DHAA_MYCTA
ID DHAA_MYCTA Reviewed; 300 AA.
AC A5U5S9;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Haloalkane dehalogenase {ECO:0000255|HAMAP-Rule:MF_01231};
DE EC=3.8.1.5 {ECO:0000255|HAMAP-Rule:MF_01231};
GN Name=dhaA {ECO:0000255|HAMAP-Rule:MF_01231}; OrderedLocusNames=MRA_2608;
OS Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=419947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25177 / H37Ra;
RX PubMed=18584054; DOI=10.1371/journal.pone.0002375;
RA Zheng H., Lu L., Wang B., Pu S., Zhang X., Zhu G., Shi W., Zhang L.,
RA Wang H., Wang S., Zhao G., Zhang Y.;
RT "Genetic basis of virulence attenuation revealed by comparative genomic
RT analysis of Mycobacterium tuberculosis strain H37Ra versus H37Rv.";
RL PLoS ONE 3:E2375-E2375(2008).
CC -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC halogenated aliphatic compounds, leading to the formation of the
CC corresponding primary alcohols, halide ions and protons.
CC {ECO:0000255|HAMAP-Rule:MF_01231}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01231};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01231}.
CC -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01231}.
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DR EMBL; CP000611; ABQ74379.1; -; Genomic_DNA.
DR RefSeq; WP_003413363.1; NZ_CP016972.1.
DR AlphaFoldDB; A5U5S9; -.
DR SMR; A5U5S9; -.
DR STRING; 419947.MRA_2608; -.
DR ESTHER; myctu-linb; Haloalkane_dehalogenase-HLD2.
DR EnsemblBacteria; ABQ74379; ABQ74379; MRA_2608.
DR GeneID; 45426581; -.
DR KEGG; mra:MRA_2608; -.
DR eggNOG; COG0596; Bacteria.
DR HOGENOM; CLU_020336_13_3_11; -.
DR OMA; TLEWPRQ; -.
DR OrthoDB; 1890883at2; -.
DR Proteomes; UP000001988; Chromosome.
DR GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.1820; -; 1.
DR HAMAP; MF_01231; Haloalk_dehal_type2; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR000639; Epox_hydrolase-like.
DR InterPro; IPR023594; Haloalkane_dehalogenase_2.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR PRINTS; PR00412; EPOXHYDRLASE.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 3: Inferred from homology;
KW Hydrolase.
FT CHAIN 1..300
FT /note="Haloalkane dehalogenase"
FT /id="PRO_1000066844"
FT DOMAIN 32..155
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT ACT_SITE 109
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
FT ACT_SITE 133
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
FT ACT_SITE 273
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
SQ SEQUENCE 300 AA; 33728 MW; E8F4854749F22562 CRC64;
MTAFGVEPYG QPKYLEIAGK RMAYIDEGKG DAIVFQHGNP TSSYLWRNIM PHLEGLGRLV
ACDLIGMGAS DKLSPSGPDR YSYGEQRDFL FALWDALDLG DHVVLVLHDW GSALGFDWAN
QHRDRVQGIA FMEAIVTPMT WADWPPAVRG VFQGFRSPQG EPMALEHNIF VERVLPGAIL
RQLSDEEMNH YRRPFVNGGE DRRPTLSWPR NLPIDGEPAE VVALVNEYRS WLEETDMPKL
FINAEPGAII TGRIRDYVRS WPNQTEITVP GVHFVQEDSP EEIGAAIAQF VRRLRSAAGV