ID   DHAT_KLEPN              Reviewed;         387 AA.
AC   Q59477;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=1,3-propanediol dehydrogenase {ECO:0000250|UniProtKB:P45513};
DE            EC=1.1.1.202 {ECO:0000250|UniProtKB:P45513};
DE   AltName: Full=1,3-propanediol oxidoreductase {ECO:0000250|UniProtKB:P45513};
DE   AltName: Full=3-hydroxypropionaldehyde reductase {ECO:0000250|UniProtKB:P45513};
GN   Name=dhaT;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 25955;
RA   Skraly F.A., Willard B.L., Cameron D.C.;
RL   Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + propane-1,3-diol = 3-hydroxypropanal + H(+) + NADH;
CC         Xref=Rhea:RHEA:23188, ChEBI:CHEBI:15378, ChEBI:CHEBI:16109,
CC         ChEBI:CHEBI:17871, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=1.1.1.202; Evidence={ECO:0000250|UniProtKB:P45513};
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC         Evidence={ECO:0000250|UniProtKB:P45513};
CC   -!- SUBUNIT: Homooctamer. {ECO:0000250|UniProtKB:P45513}.
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U30903; AAA74260.1; -; Genomic_DNA.
DR   RefSeq; WP_004150927.1; NZ_WYAM01000014.1.
DR   PDB; 3BFJ; X-ray; 2.70 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T=1-387.
DR   PDBsum; 3BFJ; -.
DR   AlphaFoldDB; Q59477; -.
DR   SMR; Q59477; -.
DR   PRIDE; Q59477; -.
DR   OMA; KMTIISK; -.
DR   OrthoDB; 1456634at2; -.
DR   BRENDA; 1.1.1.202; 2814.
DR   EvolutionaryTrace; Q59477; -.
DR   GO; GO:0047516; F:1,3-propanediol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   InterPro; IPR001670; ADH_Fe/GldA.
DR   InterPro; IPR018211; ADH_Fe_CS.
DR   InterPro; IPR039697; Alcohol_dehydrogenase_Fe.
DR   PANTHER; PTHR11496; PTHR11496; 1.
DR   Pfam; PF00465; Fe-ADH; 1.
DR   PROSITE; PS00913; ADH_IRON_1; 1.
DR   PROSITE; PS00060; ADH_IRON_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Iron; NAD; Oxidoreductase.
FT   CHAIN           1..387
FT                   /note="1,3-propanediol dehydrogenase"
FT                   /id="PRO_0000087842"
SQ   SEQUENCE   387 AA;  41466 MW;  37E7BD4CDF2EC409 CRC64;
     MSYRMFDYLV PNVNFFGPNA ISVVGERCQL LGGKKALLVT DKGLRAIKDG AVDKTLHYLR
     EAGIEVAIFD GVEPNPKDTN VRDGLAVFRR EQCDIIVTVG GGSPHDCGKG IGIAATHEGD
     LYQYAGIETL TNPLPPIVAV NTTAGTASEV TRHCVLTNTE TKVKFVIVSW RNLPSVSIND
     PLLMIGKPAA LTAATGMDAL THAVEAYISK DANPVTDAAA MQAIRLIARN LRQAVALGSN
     LQARENMAYA SLLAGMAFNN ANLGYVHAMA HQLGGLYDMP HGVANAVLLP HVARYNLIAN
     PEKFADIAEL MGENITGLST LDAAEKAIAA ITRLSMDIGI PQHLRDLGVK EADFPYMAEM
     ALKDGNAFSN PRKGNEQEIA AIFRQAF