ID   DHE2_ACHKL              Reviewed;        1063 AA.
AC   P41755;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=NAD-specific glutamate dehydrogenase;
DE            Short=NAD-GDH;
DE            EC=1.4.1.2;
OS   Achlya klebsiana.
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae;
OC   Achlya.
OX   NCBI_TaxID=4767;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=8308022; DOI=10.1016/s0021-9258(17)41809-6;
RA   Lejohn H.B., Cameron L.E., Yang B., Rennie S.L.;
RT   "Molecular characterization of an NAD-specific glutamate dehydrogenase gene
RT   inducible by L-glutamine. Antisense gene pair arrangement with L-glutamine-
RT   inducible heat shock 70-like protein gene.";
RL   J. Biol. Chem. 269:4523-4531(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + NADH +
CC         NH4(+); Xref=Rhea:RHEA:15133, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.4.1.2;
CC   -!- ACTIVITY REGULATION: Allosterically activated by NADP(+).
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
CC       Highly divergent. {ECO:0000305}.
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DR   EMBL; U02505; AAA17563.1; -; Unassigned_DNA.
DR   PIR; A53164; A53164.
DR   AlphaFoldDB; P41755; -.
DR   GO; GO:0004352; F:glutamate dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR019651; Glutamate_DH_NAD-spec.
DR   Pfam; PF10712; NAD-GH; 1.
PE   3: Inferred from homology;
KW   Allosteric enzyme; NAD; Oxidoreductase.
FT   CHAIN           1..1063
FT                   /note="NAD-specific glutamate dehydrogenase"
FT                   /id="PRO_0000182731"
SQ   SEQUENCE   1063 AA;  118741 MW;  4EAC1E2C36674860 CRC64;
     MLLCIVLFLI FHILINQAGI VHVLYNASFV ASSSRFILFC EELFKATPQY WQWGNSIVQN
     GDASIASTLY LTMRTFIKHW KYTLLIMINQ SISYSCYSTN EASHLLFTNL PKVAPYTLGP
     LNFECCLLSS HHIRHIVLMF YGANIDHGLT KYELYTVCRP SSDEKLCNKI LNSPRLHTHN
     RYLYLTEVRI VGAFYLIKQV GARMVGLLVH LAFMVLQNLP KLIWPLDLVH VRQKGTCTAH
     IAYHPLAQKH FPPYFTHFYL NVTIYAVVKN ILDNISSSFT LQSNRVRPRP DVAGFDFFHR
     FDAFEQSLTY GGDTGITSSN EKVALCFEAT YTDIFFTVEI IDSTSVDSGA IMLPILVDAY
     TAVARNFCSV SMVISPLVTL VVYDIYTCSF IGLCTQRRTI NCTRILDAIS SVGNSHTFLP
     RYKSPRELTT RQPSGSSVIL LIFLLLGFEF FLFSGLVVVE PVNDVGDLVV NDLLVAFIDL
     ALELFVVEGV AEVVGVVFKT VLGFNADVVG FIFRLVLFSF LHHAFNIILG EATLVVGNGN
     LVFFTSRLFD GRHVQDTVGI NVEGDINLWN TTRHWRNTIE GELPEQVVVT GHRTLTFKHL
     NQHTRLVVSV GGESLRLLGW HSSVTLDEGS HDTTSSFQTK RERSDIKKQQ VLELFRRVVT
     AQNGSLDCGT ESNSFIRVDR LAWFLAVEEV RKQLLDLWDT GGTTDKDDFM DLALGELRVT
     EDLFNRFHSL AEVVTAHVFE TGTGDGGVEI NTIEERVDFN VSLGRRRKST LGTFTSGTKT
     AKGTLVLGHI LAVLALEFSG KVVDEAVIEI FTTQVGITSS SLDFEDTFFN GQKRHIEGTT
     TKIENENIAF TTLLVKTVGN GGTSRFVNDT KDVKTSNGTS ILGSLTLRVV EISWDGNDSV
     VNSSTNEGFS NFLHLDQNHR GNFFRLESLS FTLEFDGDLW LVTSTRGNLE WPVLNIGLSS
     WVVEFTTDQT LSIEHSVGRV HGNLVLSGIT NKTFAVSESN VRWGGTVTLI VGNNFNTIVL
     PDTDTRISRT EIDTDGSSLD TRHACSKFKN EVDSPPHLTS AFI