ID   DHEA_NICPL              Reviewed;         411 AA.
AC   O04937;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Glutamate dehydrogenase A;
DE            Short=GDH A;
DE            EC=1.4.1.3;
GN   Name=GDHA;
OS   Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4092;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   PubMed=10353219; DOI=10.1093/oxfordjournals.pcp.a029547;
RA   Ficarelli A., Tassi F., Restivo F.M.;
RT   "Isolation and characterization of two cDNA clones encoding for glutamate
RT   dehydrogenase in Nicotiana plumbaginifolia.";
RL   Plant Cell Physiol. 40:339-342(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Leaf;
RA   Restivo F.M., Panizzi N., De Monte G., Ficarelli A.;
RT   "Genomic organisation of GDH genes in Nicotiana plumbaginifolia.";
RL   Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + NADH +
CC         NH4(+); Xref=Rhea:RHEA:15133, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.4.1.3;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + NADPH +
CC         NH4(+); Xref=Rhea:RHEA:11612, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.4.1.3;
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
CC       {ECO:0000305}.
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DR   EMBL; Y08292; CAA69600.1; -; mRNA.
DR   EMBL; AJ277949; CAB94836.1; -; Genomic_DNA.
DR   PIR; T16981; T16981.
DR   AlphaFoldDB; O04937; -.
DR   SMR; O04937; -.
DR   BRENDA; 1.4.1.3; 3640.
DR   GO; GO:0004352; F:glutamate dehydrogenase (NAD+) activity; IEA:RHEA.
DR   GO; GO:0004354; F:glutamate dehydrogenase (NADP+) activity; IEA:RHEA.
DR   GO; GO:0006520; P:cellular amino acid metabolic process; IEA:InterPro.
DR   CDD; cd01076; NAD_bind_1_Glu_DH; 1.
DR   InterPro; IPR046346; Aminiacid_DH-like_N_sf.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DH.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DH_C.
DR   InterPro; IPR006097; Glu/Leu/Phe/Val_DH_dimer_dom.
DR   InterPro; IPR014362; Glu_DH.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR033922; NAD_bind_Glu_DH.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   Pfam; PF02812; ELFV_dehydrog_N; 1.
DR   PIRSF; PIRSF000185; Glu_DH; 1.
DR   PRINTS; PR00082; GLFDHDRGNASE.
DR   SMART; SM00839; ELFV_dehydrog; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF53223; SSF53223; 1.
PE   2: Evidence at transcript level;
KW   NAD; Oxidoreductase.
FT   CHAIN           1..411
FT                   /note="Glutamate dehydrogenase A"
FT                   /id="PRO_0000182750"
FT   ACT_SITE        102
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   411 AA;  44796 MW;  5CCF132AF2103904 CRC64;
     MNALAATNRN FRQAARILGL DSKLEKSLLI PFREIKVECT IPKDDGTLVS YVGFRVQHDN
     ARGPMKGGIR YHPEVDLDEV NALAQLMTWK TAVADIPYGG AKGGIGCKPK DLSKSELERL
     TRVFTQKIHD LIGINTDVPA PDMGTNAQTM AWILDEYSKF HGHSPAIVTG KPIDLGGSLG
     REAATGRGVV YATEALLAEY GKNIKDLTFA IQGFGNVGAW AAKLIHERGG KVIAVSDITG
     AVKNPNGLDI PALLNHKEAT GKLIDFSGGD VMNSDEVLTH ECDVLIPCAL GGVLNRENAD
     NVKAKFIIEA ANHPTDPEAD EILCKKGIVI LPDIYANAGG VTVSYFEWVQ NIQGFMWDEE
     KVNRELRKYM TKAFHNLKNM CQSHNCSLRM GAFTLGVNRV ARATTLRGWE A