ID   ADA2_CHICK              Reviewed;          12 AA.
AC   P84520;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   11-DEC-2019, entry version 39.
DE   RecName: Full=Adenosine deaminase 2;
DE            EC=3.5.4.4;
DE   Flags: Fragment;
GN   Name=ADA2;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, ACTIVITY REGULATION,
RP   BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, AND GLYCOSYLATION.
RC   TISSUE=Liver {ECO:0000269|PubMed:14990221};
RX   PubMed=14990221; DOI=10.1016/j.cbpc.2003.11.010;
RA   Iwaki-Egawa S., Namiki C., Watanabe Y.;
RT   "Adenosine deaminase 2 from chicken liver: purification, characterization,
RT   and N-terminal amino acid sequence.";
RL   Comp. Biochem. Physiol. 137B:247-254(2004).
CC   -!- FUNCTION: Catalyzes the hydrolytic deamination of adenosine and 2-
CC       deoxyadenosine. Plays an important role in purine metabolism and in
CC       adenosine homeostasis. Modulates signaling by extracellular adenosine,
CC       and so contributes indirectly to cellular signaling events (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine + H(+) + H2O = inosine + NH4(+);
CC         Xref=Rhea:RHEA:24408, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16335, ChEBI:CHEBI:17596, ChEBI:CHEBI:28938; EC=3.5.4.4;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10104,
CC         ECO:0000269|PubMed:14990221};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- ACTIVITY REGULATION: Inhibited by adenosine deaminase inhibitor 2-DCF,
CC       but not by adenosine deaminase 1 inhibitor EHNA.
CC       {ECO:0000269|PubMed:14990221}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=144 uM for adenosine {ECO:0000269|PubMed:14990221};
CC         KM=333 uM for 2-deoxyadenosine {ECO:0000269|PubMed:14990221};
CC       pH dependence:
CC         Optimum pH is 6.5. {ECO:0000269|PubMed:14990221};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:14990221}.
CC   -!- PTM: N-glycosylated. {ECO:0000269|PubMed:14990221}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       Adenosine and AMP deaminases family. {ECO:0000303|PubMed:14990221}.
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DR   SABIO-RK; P84520; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0004000; F:adenosine deaminase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009117; P:nucleotide metabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Hydrolase; Nucleotide metabolism;
KW   Reference proteome.
FT   CHAIN           1..>12
FT                   /note="Adenosine deaminase 2"
FT                   /id="PRO_0000194359"
FT   NON_TER         12
FT                   /evidence="ECO:0000303|PubMed:14990221"
SQ   SEQUENCE   12 AA;  1466 MW;  8A03AE7AB2D69735 CRC64;
     TPLWSLMQDL MM