DHAA_RHILO
ID DHAA_RHILO Reviewed; 309 AA.
AC Q98C03;
DT 22-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Haloalkane dehalogenase {ECO:0000255|HAMAP-Rule:MF_01231};
DE EC=3.8.1.5 {ECO:0000255|HAMAP-Rule:MF_01231};
GN Name=dhaA {ECO:0000255|HAMAP-Rule:MF_01231}; OrderedLocusNames=mlr5354;
OS Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)
OS (Mesorhizobium loti (strain MAFF 303099)).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Phyllobacteriaceae; Mesorhizobium.
OX NCBI_TaxID=266835;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 29417 / CECT 9101 / MAFF 303099;
RX PubMed=11214968; DOI=10.1093/dnares/7.6.331;
RA Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S.,
RA Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y.,
RA Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y.,
RA Nakayama S., Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M.,
RA Tabata S.;
RT "Complete genome structure of the nitrogen-fixing symbiotic bacterium
RT Mesorhizobium loti.";
RL DNA Res. 7:331-338(2000).
CC -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC halogenated aliphatic compounds, leading to the formation of the
CC corresponding primary alcohols, halide ions and protons.
CC {ECO:0000255|HAMAP-Rule:MF_01231}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01231};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01231}.
CC -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01231}.
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DR EMBL; BA000012; BAB51818.1; -; Genomic_DNA.
DR AlphaFoldDB; Q98C03; -.
DR SMR; Q98C03; -.
DR STRING; 266835.14025217; -.
DR ESTHER; rhilo-dhaa; Haloalkane_dehalogenase-HLD2.
DR EnsemblBacteria; BAB51818; BAB51818; BAB51818.
DR KEGG; mlo:mlr5354; -.
DR eggNOG; COG0596; Bacteria.
DR HOGENOM; CLU_020336_13_3_5; -.
DR OMA; TLFCQDW; -.
DR BRENDA; 3.8.1.5; 3243.
DR Proteomes; UP000000552; Chromosome.
DR GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.1820; -; 1.
DR HAMAP; MF_01231; Haloalk_dehal_type2; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR000639; Epox_hydrolase-like.
DR InterPro; IPR023594; Haloalkane_dehalogenase_2.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR PRINTS; PR00412; EPOXHYDRLASE.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 3: Inferred from homology;
KW Hydrolase.
FT CHAIN 1..309
FT /note="Haloalkane dehalogenase"
FT /id="PRO_0000216784"
FT DOMAIN 37..148
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT ACT_SITE 110
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
FT ACT_SITE 134
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
FT ACT_SITE 278
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
SQ SEQUENCE 309 AA; 34201 MW; A59C6434FD37053F CRC64;
MSSKANPPQP VATAPKRSQI PILDSTMSYV EAGASGPTVL FLHGNPTSSH IWRNIIPHVA
PFGRCIAPDL IGYGQSGKPD IDYRFFDHVR YLDAFLDALD IRDVLLVAQD WGTALAFHLA
ARRPQRVLGL AFMEFIRPFE RWEDFHQRPQ AREMFKALRT PGVGEKLVLE DNVFVEKVLP
ASVLRAMSDD EMDVYRAPFP TPQSRKPVLR LPREMPIEGQ PADVAAISAH DHRALRLSTY
PKLLFAGDPG ALIGPQAARE FAAGLKNCSF INLGPGAHYL QEDHADAIGR AIASWLPEVV
LANQTDELA