ID   DHAS_LACLA              Reviewed;         187 AA.
AC   Q9CIV9;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=HTH-type dhaKLM operon transcriptional activator DhaS;
GN   Name=dhaS; Synonyms=dhaR; OrderedLocusNames=LL0247; ORFNames=L45062;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), FUNCTION, AND SUBUNIT.
RX   PubMed=16760471; DOI=10.1074/jbc.m603486200;
RA   Christen S., Srinivas A., Baehler P., Zeller A., Pridmore D.,
RA   Bieniossek C., Baumann U., Erni B.;
RT   "Regulation of the Dha operon of Lactococcus lactis: a deviation from the
RT   rule followed by the Tetr family of transcription regulators.";
RL   J. Biol. Chem. 281:23129-23137(2006).
CC   -!- FUNCTION: In complex with DhaQ, upon activation by dihydroxyacetone,
CC       activates transcription of the dhaKLM operon. Binds the inverted repeat
CC       sequence 5'-GGACACATN(6)ATTTGTCC-3' located upstream of and partially
CC       overlapping with the -35 promoter sequence of the dhaKLM operon
CC       promoter. {ECO:0000269|PubMed:16760471}.
CC   -!- SUBUNIT: Homodimer. Interacts with a homodimer of DhaQ.
CC       {ECO:0000269|PubMed:16760471}.
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DR   EMBL; AE005176; AAK04345.1; -; Genomic_DNA.
DR   PIR; G86655; G86655.
DR   PDB; 2IU5; X-ray; 1.60 A; A/B=1-187.
DR   PDBsum; 2IU5; -.
DR   AlphaFoldDB; Q9CIV9; -.
DR   SMR; Q9CIV9; -.
DR   STRING; 272623.L45062; -.
DR   PaxDb; Q9CIV9; -.
DR   EnsemblBacteria; AAK04345; AAK04345; L45062.
DR   KEGG; lla:L45062; -.
DR   eggNOG; COG1309; Bacteria.
DR   HOGENOM; CLU_087539_2_2_9; -.
DR   OMA; SWEESFV; -.
DR   EvolutionaryTrace; Q9CIV9; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   InterPro; IPR039532; TetR_C_Firmicutes.
DR   InterPro; IPR012738; Tscrpt_reg_DhaS.
DR   Pfam; PF14278; TetR_C_8; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   TIGRFAMs; TIGR02366; DHAK_reg; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..187
FT                   /note="HTH-type dhaKLM operon transcriptional activator
FT                   DhaS"
FT                   /id="PRO_0000270533"
FT   DOMAIN          12..72
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        35..54
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   HELIX           13..28
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           31..33
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           36..43
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           47..52
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           57..73
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           82..95
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           97..106
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           110..132
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           139..163
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           168..170
FT                   /evidence="ECO:0007829|PDB:2IU5"
FT   HELIX           171..183
FT                   /evidence="ECO:0007829|PDB:2IU5"
SQ   SEQUENCE   187 AA;  22481 MW;  FFECB2C823630D15 CRC64;
     MSAFFLNMKK SIITQKIIAK AFKDLMQSNA YHQISVSDIM QTAKIRRQTF YNYFQNQEEL
     LSWIFENDFA ELINDNSDYY GWQNELLLLL RYLDENQIFY QKIFVIDKNF EHFFLIQWEN
     LLDKVIFDQE KKSDYHWSDL EKSFICRYNA AAICAITRES IIRGNSLEKL YSQIVNLLLA
     QIKIFES