ID   ADAD2_MOUSE             Reviewed;         561 AA.
AC   Q9D5P4; B2RQL4;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-FEB-2022, sequence version 2.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Adenosine deaminase domain-containing protein 2 {ECO:0000305};
GN   Name=Adad2 {ECO:0000312|MGI:MGI:1923023};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=32665638; DOI=10.1038/s41598-020-67834-5;
RA   Snyder E., Chukrallah L., Seltzer K., Goodwin L., Braun R.E.;
RT   "ADAD1 and ADAD2, testis-specific adenosine deaminase domain-containing
RT   proteins, are required for male fertility.";
RL   Sci. Rep. 10:11536-11536(2020).
CC   -!- FUNCTION: Required for male fertility and normal male germ cell
CC       differentiation. {ECO:0000269|PubMed:32665638}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:32665638}. Cytoplasm
CC       {ECO:0000269|PubMed:32665638}. Note=Diffusely cytoplasmic early in
CC       pachytene spermatocytes and coalesces into several perinuclear granules
CC       by late pachynema. {ECO:0000269|PubMed:32665638}.
CC   -!- TISSUE SPECIFICITY: Testis-specific (at protein level).
CC       {ECO:0000269|PubMed:32665638}.
CC   -!- DISRUPTION PHENOTYPE: Male mice are sterile and show defects in germ-
CC       cell development. {ECO:0000269|PubMed:32665638}.
CC   -!- SIMILARITY: Belongs to the ADAD family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB29697.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAB29697.1; Type=Frameshift; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AK015063; BAB29697.1; ALT_SEQ; mRNA.
DR   EMBL; AC104882; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC137980; AAI37981.1; -; mRNA.
DR   CCDS; CCDS52683.1; -.
DR   RefSeq; NP_083704.1; NM_029428.1.
DR   AlphaFoldDB; Q9D5P4; -.
DR   SMR; Q9D5P4; -.
DR   STRING; 10090.ENSMUSP00000095964; -.
DR   PhosphoSitePlus; Q9D5P4; -.
DR   PaxDb; Q9D5P4; -.
DR   PRIDE; Q9D5P4; -.
DR   ProteomicsDB; 285759; -.
DR   ProteomicsDB; 334664; -.
DR   Antibodypedia; 30571; 70 antibodies from 20 providers.
DR   Ensembl; ENSMUST00000098361; ENSMUSP00000095964; ENSMUSG00000024266.
DR   GeneID; 75773; -.
DR   KEGG; mmu:75773; -.
DR   UCSC; uc009nqa.2; mouse.
DR   CTD; 161931; -.
DR   MGI; MGI:1923023; Adad2.
DR   VEuPathDB; HostDB:ENSMUSG00000024266; -.
DR   eggNOG; KOG2777; Eukaryota.
DR   GeneTree; ENSGT00940000161900; -.
DR   HOGENOM; CLU_005382_4_2_1; -.
DR   InParanoid; Q9D5P4; -.
DR   OMA; SCAGWLE; -.
DR   OrthoDB; 947117at2759; -.
DR   PhylomeDB; Q9D5P4; -.
DR   TreeFam; TF315806; -.
DR   BioGRID-ORCS; 75773; 5 hits in 72 CRISPR screens.
DR   PRO; PR:Q9D5P4; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; Q9D5P4; protein.
DR   Bgee; ENSMUSG00000024266; Expressed in spermatocyte and 30 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0003726; F:double-stranded RNA adenosine deaminase activity; IBA:GO_Central.
DR   GO; GO:0003725; F:double-stranded RNA binding; IBA:GO_Central.
DR   GO; GO:0008251; F:tRNA-specific adenosine deaminase activity; IBA:GO_Central.
DR   GO; GO:0006382; P:adenosine to inosine editing; IBA:GO_Central.
DR   GO; GO:0006396; P:RNA processing; IBA:GO_Central.
DR   GO; GO:0007286; P:spermatid development; IMP:UniProtKB.
DR   InterPro; IPR002466; A_deamin.
DR   InterPro; IPR014720; dsRBD_dom.
DR   Pfam; PF02137; A_deamin; 1.
DR   Pfam; PF00035; dsrm; 1.
DR   SMART; SM00552; ADEAMc; 1.
DR   SMART; SM00358; DSRM; 1.
DR   PROSITE; PS50141; A_DEAMIN_EDITASE; 1.
DR   PROSITE; PS50137; DS_RBD; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Differentiation; Nucleus; Reference proteome; RNA-binding;
KW   Spermatogenesis.
FT   CHAIN           1..561
FT                   /note="Adenosine deaminase domain-containing protein 2"
FT                   /id="PRO_0000309526"
FT   DOMAIN          90..159
FT                   /note="DRBM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   DOMAIN          239..560
FT                   /note="A to I editase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00240"
FT   REGION          1..53
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        331
FT                   /note="P -> L (in Ref. 1; BAB29697)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   561 AA;  59299 MW;  09F3E703E61F7C08 CRC64;
     MASVDEGGRR RPRLAASLQI SPGPWKPSGG QEPTEAGDAA PRTAEHGVAG AQEAHREACK
     ALGGSVLSPG PAGDFPGALH GLSMLPKDPP PAQAVALLTQ CMANLGVSLT FLEDQTAGPG
     SSFSVCADLD GLVCPAGTGS SKLEAKQQAA LSALQYIQKQ LERPEPLVTP RQPLLTSLSI
     ETILTHEQRC AAVVSAGLDR LLSESSPYQA CKGTVAAVIL EREVQGSIGH SKETYELVAL
     GTGSSSCAGW LEFSGRRLHD CHGLVIARRA LLRFFFRQLL LVTQGGPKGQ ERSVLTPQPG
     PGPPFALKPG VFLHLYVSNT PKGAAHDIYL PLASEDSVLH SPAFRLQAHV CGQLKPVSYV
     APALRDTHVG CLSASDKLAR WAILGLGGGL LAHFLPPLYA TSLVLADPCH DPSTLNRVIH
     SRPRLDSVLG SCLPCPYVRT TLHLFAGPLV APSDPGPSTC HSLSLNWSLG DPDIEVVDVA
     TGRVKTDSSV GPPSRLCKAA FLSAFRQVAR ALEKPQLLSL QTYEAAKAVP YREARQQLSL
     LLDQQGLGAW PSKPLVGKFR H