DHMA_CAUVC
ID DHMA_CAUVC Reviewed; 302 AA.
AC Q9A919;
DT 22-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Haloalkane dehalogenase {ECO:0000255|HAMAP-Rule:MF_01230};
DE EC=3.8.1.5 {ECO:0000255|HAMAP-Rule:MF_01230};
GN Name=dhmA {ECO:0000255|HAMAP-Rule:MF_01230}; OrderedLocusNames=CC_1175;
OS Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=190650;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=11259647; DOI=10.1073/pnas.061029298;
RA Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E.,
RA Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R.,
RA Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B.,
RA DeBoy R.T., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F.,
RA Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R.,
RA Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O.,
RA Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M.;
RT "Complete genome sequence of Caulobacter crescentus.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001).
CC -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC halogenated aliphatic compounds, leading to the formation of the
CC corresponding primary alcohols, halide ions and protons.
CC {ECO:0000255|HAMAP-Rule:MF_01230}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01230};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01230}.
CC -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01230}.
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DR EMBL; AE005673; AAK23159.1; -; Genomic_DNA.
DR PIR; C87395; C87395.
DR RefSeq; NP_419991.1; NC_002696.2.
DR RefSeq; WP_010919059.1; NC_002696.2.
DR PDB; 5ESR; X-ray; 1.48 A; A=1-302.
DR PDBsum; 5ESR; -.
DR AlphaFoldDB; Q9A919; -.
DR SMR; Q9A919; -.
DR STRING; 190650.CC_1175; -.
DR ESTHER; caucr-CC1175; Haloalkane_dehalogenase-HLD1.
DR EnsemblBacteria; AAK23159; AAK23159; CC_1175.
DR KEGG; ccr:CC_1175; -.
DR PATRIC; fig|190650.5.peg.1198; -.
DR eggNOG; COG0596; Bacteria.
DR HOGENOM; CLU_020336_13_3_5; -.
DR OMA; TLFCQDW; -.
DR BioCyc; CAULO:CC1175-MON; -.
DR BRENDA; 3.8.1.5; 1218.
DR Proteomes; UP000001816; Chromosome.
DR GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.1820; -; 1.
DR HAMAP; MF_01230; Haloalk_dehal_type1; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR000639; Epox_hydrolase-like.
DR InterPro; IPR023489; Haloalkane_dehalogenase_1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR PRINTS; PR00111; ABHYDROLASE.
DR PRINTS; PR00412; EPOXHYDRLASE.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Reference proteome.
FT CHAIN 1..302
FT /note="Haloalkane dehalogenase"
FT /id="PRO_0000216765"
FT DOMAIN 48..152
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT ACT_SITE 123
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
FT ACT_SITE 249
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
FT ACT_SITE 278
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
FT HELIX 8..11
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 22..27
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 33..42
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 48..52
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 59..62
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 63..71
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 89..92
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 93..95
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 98..111
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 116..122
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 124..135
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 137..139
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 140..147
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 158..169
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 175..181
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 189..196
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 202..205
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 206..214
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 224..234
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 241..243
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 246..248
FT /evidence="ECO:0007829|PDB:5ESR"
FT TURN 250..252
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 253..255
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 256..262
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 264..266
FT /evidence="ECO:0007829|PDB:5ESR"
FT STRAND 274..279
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 280..283
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 285..298
FT /evidence="ECO:0007829|PDB:5ESR"
FT HELIX 299..301
FT /evidence="ECO:0007829|PDB:5ESR"
SQ SEQUENCE 302 AA; 33155 MW; B0B8C27EE8D9A97C CRC64;
MDVLRTPDER FEGLADWSFA PHYTEVTDAD GTALRIHHVD EGPKDQRPIL LMHGEPSWAY
LYRKVIAELV AKGHRVVAPD LVGFGRSDKP AKRTDYTYER HVAWMSAWLE QNDLKDIVLF
CQDWGGLIGL RLVAAFPERF SAVVVSNTGL PIGVGKSEGF EAWLNFSQNT PELPVGFILN
GGTARDLSDA ERSAYDAPFP DESYKEGARI FPALVPITPE HASVEENKAA WAVLETFDKP
FVTAFSDADP ITRGGEAMFL ARVPGTKNVA HTTLKGGHFV QEDSPVEIAA LLDGLVAGLP
QA
