ID   DHLO_AGRT4              Reviewed;         358 AA.
AC   P0A394; P00385;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Protein ocs;
DE   Includes:
DE     RecName: Full=D-lysopine dehydrogenase;
DE              EC=1.5.1.16;
DE     AltName: Full=Lysopine synthase;
DE   Includes:
DE     RecName: Full=D-octopine dehydrogenase;
DE              EC=1.5.1.11;
DE     AltName: Full=Octopine synthase;
GN   Name=ocs;
OS   Agrobacterium tumefaciens (strain Ach5).
OG   Plasmid pTiAch5.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176298;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7153687;
RA   de Greve H., Dhaese P., Seurinck J., Lemmers M., van Montagu M., Schell J.;
RT   "Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti
RT   plasmid-encoded octopine synthase gene.";
RL   J. Mol. Appl. Genet. 1:499-511(1982).
CC   -!- FUNCTION: Reductive condensation of pyruvate and arginine, lysine,
CC       histidine, or octopine to form octopine, lysopine, histopine, or
CC       octopinic acid, respectively. NADPH is the preferred cofactor, but NADH
CC       can also be used.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-octopine + H2O + NAD(+) = H(+) + L-arginine + NADH +
CC         pyruvate; Xref=Rhea:RHEA:16285, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:32682, ChEBI:CHEBI:57520,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.11;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-lysopine + H2O + NADP(+) = H(+) + L-lysine + NADPH +
CC         pyruvate; Xref=Rhea:RHEA:17625, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:32551, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58058, ChEBI:CHEBI:58349; EC=1.5.1.16;
CC   -!- SUBUNIT: Monomer.
CC   -!- SIMILARITY: Belongs to the lysopine/nopaline/octopine/opine/vitopine
CC       dehydrogenases family. {ECO:0000305}.
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DR   EMBL; V00088; CAA23428.1; -; Genomic_DNA.
DR   PIR; A00399; DEAGLT.
DR   RefSeq; NP_059680.1; NC_002377.1.
DR   AlphaFoldDB; P0A394; -.
DR   SMR; P0A394; -.
DR   GO; GO:0047827; F:D-lysopine dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047830; F:D-octopine dehydrogenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR003421; Opine_DH.
DR   Pfam; PF02317; Octopine_DH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Crown gall tumor; NAD; NADP; Oxidoreductase; Plasmid.
FT   CHAIN           1..358
FT                   /note="Protein ocs"
FT                   /id="PRO_0000179978"
SQ   SEQUENCE   358 AA;  38797 MW;  C57D977E4EC4D803 CRC64;
     MAKVAILGAG NVALTLAGDL ARRLGQVSSI WAPISNRNSF NSVRSLGSLE LVGPDYGGDF
     QPQLEDDLET AISGAAFIFL TVPTMGQQGI LCELANFNLS SSVLVALPGS ATSLACKQTL
     TPAFAPIAVI EATTSPYACR RVNAQVLMLS VKRTFEVAST QALSEEVRGG FEILFPNRLQ
     WYQNPASIFF SNTNPVAHPA GILAAKDTIE QGISPIPKFY RKFVPQAITR VTAIDEERLT
     IVNALGLESE TDFAYCKKWY GGHASNAREF YETFEGYADI ETPRNMNHRY LSEDVKHILV
     LWVEIAEVIG VQVPEMKSVV QEASDVLNED LSHTGRGLSS LNLEGSNANA IVRALNGV