ID   DHK2_STRVN              Reviewed;         249 AA.
AC   P16543;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Granaticin polyketide synthase putative ketoacyl reductase 2;
DE            EC=1.3.1.-;
DE   AltName: Full=ORF6;
GN   Name=gra-orf6;
OS   Streptomyces violaceoruber.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1935;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Tu22;
RX   PubMed=2583128; DOI=10.1002/j.1460-2075.1989.tb08413.x;
RA   Sherman D.H., Malpartida F., Bibb M.J., Kieser H.M., Bibb M.J.,
RA   Hopwood D.A.;
RT   "Structure and deduced function of the granaticin-producing polyketide
RT   synthase gene cluster of Streptomyces violaceoruber Tu22.";
RL   EMBO J. 8:2717-2725(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Tu22;
RX   PubMed=9831526; DOI=10.1016/s1074-5521(98)90292-7;
RA   Ichinose K., Bedford D.J., Tornus D., Bechthold A., Bibb M.J., Revill W.P.,
RA   Floss H.G., Hopwood D.A.;
RT   "The granaticin biosynthetic gene cluster of Streptomyces violaceoruber
RT   Tu22: sequence analysis and expression in a heterologous host.";
RL   Chem. Biol. 5:647-659(1998).
CC   -!- PATHWAY: Antibiotic biosynthesis; granaticin biosynthesis.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; X16300; CAA34367.1; -; Genomic_DNA.
DR   EMBL; X16144; CAA34262.1; -; Genomic_DNA.
DR   EMBL; AJ011500; CAA09651.1; -; Genomic_DNA.
DR   PIR; S05398; S05398.
DR   AlphaFoldDB; P16543; -.
DR   SMR; P16543; -.
DR   UniPathway; UPA00175; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; NAD; Oxidoreductase.
FT   CHAIN           1..249
FT                   /note="Granaticin polyketide synthase putative ketoacyl
FT                   reductase 2"
FT                   /id="PRO_0000054633"
FT   ACT_SITE        157
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         12..36
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         144
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   249 AA;  25977 MW;  922832B88FE34A8B CRC64;
     MATDAPEAPV ALVTGSSSGI GQTVAQRLAA EGYRVVVNSA RSVEDGEKTA AALPDALYVR
     ADVSEEADAR RLVDTAVEHY GRLDVLVNNA GRTRAIPHAD LAAATPEVWR EILGLNVIGT
     WQTTVAAMPH LARSGNGSVV NVSSIAGSRP AGSSIPYAVS NGGHRAQTRL LANTVGPAVR
     VNAVAPGLIE TPWTQNSDFF APIAEHVRQT TPLRRTGRPE DVAEAVLGLV RATYTTGQVL
     LVDGGAHLL