ID   DHE4_HEBCY              Reviewed;         450 AA.
AC   Q96UJ9;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=NADP-specific glutamate dehydrogenase;
DE            Short=NADP-GDH;
DE            EC=1.4.1.4;
DE   AltName: Full=NADP-dependent glutamate dehydrogenase;
OS   Hebeloma cylindrosporum.
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Hymenogastraceae; Hebeloma.
OX   NCBI_TaxID=76867;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=h1;
RA   Rodriguez-Pastrana B., Javelle A., Belleville R., Morel M., Botton B.,
RA   Jacob C., Chalot M., Brun A.;
RT   "Nucleotide sequence and expression of NADP(+)-GDH and GS from the
RT   ectomycorrhizal fungus Hebeloma cylindrosporum.";
RL   Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + NADPH +
CC         NH4(+); Xref=Rhea:RHEA:11612, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.4.1.4;
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
CC       {ECO:0000305}.
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DR   EMBL; AY052476; AAL06075.1; -; mRNA.
DR   AlphaFoldDB; Q96UJ9; -.
DR   SMR; Q96UJ9; -.
DR   BRENDA; 1.4.1.4; 7425.
DR   GO; GO:0004354; F:glutamate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006520; P:cellular amino acid metabolic process; IEA:InterPro.
DR   CDD; cd05313; NAD_bind_2_Glu_DH; 1.
DR   InterPro; IPR046346; Aminiacid_DH-like_N_sf.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DH.
DR   InterPro; IPR033524; Glu/Leu/Phe/Val_DH_AS.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DH_C.
DR   InterPro; IPR006097; Glu/Leu/Phe/Val_DH_dimer_dom.
DR   InterPro; IPR014362; Glu_DH.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR033922; NAD_bind_Glu_DH.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   Pfam; PF02812; ELFV_dehydrog_N; 1.
DR   PIRSF; PIRSF000185; Glu_DH; 1.
DR   PRINTS; PR00082; GLFDHDRGNASE.
DR   SMART; SM00839; ELFV_dehydrog; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF53223; SSF53223; 1.
DR   PROSITE; PS00074; GLFV_DEHYDROGENASE; 1.
PE   2: Evidence at transcript level;
KW   NADP; Oxidoreductase.
FT   CHAIN           1..450
FT                   /note="NADP-specific glutamate dehydrogenase"
FT                   /id="PRO_0000182788"
FT   ACT_SITE        111
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10011"
SQ   SEQUENCE   450 AA;  48356 MW;  3D0E79953F3E0746 CRC64;
     MVKPFEPEFQ QALDELTTSL QPFLDANPQY KKALEIVQVP ERVLQFRVVW EDDQGVAQVN
     HGFRVQYNSA LGPYKGGLRL HPSVNLSILK FLGFEQTFKN ALTGLSMGGG KGGSDFDPKG
     KSDSEIRRFC FSFMGELFRH IGSDTDVPAG DIGTGGREIG FLFGAYKKLR NEFTGMLTGK
     GLTWGGSFIR PEATGYGLIY FVEHMIAKAC PEYSLDKPST LVAISGSGNV AQFTALKVIE
     LGATVLSLSD SKGSLISDKG YTKEFIRKVA DLKLKGGSLA SLANEEGYTY HAGARPWTLI
     PTIHIALPGA TQNEVSAEEA EALLKAGVRI VAEGSNMGCT ADAIDIFESS RKAGPGGVWY
     APGKASNCGG VAVSGLEMAQ NSQRLAWTTQ EVDSKLKDIM AECYGICLTA GTKWSGEELT
     DEVLPSLLSG ANVAGFIKVA DAMKAQGDWW