ID   DHLO_AGRVI              Reviewed;         358 AA.
AC   P94210;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Protein ocs;
DE   Includes:
DE     RecName: Full=D-lysopine dehydrogenase;
DE              EC=1.5.1.16;
DE     AltName: Full=Lysopine synthase;
DE   Includes:
DE     RecName: Full=D-octopine dehydrogenase;
DE              EC=1.5.1.11;
DE     AltName: Full=Octopine synthase;
GN   Name=ocs;
OS   Agrobacterium vitis (Rhizobium vitis).
OG   Plasmid pTiTM4.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=373;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CG474;
RA   Otten L., de Ruffray P.;
RL   Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Reductive condensation of pyruvate and arginine, lysine,
CC       histidine, or octopine to form octopine, lysopine, histopine, or
CC       octopinic acid, respectively. NADPH is the preferred cofactor, but NADH
CC       can also be used.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-octopine + H2O + NAD(+) = H(+) + L-arginine + NADH +
CC         pyruvate; Xref=Rhea:RHEA:16285, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:32682, ChEBI:CHEBI:57520,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.11;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-lysopine + H2O + NADP(+) = H(+) + L-lysine + NADPH +
CC         pyruvate; Xref=Rhea:RHEA:17625, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:32551, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58058, ChEBI:CHEBI:58349; EC=1.5.1.16;
CC   -!- SIMILARITY: Belongs to the lysopine/nopaline/octopine/opine/vitopine
CC       dehydrogenases family. {ECO:0000305}.
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DR   EMBL; U83987; AAB41877.1; -; Genomic_DNA.
DR   RefSeq; WP_060718528.1; NZ_WPHX01000055.1.
DR   AlphaFoldDB; P94210; -.
DR   SMR; P94210; -.
DR   GeneID; 60685092; -.
DR   GO; GO:0047827; F:D-lysopine dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047830; F:D-octopine dehydrogenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR003421; Opine_DH.
DR   Pfam; PF02317; Octopine_DH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Crown gall tumor; NAD; NADP; Oxidoreductase; Plasmid.
FT   CHAIN           1..358
FT                   /note="Protein ocs"
FT                   /id="PRO_0000179980"
SQ   SEQUENCE   358 AA;  38860 MW;  83F7D389BD16498F CRC64;
     MAKVAILGAG NLALTLAGDL ARRLGQTPSI WAPISNRSSF NDVRCLGSLE LVGPDYGGDF
     QPRLEDDLGT AISGAAFIFL TVPTLGQQGI LRELAKFNLS NSVLVALPGS ATSLACKQTL
     VPTFAPIAVI ESTTSPYACR RVKARVLMLG VKATFEVATT QPLSEEVKGR FEVLFPNPPQ
     WYQHPASIFF SNTNPVAHPA GILAARDSIE QGILPVPKFY RQFVPQAITR VIAIDEERLS
     IVDALGLESE TDFSYSKKWY GGHACNAREF YETYEGYAEI ETPKTMNHRY LTEDVKHILV
     LWVEIAEAIG VQVPEMKSVV QEASDVLNEN LLRTGRGLSS LNLGGANANA IVRALNGV