位置:首页 > 蛋白库 > DHAA_RHILO
DHAA_RHILO
ID   DHAA_RHILO              Reviewed;         309 AA.
AC   Q98C03;
DT   22-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Haloalkane dehalogenase {ECO:0000255|HAMAP-Rule:MF_01231};
DE            EC=3.8.1.5 {ECO:0000255|HAMAP-Rule:MF_01231};
GN   Name=dhaA {ECO:0000255|HAMAP-Rule:MF_01231}; OrderedLocusNames=mlr5354;
OS   Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)
OS   (Mesorhizobium loti (strain MAFF 303099)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Phyllobacteriaceae; Mesorhizobium.
OX   NCBI_TaxID=266835;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 29417 / CECT 9101 / MAFF 303099;
RX   PubMed=11214968; DOI=10.1093/dnares/7.6.331;
RA   Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y.,
RA   Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y.,
RA   Nakayama S., Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M.,
RA   Tabata S.;
RT   "Complete genome structure of the nitrogen-fixing symbiotic bacterium
RT   Mesorhizobium loti.";
RL   DNA Res. 7:331-338(2000).
CC   -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC       halogenated aliphatic compounds, leading to the formation of the
CC       corresponding primary alcohols, halide ions and protons.
CC       {ECO:0000255|HAMAP-Rule:MF_01231}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC         H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01231};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01231}.
CC   -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01231}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000012; BAB51818.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q98C03; -.
DR   SMR; Q98C03; -.
DR   STRING; 266835.14025217; -.
DR   ESTHER; rhilo-dhaa; Haloalkane_dehalogenase-HLD2.
DR   EnsemblBacteria; BAB51818; BAB51818; BAB51818.
DR   KEGG; mlo:mlr5354; -.
DR   eggNOG; COG0596; Bacteria.
DR   HOGENOM; CLU_020336_13_3_5; -.
DR   OMA; TLFCQDW; -.
DR   BRENDA; 3.8.1.5; 3243.
DR   Proteomes; UP000000552; Chromosome.
DR   GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   HAMAP; MF_01231; Haloalk_dehal_type2; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   InterPro; IPR023594; Haloalkane_dehalogenase_2.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..309
FT                   /note="Haloalkane dehalogenase"
FT                   /id="PRO_0000216784"
FT   DOMAIN          37..148
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        110
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
FT   ACT_SITE        134
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
FT   ACT_SITE        278
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01231"
SQ   SEQUENCE   309 AA;  34201 MW;  A59C6434FD37053F CRC64;
     MSSKANPPQP VATAPKRSQI PILDSTMSYV EAGASGPTVL FLHGNPTSSH IWRNIIPHVA
     PFGRCIAPDL IGYGQSGKPD IDYRFFDHVR YLDAFLDALD IRDVLLVAQD WGTALAFHLA
     ARRPQRVLGL AFMEFIRPFE RWEDFHQRPQ AREMFKALRT PGVGEKLVLE DNVFVEKVLP
     ASVLRAMSDD EMDVYRAPFP TPQSRKPVLR LPREMPIEGQ PADVAAISAH DHRALRLSTY
     PKLLFAGDPG ALIGPQAARE FAAGLKNCSF INLGPGAHYL QEDHADAIGR AIASWLPEVV
     LANQTDELA
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024