DGT5_DROME
ID DGT5_DROME Reviewed; 685 AA.
AC Q7K4B4;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Augmin complex subunit dgt5 {ECO:0000305};
DE AltName: Full=Dim gamma-tubulin 5 {ECO:0000303|PubMed:17412918};
GN Name=dgt5 {ECO:0000303|PubMed:17412918, ECO:0000312|FlyBase:FBgn0033740};
GN ORFNames=CG8828 {ECO:0000312|FlyBase:FBgn0033740};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAK93442.1};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAK93442.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK93442.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAK93442.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17412918; DOI=10.1126/science.1141314;
RA Goshima G., Wollman R., Goodwin S.S., Zhang N., Scholey J.M., Vale R.D.,
RA Stuurman N.;
RT "Genes required for mitotic spindle assembly in Drosophila S2 cells.";
RL Science 316:417-421(2007).
RN [5] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN THE AUGMIN COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=18443220; DOI=10.1083/jcb.200711053;
RA Goshima G., Mayer M., Zhang N., Stuurman N., Vale R.D.;
RT "Augmin: a protein complex required for centrosome-independent microtubule
RT generation within the spindle.";
RL J. Cell Biol. 181:421-429(2008).
RN [6] {ECO:0000305}
RP IDENTIFICATION IN THE AUGMIN COMPLEX, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=19369198; DOI=10.1073/pnas.0901587106;
RA Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C.,
RA Goshima G.;
RT "The augmin complex plays a critical role in spindle microtubule generation
RT for mitotic progression and cytokinesis in human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009).
RN [7] {ECO:0000305}
RP SUBCELLULAR LOCATION.
RX PubMed=24829288; DOI=10.1098/rsob.140047;
RA Savoian M.S., Glover D.M.;
RT "Differing requirements for Augmin in male meiotic and mitotic spindle
RT formation in Drosophila.";
RL Open Biol. 4:140047-140047(2014).
CC -!- FUNCTION: As part of the augmin complex, plays a role in centrosome-
CC independent generation of spindle microtubules (PubMed:18443220). The
CC complex is required for mitotic spindle assembly through its
CC involvement in localizing gamma-tubulin to spindle microtubules
CC (PubMed:17412918). {ECO:0000269|PubMed:17412918,
CC ECO:0000269|PubMed:18443220}.
CC -!- SUBUNIT: Component of the augmin complex composed of dgt2, dgt3, dgt4,
CC dgt5, dgt6, msd1, msd5 and wac (PubMed:18443220, PubMed:19369198). The
CC complex interacts directly or indirectly with microtubules and is
CC required for centrosome-independent generation of spindle microtubules
CC (PubMed:18443220). {ECO:0000269|PubMed:18443220,
CC ECO:0000269|PubMed:19369198}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:17412918, ECO:0000269|PubMed:18443220}. Chromosome,
CC centromere, kinetochore {ECO:0000269|PubMed:18443220,
CC ECO:0000269|PubMed:24829288}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome {ECO:0000269|PubMed:24829288}.
CC Chromosome, centromere {ECO:0000269|PubMed:24829288}. Note=In mitotic
CC cells, detected on kinetochore microtubules but greatly reduced at the
CC spindle equator where only non-kinetochore microtubules are present
CC (PubMed:18443220). During male meiosis, localizes to kinetochores in a
CC microtubule-independent manner but does not concentrate on spindle
CC microtubules (PubMed:24829288). {ECO:0000269|PubMed:18443220,
CC ECO:0000269|PubMed:24829288}.
CC -!- MISCELLANEOUS: The name 'dim gamma-tubulin 5' derives from the
CC decreased gamma-tubulin staining of the spindle pole seen following
CC RNAi-mediated knockdown of dgt5 in S2 cells.
CC {ECO:0000303|PubMed:17412918}.
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DR EMBL; AE013599; AAF58505.1; -; Genomic_DNA.
DR EMBL; AE013599; AHN56147.1; -; Genomic_DNA.
DR EMBL; AY052018; AAK93442.1; -; mRNA.
DR RefSeq; NP_001286349.1; NM_001299420.1.
DR RefSeq; NP_610785.1; NM_136941.3.
DR AlphaFoldDB; Q7K4B4; -.
DR SMR; Q7K4B4; -.
DR DIP; DIP-48826N; -.
DR IntAct; Q7K4B4; 3.
DR STRING; 7227.FBpp0087059; -.
DR PaxDb; Q7K4B4; -.
DR EnsemblMetazoa; FBtr0087948; FBpp0087059; FBgn0033740.
DR EnsemblMetazoa; FBtr0339638; FBpp0308700; FBgn0033740.
DR GeneID; 36364; -.
DR KEGG; dme:Dmel_CG8828; -.
DR UCSC; CG8828-RA; d. melanogaster.
DR CTD; 36364; -.
DR FlyBase; FBgn0033740; dgt5.
DR VEuPathDB; VectorBase:FBgn0033740; -.
DR eggNOG; ENOG502TAUZ; Eukaryota.
DR HOGENOM; CLU_432251_0_0_1; -.
DR InParanoid; Q7K4B4; -.
DR OMA; CLANDSV; -.
DR OrthoDB; 579509at2759; -.
DR PhylomeDB; Q7K4B4; -.
DR SignaLink; Q7K4B4; -.
DR BioGRID-ORCS; 36364; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 36364; -.
DR PRO; PR:Q7K4B4; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033740; Expressed in egg cell and 13 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR GO; GO:0005819; C:spindle; IDA:FlyBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007052; P:mitotic spindle organization; IMP:FlyBase.
DR GO; GO:0007088; P:regulation of mitotic nuclear division; IMP:FlyBase.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW Cytoskeleton; Kinetochore; Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..685
FT /note="Augmin complex subunit dgt5"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438654"
FT COILED 87..165
FT /evidence="ECO:0000255"
FT COILED 342..379
FT /evidence="ECO:0000255"
SQ SEQUENCE 685 AA; 77978 MW; 4808C2FDEF7E3A91 CRC64;
MAYQEKITEF KNWATNLGCP PTALPTDDAL RRIFKSGSSL LLNQLQSRIQ PVDYVREVRE
NLLIAQVARY KDKMVPLASR SFQPPELQRY QKIQELKKHK EKANQQLTEA RKEYQKLSAG
IKTKNIQTIS AENRKQLLES KCNILDLKLE SLNKNYDQEL KNKIQILGTT PVKLSARNAS
EAQATRAVEQ ALKQLETFYG MCDDGNAVNN LAEAKQRLWD QMRSTFADIP NSLLLNVVMK
IKEEQLQHIM KLNESRGECT NDKPPLNNYE VKLLKTKADM LGLAAKYFAA QKELELKEER
FCQDYSVFVD KLQSKVYRFN GISLGDEENA DELISDYLVQ YNMRNFNRSQ NEFLREQIEQ
LRLELDAGAK QLENHDLKLG SVKQVYGDIN SSINRIQQDM VQLSQIKEKI LFSRNMMKNL
LDDMQAATQK QNAKSQLMST KLKVSNMSML GAESFCLAND SVFSSTKVEF DGNCSAINST
MRRSFDNKTL VPGGAASTTL MAASGATLPS HLLEFNTFLE IPLEKFSCTP RACSFLLSAN
PLIVEAQELA STVQLAPGYL LTPFGALQEV RKRILWASAI AAHTSELKLN LQPLIVDPHD
LRLKASRQHE EIDQLLDNLM AIGVKTQLQL EKAERIYQFL LENPLRRYVP PSKRYNNGSF
ADYESEFNLY YRMTTNGSSM RAPPN
