ID   DHAB_CITFR              Reviewed;         555 AA.
AC   P45514;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Glycerol dehydratase large subunit;
DE            EC=4.2.1.30;
GN   Name=dhaB;
OS   Citrobacter freundii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 6750 / DSM 30040 / NCIB 8173 / M8BK;
RX   PubMed=8824629; DOI=10.1128/jb.178.19.5793-5796.1996;
RA   Seyfried M., Daniel R., Gottschalk G.;
RT   "Cloning, sequencing, and overexpression of the genes encoding coenzyme
RT   B12-dependent glycerol dehydratase of Citrobacter freundii.";
RL   J. Bacteriol. 178:5793-5796(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glycerol = 3-hydroxypropanal + H2O; Xref=Rhea:RHEA:19765,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:17754, ChEBI:CHEBI:17871; EC=4.2.1.30;
CC   -!- COFACTOR:
CC       Name=adenosylcob(III)alamin; Xref=ChEBI:CHEBI:18408;
CC   -!- SUBUNIT: Probably consists of three subunits: large, medium, and small.
CC   -!- SIMILARITY: Belongs to the diol/glycerol dehydratase large subunit
CC       family. {ECO:0000305}.
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DR   EMBL; U09771; AAB48850.1; -; Genomic_DNA.
DR   AlphaFoldDB; P45514; -.
DR   SMR; P45514; -.
DR   STRING; 1333848.CFNIH1_02610; -.
DR   PRIDE; P45514; -.
DR   BioCyc; MetaCyc:MON-4485; -.
DR   GO; GO:0031419; F:cobalamin binding; IEA:UniProtKB-KW.
DR   GO; GO:0046405; F:glycerol dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd03687; Dehydratase_LU; 1.
DR   Gene3D; 3.20.20.350; -; 1.
DR   InterPro; IPR016176; Cbl-dep_enz_cat.
DR   InterPro; IPR036999; Diol/glycerol_deHase_lsu_sf.
DR   InterPro; IPR003206; Diol/glycerol_deHydtase_lsu.
DR   Pfam; PF02286; Dehydratase_LU; 1.
DR   PIRSF; PIRSF018507; Prpndl_dhdrts_lg; 1.
DR   SUPFAM; SSF51703; SSF51703; 1.
PE   1: Evidence at protein level;
KW   Cobalamin; Cobalt; Direct protein sequencing; Glycerol metabolism; Lyase.
FT   CHAIN           1..555
FT                   /note="Glycerol dehydratase large subunit"
FT                   /id="PRO_0000079884"
SQ   SEQUENCE   555 AA;  60472 MW;  C1D5E8CC3806B989 CRC64;
     MRRSKRFEVL AQRPVNQDGL IGEWPEEGLI AMESPYDPAS SVKVENGRIV ELDGKSRAEF
     DMIDRFIADY AINVPEAERA MQLDALEIAR MLVDIHVSRE EIIAITTAIT PAKRLEVMAQ
     MNVVEMMMAL QKMRARRTPS NQCHVTNLKD NPVQIAADAA EAGIRGFSEQ ETTVGIARYA
     PFNALALLVG SQCGAPGVLT QCSVEEATEL ELGMRGLTSY AETVSVYGTE SVFTDGDDTP
     WSKAFLASAY ASRGLKMRYT SGTGSEALMG YSESKSMLYL ESRCIFITKG AGVQGLQNGA
     VSCIGMTGAV PSGIRAVLAE NLIASMLDLE VASANDQTFS HSDIRRTART LMQMLPGTDF
     IFSGYSAVPN YDNMFAGSNF DAEDFDDYNI LQRDLMVDGG LRPVTEEETI AIRNKAARAI
     QAVFRELGLP LISDEEVDAA TYAHGSKDMP ARNVVEDLAA VEEMMKRNIT GLDIVGALSS
     SGFEDIASNI LNMLRQRVTG DYLQTSAILD RQFDVVSAVN DINDYQGPGT GYRISAERWA
     EIKNIAGVVQ PGSIE