
CC dgt5, dgt6, msd1, msd5 and wac (PubMed:18443220, PubMed:19289792). The
CC complex interacts directly or indirectly with microtubules and is
CC required for centrosome-independent generation of spindle microtubules
CC (PubMed:18443220). dgt2 interacts directly with wac (via coiled coil)
CC (PubMed:19289792). {ECO:0000269|PubMed:18443220,
CC ECO:0000269|PubMed:19289792, ECO:0000269|PubMed:19369198}.
CC -!- INTERACTION:
CC Q9VKD6; Q9W0S8: wac; NbExp=3; IntAct=EBI-84322, EBI-180262;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:17412918, ECO:0000269|PubMed:19289792}. Cytoplasm,
CC cytoskeleton, spindle pole {ECO:0000269|PubMed:19289792,
CC ECO:0000269|PubMed:23785300}. Note=Stably associates with the polar
CC regions of some acentrosomal meiotic spindles in contrast to the
CC uniform staining observed over mitotic spindles in syncytial embryos
CC (PubMed:19289792, PubMed:23785300). {ECO:0000269|PubMed:19289792,
CC ECO:0000269|PubMed:23785300}.
CC -!- TISSUE SPECIFICITY: In adult females, detected only in the abdomen with
CC no expression in the head or thorax (at protein level).
CC {ECO:0000269|PubMed:19289792}.
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels in early embryos but at
CC low levels in larva, pupa and adult (at protein level).
CC {ECO:0000269|PubMed:19289792}.
CC -!- MISCELLANEOUS: The name 'dim gamma-tubulin 2' derives from the
CC decreased gamma-tubulin staining of the spindle pole seen following
CC RNAi-mediated knockdown of dgt2 in S2 cells.
CC {ECO:0000303|PubMed:17412918}.
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DR EMBL; AE014134; AAF53140.1; -; Genomic_DNA.
DR EMBL; AE014134; AHN54370.1; -; Genomic_DNA.
DR EMBL; AY070785; AAL48407.1; -; mRNA.
DR RefSeq; NP_001285856.1; NM_001298927.1.
DR RefSeq; NP_609531.1; NM_135687.5.
DR AlphaFoldDB; Q9VKD6; -.
DR SMR; Q9VKD6; -.
DR IntAct; Q9VKD6; 13.
DR STRING; 7227.FBpp0079869; -.
DR PaxDb; Q9VKD6; -.
DR PRIDE; Q9VKD6; -.
DR DNASU; 34610; -.
DR EnsemblMetazoa; FBtr0080285; FBpp0079869; FBgn0032390.
DR EnsemblMetazoa; FBtr0342580; FBpp0309535; FBgn0032390.
DR GeneID; 34610; -.
DR KEGG; dme:Dmel_CG16969; -.
DR UCSC; CG16969-RA; d. melanogaster.
DR CTD; 34610; -.
DR FlyBase; FBgn0032390; dgt2.
DR VEuPathDB; VectorBase:FBgn0032390; -.
DR eggNOG; ENOG502TCA4; Eukaryota.
DR HOGENOM; CLU_1200930_0_0_1; -.
DR InParanoid; Q9VKD6; -.
DR OMA; YTDRKAV; -.
DR OrthoDB; 1149430at2759; -.
DR PhylomeDB; Q9VKD6; -.
DR BioGRID-ORCS; 34610; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 34610; -.
DR PRO; PR:Q9VKD6; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032390; Expressed in secondary oocyte and 20 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0072687; C:meiotic spindle; IDA:FlyBase.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0032991; C:protein-containing complex; IDA:FlyBase.
DR GO; GO:0005819; C:spindle; IDA:FlyBase.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0008017; F:microtubule binding; IDA:FlyBase.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..231
FT /note="Augmin complex subunit dgt2"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438651"
FT COILED 128..199
FT /evidence="ECO:0000255"
SQ SEQUENCE 231 AA; 25845 MW; 09D0C4E25B10AE60 CRC64;
MDDPSATLLP EHSEDLRLAR DAELKKVLKL KLVLDELRRL DVGPNPSDEI KRALKLVSIG
SYARLGEMEG AGQEQVLGLP SGSSFPPLNY TDRKTVRTKL SAQLRTTLQP IAELCDRIRE
EFPDAFGQEA DLSCDQKEIL RLEEEHRSGL EKLVALLTRK CTLLKETAEL KLGPQLANEL
KLQQAQAQLV QTKAELLRGF FVHEAASRTE HSVKAHKEVE AHLDELLAAK K
