DHAA_AGRFC
ID DHAA_AGRFC Reviewed; 304 AA.
AC Q8U671;
DT 22-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Haloalkane dehalogenase {ECO:0000255|HAMAP-Rule:MF_01231};
DE EC=3.8.1.5 {ECO:0000255|HAMAP-Rule:MF_01231};
GN Name=dhaA {ECO:0000255|HAMAP-Rule:MF_01231}; Synonyms=dha;
GN OrderedLocusNames=Atu6064; ORFNames=AGR_pTi_130;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC halogenated aliphatic compounds, leading to the formation of the
CC corresponding primary alcohols, halide ions and protons.
CC {ECO:0000255|HAMAP-Rule:MF_01231}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01231};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01231}.
CC -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01231}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK91024.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE007871; AAK91024.2; ALT_INIT; Genomic_DNA.
DR PIR; AF3235; AF3235.
DR RefSeq; NP_396583.2; NC_003065.3.
DR PDB; 3WI7; X-ray; 1.70 A; A/B=1-304.
DR PDB; 3WIB; X-ray; 1.95 A; A/B=1-304.
DR PDBsum; 3WI7; -.
DR PDBsum; 3WIB; -.
DR AlphaFoldDB; Q8U671; -.
DR SMR; Q8U671; -.
DR ESTHER; agrtu-DHAA; Haloalkane_dehalogenase-HLD2.
DR EnsemblBacteria; AAK91024; AAK91024; Atu6064.
DR KEGG; atu:Atu6064; -.
DR PATRIC; fig|176299.10.peg.5271; -.
DR HOGENOM; CLU_020336_13_3_5; -.
DR OMA; TLFCQDW; -.
DR BRENDA; 3.8.1.5; 200.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.1820; -; 1.
DR HAMAP; MF_01231; Haloalk_dehal_type2; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR023594; Haloalkane_dehalogenase_2.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Plasmid; Reference proteome.
FT CHAIN 1..304
FT /note="Haloalkane dehalogenase"
FT /id="PRO_0000216779"
FT DOMAIN 42..154
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT ACT_SITE 114
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
FT ACT_SITE 138
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
FT ACT_SITE 280
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
FT HELIX 14..16
FT /evidence="ECO:0007829|PDB:3WI7"
FT STRAND 21..27
FT /evidence="ECO:0007829|PDB:3WI7"
FT STRAND 30..37
FT /evidence="ECO:0007829|PDB:3WI7"
FT STRAND 40..46
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 53..56
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 57..62
FT /evidence="ECO:0007829|PDB:3WI7"
FT TURN 63..65
FT /evidence="ECO:0007829|PDB:3WI7"
FT STRAND 66..72
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 89..103
FT /evidence="ECO:0007829|PDB:3WI7"
FT STRAND 107..114
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 116..126
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 128..130
FT /evidence="ECO:0007829|PDB:3WI7"
FT STRAND 131..139
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 146..148
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 151..160
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 165..170
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 175..178
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 180..183
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 191..198
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 205..207
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 208..216
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 224..231
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 234..239
FT /evidence="ECO:0007829|PDB:3WI7"
FT STRAND 244..251
FT /evidence="ECO:0007829|PDB:3WI7"
FT STRAND 253..255
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 257..266
FT /evidence="ECO:0007829|PDB:3WI7"
FT STRAND 270..280
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 282..285
FT /evidence="ECO:0007829|PDB:3WI7"
FT HELIX 287..301
FT /evidence="ECO:0007829|PDB:3WI7"
SQ SEQUENCE 304 AA; 34390 MW; 4783D160D4C86DFA CRC64;
MKEHRHMTEK SPHSAFGDGA KAYDVPAFGL QIHTVEHGSG APIVFLHGNP TSSYLWRHIF
RRLHGHGRLL AVDLIGYGQS SKPDIEYTLE NQQRYVDAWF DALDLRNVTL VLQDYGAAFG
LNWASRNPDR VRAVAFFEPV LRNIDSVDLS PEFVTRRAKL RQPGEGEIFV QQENRFLTEL
FPWFFLTPLA PEDLRQYQTP FPTPHSRKAI LAGPRNLPVD GEPASTVAFL EQAVNWLNTS
DTPKLLLTFK PGFLLTDAIL KWSQVTIRNL EIEAAGAGIH FVQEEQPETI ARLLDAWLTR
IAGN
