ID   DHKR_STRCM              Reviewed;         261 AA.
AC   P41177;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Monensin polyketide synthase putative ketoacyl reductase;
DE            EC=1.3.1.-;
DE   AltName: Full=ORF5;
OS   Streptomyces cinnamonensis.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces virginiae group.
OX   NCBI_TaxID=1900;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A3823.5;
RX   PubMed=1508151; DOI=10.1007/bf00283846;
RA   Arrowsmith T.J., Malpartida F., Sherman D.H., Birch A., Hopwood D.A.,
RA   Robinson J.A.;
RT   "Characterisation of actI-homologous DNA encoding polyketide synthase genes
RT   from the monensin producer Streptomyces cinnamonensis.";
RL   Mol. Gen. Genet. 234:254-264(1992).
CC   -!- PATHWAY: Antifungal biosynthesis; monensin biosynthesis.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; Z11511; CAA77599.1; -; Genomic_DNA.
DR   PIR; S25079; S25079.
DR   AlphaFoldDB; P41177; -.
DR   SMR; P41177; -.
DR   UniPathway; UPA00178; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; NAD; Oxidoreductase.
FT   CHAIN           1..261
FT                   /note="Monensin polyketide synthase putative ketoacyl
FT                   reductase"
FT                   /id="PRO_0000054634"
FT   ACT_SITE        157
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         10..34
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         144
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   261 AA;  27081 MW;  095743C37FF63CCC CRC64;
     MTQSTSRVAL VTGATSGIGL ATARLLAAQG HLVFLGARTE SDVIATVKAL RNDGLEAEGQ
     VLDVRDGASV TAFVQAAVDR YGRIDVLVNN AGRSGGGVTA DLTDELWDDV IDTNLNSVFR
     MTRAVLTTGG MRTRERGRII NVASTAGKQG VVLGAPYSAS KHGVVGFTKA LGNELAPTGI
     TVNAVCPGYV ETPMAQRVRQ GYAAAYDTTE EAILTKFQAK IPLGRYSTPE EVAGLIGYLA
     SDTAASITSQ ALNVCGGLGN F