ID   DHMA2_MYCTO             Reviewed;         286 AA.
AC   P9WMS0; L0TAR1; P64303; Q50600;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Haloalkane dehalogenase 2;
DE            EC=3.8.1.5;
GN   Name=dhmA2; OrderedLocusNames=MT1881;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC       halogenated aliphatic compounds, leading to the formation of the
CC       corresponding primary alcohols, halide ions and protons. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC         H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE000516; AAK46153.1; -; Genomic_DNA.
DR   PIR; B70722; B70722.
DR   RefSeq; WP_003409254.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WMS0; -.
DR   SMR; P9WMS0; -.
DR   EnsemblBacteria; AAK46153; AAK46153; MT1881.
DR   GeneID; 45425806; -.
DR   KEGG; mtc:MT1881; -.
DR   PATRIC; fig|83331.31.peg.2025; -.
DR   HOGENOM; CLU_020336_13_3_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   HAMAP; MF_01230; Haloalk_dehal_type1; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   InterPro; IPR023489; Haloalkane_dehalogenase_1.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..286
FT                   /note="Haloalkane dehalogenase 2"
FT                   /id="PRO_0000427253"
FT   DOMAIN          35..134
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        109
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        238
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        267
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   286 AA;  32151 MW;  A4080E76B3E7B37E CRC64;
     MSIDFTPDPQ LYPFESRWFD SSRGRIHYVD EGTGPPILLC HGNPTWSFLY RDIIVALRDR
     FRCVAPDYLG FGLSERPSGF GYQIDEHARV IGEFVDHLGL DRYLSMGQDW GGPISMAVAV
     ERADRVRGVV LGNTWFWPAD TLAMKAFSRV MSSPPVQYAI LRRNFFVERL IPAGTEHRPS
     SAVMAHYRAV QPNAAARRGV AEMPKQILAA RPLLARLARE VPATLGTKPT LLIWGMKDVA
     FRPKTIIPRL SATFPDHVLV ELPNAKHFIQ EDAPDRIAAA IIERFG