ID   DHAR_CITFR              Reviewed;         641 AA.
AC   P45512;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Glycerol metabolism operon regulatory protein {ECO:0000303|PubMed:7635824};
GN   Name=dhaR {ECO:0000303|PubMed:7635824};
OS   Citrobacter freundii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 6750 / DSM 30040 / NCIB 8173 / M8BK;
RX   PubMed=7635824; DOI=10.1128/jb.177.15.4392-4401.1995;
RA   Daniel R., Stuertz K., Gottschalk G.;
RT   "Biochemical and molecular characterization of the oxidative branch of
RT   glycerol utilization by Citrobacter freundii.";
RL   J. Bacteriol. 177:4392-4401(1995).
CC   -!- FUNCTION: Transcriptional activator of the glycerol utilization dha
CC       operon. {ECO:0000269|PubMed:7635824}.
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DR   EMBL; U09771; AAB48845.1; -; Genomic_DNA.
DR   RefSeq; WP_016154585.1; NZ_CABHHG010000021.1.
DR   AlphaFoldDB; P45512; -.
DR   SMR; P45512; -.
DR   STRING; 1333848.CFNIH1_02635; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:UniProt.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00130; PAS; 1.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000014; PAS.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   InterPro; IPR013767; PAS_fold.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00989; PAS; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00091; PAS; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF55785; SSF55785; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; ATP-binding; DNA-binding; Glycerol metabolism;
KW   Nucleotide-binding; Transcription; Transcription regulation.
FT   CHAIN           1..641
FT                   /note="Glycerol metabolism operon regulatory protein"
FT                   /id="PRO_0000081319"
FT   DOMAIN          52..189
FT                   /note="GAF"
FT                   /evidence="ECO:0000250|UniProtKB:P76016"
FT   DOMAIN          203..265
FT                   /note="PAS"
FT                   /evidence="ECO:0000250|UniProtKB:P76016"
FT   DOMAIN          327..552
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   REGION          1..318
FT                   /note="Sensor domain"
FT                   /evidence="ECO:0000250|UniProtKB:P76016"
FT   BINDING         355..362
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         415..424
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   641 AA;  71436 MW;  7E5089141277256E CRC64;
     MTTHTQDIGK EVSSVIAQSW HRCSKFMQRE TWQAPHQAQG LTFESICRRK TALLTIAQAA
     LEDAWEFMDG RPCALLILDE SACILSRCGD PQTVEQLAEL GFRDGSYCAE SIIGSCALSL
     ATMPGQPTKT SGDQHFKQAL HPWSFCSTPV FDNHGRLFGS ISLCCLVEHE CVSDLSLTLA
     IAREVGNSLL TDSLLAESNR HLNQMYGLLE SMDDGVMAWN EQGVLQFLNA RAALLLHLDA
     QASQGKNIND LLNLPMLLRR AIKHARGLNH VEVTFESQHQ FVDAVITLKP IVEEQGNSFI
     LLLHPVEQMR QLMTSQLGKV SHTFEQMSTD DPETRRLIHF GRQAARGSFP ILLCGEEGVG
     KELLSQAIHN ESERASGPYI AVNCQLYANS VLGQDFMGSA PTDDENGRLS RLELANGGTL
     FLEKIEYLAP ELQSALLQVI KQGVLTRLDA RRLIPVDVKV IATTTVDLAN LVEQNRFSRQ
     LYYALHSFEI VIPPLRARRN SIPSLIYTRL NSLKKRFSSS LKIDDDALAQ LVAYSWPGND
     FELNSIIENI AISSDNGHIR LSNLPDYLFA ERPGLETASS LLPASLTFTA IEKEAIIHAA
     RVTSGRVQEM SQLLNIGRTT LWRKMKQYDI DASQFKRKHL E