ID   DHMA_MYCPA              Reviewed;         301 AA.
AC   Q73Y99;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Haloalkane dehalogenase {ECO:0000255|HAMAP-Rule:MF_01230};
DE            EC=3.8.1.5 {ECO:0000255|HAMAP-Rule:MF_01230};
GN   Name=dhmA {ECO:0000255|HAMAP-Rule:MF_01230}; OrderedLocusNames=MAP_2057;
OS   Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10)
OS   (Mycobacterium paratuberculosis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium avium complex (MAC).
OX   NCBI_TaxID=262316;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-968 / K-10;
RX   PubMed=16116077; DOI=10.1073/pnas.0505662102;
RA   Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N.,
RA   Kanjilal S., Kapur V.;
RT   "The complete genome sequence of Mycobacterium avium subspecies
RT   paratuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005).
CC   -!- FUNCTION: Catalyzes hydrolytic cleavage of carbon-halogen bonds in
CC       halogenated aliphatic compounds, leading to the formation of the
CC       corresponding primary alcohols, halide ions and protons.
CC       {ECO:0000255|HAMAP-Rule:MF_01230}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-haloalkane + H2O = a halide anion + a primary alcohol +
CC         H(+); Xref=Rhea:RHEA:19081, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15734, ChEBI:CHEBI:16042, ChEBI:CHEBI:18060; EC=3.8.1.5;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01230};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01230}.
CC   -!- SIMILARITY: Belongs to the haloalkane dehalogenase family. Type 1
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01230}.
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DR   EMBL; AE016958; AAS04374.1; -; Genomic_DNA.
DR   RefSeq; WP_003872427.1; NC_002944.2.
DR   AlphaFoldDB; Q73Y99; -.
DR   SMR; Q73Y99; -.
DR   STRING; 262316.MAP_2057; -.
DR   ESTHER; mycav-DHMA; Haloalkane_dehalogenase-HLD1.
DR   EnsemblBacteria; AAS04374; AAS04374; MAP_2057.
DR   GeneID; 66693599; -.
DR   KEGG; mpa:MAP_2057; -.
DR   eggNOG; COG0596; Bacteria.
DR   HOGENOM; CLU_020336_13_3_11; -.
DR   OMA; IQEDSGP; -.
DR   BRENDA; 3.8.1.5; 3492.
DR   Proteomes; UP000000580; Chromosome.
DR   GO; GO:0018786; F:haloalkane dehalogenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   HAMAP; MF_01230; Haloalk_dehal_type1; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   InterPro; IPR023489; Haloalkane_dehalogenase_1.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..301
FT                   /note="Haloalkane dehalogenase"
FT                   /id="PRO_1000066841"
FT   DOMAIN          47..284
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        123
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
FT   ACT_SITE        250
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
FT   ACT_SITE        279
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01230"
SQ   SEQUENCE   301 AA;  33842 MW;  AE2731C504BAAAE6 CRC64;
     MHVLRTPDSR FENLEDYPFV AHYLDVTARD TRPLRMHYLD EGPIDGPPIV LLHGEPTWSY
     LYRTMITPLT DAGNRVLAPD LIGFGRSDKP SRIEDYSYQR HVDWVVSWFE HLNLSDVTLF
     VQDWGSLIGL RIAAEQPDRV GRLVVANGFL PTAQRRTPPA FYAWRAFARY SPVLPAGRIV
     SVGTVRRVSS KVRAGYDAPF PDKTYQAGAR AFPQLVPTSP ADPAIPANRK AWEALGRWEK
     PFLAIFGARD PILGHADSPL IKHIPGAAGQ PHARINASHF IQEDRGPELA ERILSWQQAL
     L