DGT4_DROME
ID DGT4_DROME Reviewed; 188 AA.
AC Q9W4M8; O97426; Q7K763;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Augmin complex subunit dgt4 {ECO:0000305};
DE AltName: Full=Dim gamma-tubulin 4 {ECO:0000303|PubMed:17412918};
GN Name=dgt4 {ECO:0000303|PubMed:17412918, ECO:0000312|FlyBase:FBgn0026085};
GN ORFNames=CG4865 {ECO:0000312|FlyBase:FBgn0026085};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:CAA21830.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R {ECO:0000312|EMBL:CAA21830.1};
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4] {ECO:0000312|EMBL:AAM51137.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM51137.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAM51137.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17412918; DOI=10.1126/science.1141314;
RA Goshima G., Wollman R., Goodwin S.S., Zhang N., Scholey J.M., Vale R.D.,
RA Stuurman N.;
RT "Genes required for mitotic spindle assembly in Drosophila S2 cells.";
RL Science 316:417-421(2007).
RN [6] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN THE AUGMIN COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=18443220; DOI=10.1083/jcb.200711053;
RA Goshima G., Mayer M., Zhang N., Stuurman N., Vale R.D.;
RT "Augmin: a protein complex required for centrosome-independent microtubule
RT generation within the spindle.";
RL J. Cell Biol. 181:421-429(2008).
RN [7] {ECO:0000305}
RP IDENTIFICATION IN THE AUGMIN COMPLEX, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=19369198; DOI=10.1073/pnas.0901587106;
RA Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C.,
RA Goshima G.;
RT "The augmin complex plays a critical role in spindle microtubule generation
RT for mitotic progression and cytokinesis in human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009).
CC -!- FUNCTION: As part of the augmin complex, plays a role in centrosome-
CC independent generation of spindle microtubules (PubMed:18443220). The
CC complex is required for mitotic spindle assembly through its
CC involvement in localizing gamma-tubulin to spindle microtubules
CC (PubMed:17412918). {ECO:0000269|PubMed:17412918,
CC ECO:0000269|PubMed:18443220}.
CC -!- SUBUNIT: Component of the augmin complex composed of dgt2, dgt3, dgt4,
CC dgt5, dgt6, msd1, msd5 and wac (PubMed:18443220, PubMed:19369198). The
CC complex interacts directly or indirectly with microtubules and is
CC required for centrosome-independent generation of spindle microtubules
CC (PubMed:18443220). {ECO:0000269|PubMed:18443220,
CC ECO:0000269|PubMed:19369198}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:17412918, ECO:0000269|PubMed:18443220}.
CC -!- MISCELLANEOUS: The name 'dim gamma-tubulin 4' derives from the
CC decreased gamma-tubulin staining of the spindle pole seen following
CC RNAi-mediated knockdown of dgt4 in S2 cells.
CC {ECO:0000303|PubMed:17412918}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA21830.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF45922.1; -; Genomic_DNA.
DR EMBL; AE014298; AGB95077.1; -; Genomic_DNA.
DR EMBL; AE014298; AGB95078.1; -; Genomic_DNA.
DR EMBL; AL033125; CAA21830.1; ALT_INIT; Genomic_DNA.
DR EMBL; AY119277; AAM51137.1; -; mRNA.
DR RefSeq; NP_001259231.1; NM_001272302.1.
DR RefSeq; NP_001259232.1; NM_001272303.1.
DR RefSeq; NP_572150.1; NM_131922.3.
DR AlphaFoldDB; Q9W4M8; -.
DR SMR; Q9W4M8; -.
DR IntAct; Q9W4M8; 10.
DR STRING; 7227.FBpp0305385; -.
DR PaxDb; Q9W4M8; -.
DR DNASU; 31361; -.
DR EnsemblMetazoa; FBtr0070642; FBpp0070610; FBgn0026085.
DR EnsemblMetazoa; FBtr0333183; FBpp0305385; FBgn0026085.
DR EnsemblMetazoa; FBtr0333184; FBpp0305386; FBgn0026085.
DR GeneID; 31361; -.
DR KEGG; dme:Dmel_CG4865; -.
DR UCSC; CG4865-RA; d. melanogaster.
DR CTD; 31361; -.
DR FlyBase; FBgn0026085; dgt4.
DR VEuPathDB; VectorBase:FBgn0026085; -.
DR eggNOG; ENOG502TBCP; Eukaryota.
DR HOGENOM; CLU_117821_0_0_1; -.
DR InParanoid; Q9W4M8; -.
DR OMA; DAKCEYQ; -.
DR OrthoDB; 1520210at2759; -.
DR PhylomeDB; Q9W4M8; -.
DR BioGRID-ORCS; 31361; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31361; -.
DR PRO; PR:Q9W4M8; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0026085; Expressed in secondary oocyte and 17 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005819; C:spindle; IDA:FlyBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007052; P:mitotic spindle organization; IMP:FlyBase.
DR GO; GO:0007088; P:regulation of mitotic nuclear division; IMP:FlyBase.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..188
FT /note="Augmin complex subunit dgt4"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438653"
FT COILED 141..163
FT /evidence="ECO:0000255"
SQ SEQUENCE 188 AA; 21425 MW; D65D8B0C0A250F7E CRC64;
METPPTPTTT TPPSLTTEGT MDDIQYLLHL EAMRRFQEDS RNVKRQVEEQ VRIWLDAKCE
YQRDFGRLAR LLKCGALQAA VDAHRVSDVN QVDQAAKDIA SLRSKLGSDL RPAILDSNDV
KQCLEHLNTT HKPRLNLCRQ QREFAQNQEA LRSLRTAVDG LENGMEMGMI QAMDRLVEDL
LPPRETNN
