DHMA_CAUSK
ID DHMA_CAUSK Reviewed; 302 AA.
AC B0SY51;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=Haloalkane dehalogenase {ECO:0000255|HAMAP-Rule:MF_01230};
DE EC=3.8.1.5 {ECO:0000255|HAMAP-Rule:MF_01230};
GN Name=dhmA {ECO:0000255|HAMAP-Rule:MF_01230}; OrderedLocusNames=Caul_2671;
OS Caulobacter sp. (strain K31).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter; unclassified Caulobacter.
OX NCBI_TaxID=366602;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K31;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T.,
RA Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Kim E., Stephens C., Richardson P.;
RT "Complete sequence of chromosome of Caulobacter sp. K31.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC halogenated aliphatic compounds, leading to the formation of the
CC corresponding primary alcohols, halide ions and protons.
CC {ECO:0000255|HAMAP-Rule:MF_01230}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01230};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01230}.
CC -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01230}.
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DR EMBL; CP000927; ABZ71798.1; -; Genomic_DNA.
DR RefSeq; WP_012286701.1; NC_010338.1.
DR AlphaFoldDB; B0SY51; -.
DR SMR; B0SY51; -.
DR STRING; 366602.Caul_2671; -.
DR ESTHER; causk-dhma; Haloalkane_dehalogenase-HLD1.
DR EnsemblBacteria; ABZ71798; ABZ71798; Caul_2671.
DR KEGG; cak:Caul_2671; -.
DR eggNOG; COG0596; Bacteria.
DR HOGENOM; CLU_020336_13_3_5; -.
DR OMA; TLFCQDW; -.
DR OrthoDB; 1890883at2; -.
DR GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.1820; -; 1.
DR HAMAP; MF_01230; Haloalk_dehal_type1; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR000639; Epox_hydrolase-like.
DR InterPro; IPR023489; Haloalkane_dehalogenase_1.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR PRINTS; PR00111; ABHYDROLASE.
DR PRINTS; PR00412; EPOXHYDRLASE.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 3: Inferred from homology;
KW Hydrolase.
FT CHAIN 1..302
FT /note="Haloalkane dehalogenase"
FT /id="PRO_1000085715"
FT DOMAIN 48..150
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT ACT_SITE 123
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
FT ACT_SITE 249
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
FT ACT_SITE 278
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
SQ SEQUENCE 302 AA; 32910 MW; 15A0985A717230E1 CRC64;
MQVLRTPESR FAGLADWPFA PHYAEITDAG GARLRLHYVD EGPRDGAPVL LMHGEPSWAY
LYRKIIPALA ARGHRVIAPD LIGFGRSDKP AARGDYTYER HVAWMSAWLE GLDLRGLTLF
CQDWGGLIGL RLVAAFPERF AGLVIANTGL PTGAGMTDGF KAWLDFSQNV PEMPIGLIVN
MGTGRDLTPA EIAAYDAPFP DETYKEGARQ FPMLVPVTPE HASVAENLAA WKVLEHFPGP
VVTAFSDGDP VTRGGEAIFQ SRMPGALGQP HRTLRGGHFL QEDCPDDIVD IVDAVARPES
QS
