ID   DHB14_BOVIN             Reviewed;         270 AA.
AC   Q9MYP6;
DT   11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=17-beta-hydroxysteroid dehydrogenase 14;
DE            Short=17-beta-HSD 14;
DE            EC=1.1.1.62 {ECO:0000250|UniProtKB:Q9BPX1};
DE   AltName: Full=17-beta-hydroxysteroid dehydrogenase DHRS10;
DE   AltName: Full=Dehydrogenase/reductase SDR family member 10;
DE   AltName: Full=Retinal short-chain dehydrogenase/reductase retSDR3;
GN   Name=HSD17B14; Synonyms=DHRS10, SDR3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Retina;
RA   Haeseleer F., Palczewski K.;
RL   Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Has NAD-dependent 17-beta-hydroxysteroid dehydrogenase
CC       activity. Converts oestradiol to oestrone. The physiological substrate
CC       is not known. Acts on oestradiol and 5-androstene-3-beta,17-beta-diol
CC       (in vitro). {ECO:0000250|UniProtKB:Q9BPX1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=17beta-estradiol + NAD(+) = estrone + H(+) + NADH;
CC         Xref=Rhea:RHEA:24612, ChEBI:CHEBI:15378, ChEBI:CHEBI:16469,
CC         ChEBI:CHEBI:17263, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.62;
CC         Evidence={ECO:0000250|UniProtKB:Q9BPX1};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:24613;
CC         Evidence={ECO:0000250|UniProtKB:Q9BPX1};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=androst-5-en-3beta,17beta-diol + NAD(+) = 3beta-
CC         hydroxyandrost-5-en-17-one + H(+) + NADH; Xref=Rhea:RHEA:46220,
CC         ChEBI:CHEBI:2710, ChEBI:CHEBI:15378, ChEBI:CHEBI:28689,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         Evidence={ECO:0000250|UniProtKB:Q9BPX1};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:46221;
CC         Evidence={ECO:0000250|UniProtKB:Q9BPX1};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q9BPX1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BPX1}.
CC   -!- TISSUE SPECIFICITY: Detected in retina.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF202997; AAF62401.1; -; mRNA.
DR   EMBL; AF238859; AAF44666.1; -; mRNA.
DR   RefSeq; NP_777126.1; NM_174701.2.
DR   AlphaFoldDB; Q9MYP6; -.
DR   SMR; Q9MYP6; -.
DR   IntAct; Q9MYP6; 1.
DR   STRING; 9913.ENSBTAP00000012090; -.
DR   PaxDb; Q9MYP6; -.
DR   PeptideAtlas; Q9MYP6; -.
DR   Ensembl; ENSBTAT00000012090; ENSBTAP00000012090; ENSBTAG00000009174.
DR   GeneID; 282652; -.
DR   KEGG; bta:282652; -.
DR   CTD; 51171; -.
DR   VEuPathDB; HostDB:ENSBTAG00000009174; -.
DR   VGNC; VGNC:29972; HSD17B14.
DR   eggNOG; KOG0725; Eukaryota.
DR   GeneTree; ENSGT00940000161346; -.
DR   HOGENOM; CLU_010194_1_0_1; -.
DR   InParanoid; Q9MYP6; -.
DR   OMA; QHGKIDN; -.
DR   OrthoDB; 1053465at2759; -.
DR   TreeFam; TF354307; -.
DR   Proteomes; UP000009136; Chromosome 18.
DR   Bgee; ENSBTAG00000009174; Expressed in caput epididymis and 89 other tissues.
DR   ExpressionAtlas; Q9MYP6; baseline and differential.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0044594; F:17-beta-hydroxysteroid dehydrogenase (NAD+) activity; IEA:RHEA.
DR   GO; GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR   GO; GO:0047045; F:testosterone 17-beta-dehydrogenase (NADP+) activity; IEA:Ensembl.
DR   GO; GO:0006706; P:steroid catabolic process; IBA:GO_Central.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Lipid metabolism; NAD; Oxidoreductase; Reference proteome;
KW   Steroid metabolism.
FT   CHAIN           1..270
FT                   /note="17-beta-hydroxysteroid dehydrogenase 14"
FT                   /id="PRO_0000054653"
FT   ACT_SITE        154
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         13..40
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         141
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         158
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   270 AA;  28421 MW;  0A7E6F84C289FF1C CRC64;
     MAMGTRYAGK VVIVTGGGRG IGAGIVRAFV ESGAQVVICD KDEARGRAVE RELPGTVFLL
     CDVTREEDVR TLVSETIRRF GRLDCIVNNA GYHPPPQWPE ETSAQGFRQL LELNLLGTYT
     LTKLALPHLR KSRGNVINIS SLVGAIGQSQ AVPYVATKGA VTAMTKALAL DESQYGVRVN
     CISPGNIWTP LWEELAASTP DPTATIREGT LAQPLGRMGQ PAEVAAAAVF LASEATFCTG
     TELLVTGGAE LGYGRKAGQA APAEAPTTPS