ID   DHOM_METGL              Reviewed;         435 AA.
AC   P37143;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Homoserine dehydrogenase;
DE            Short=HDH;
DE            EC=1.1.1.3;
GN   Name=hom;
OS   Methylobacillus glycogenes.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Methylophilaceae; Methylobacillus.
OX   NCBI_TaxID=406;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 21276 / NCIMB 12105;
RX   PubMed=8117070; DOI=10.1128/aem.60.1.111-119.1994;
RA   Motoyama H., Maki K., Anazawa H., Ishino S., Teshiba S.;
RT   "Cloning and nucleotide sequences of the homoserine dehydrogenase genes
RT   (hom) and the threonine synthase genes (thrC) of the Gram-negative obligate
RT   methylotroph Methylobacillus glycogenes.";
RL   Appl. Environ. Microbiol. 60:111-119(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde +
CC         NADPH; Xref=Rhea:RHEA:15761, ChEBI:CHEBI:15378, ChEBI:CHEBI:57476,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:537519; EC=1.1.1.3;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde +
CC         NADH; Xref=Rhea:RHEA:15757, ChEBI:CHEBI:15378, ChEBI:CHEBI:57476,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:537519; EC=1.1.1.3;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC       pathway; L-homoserine from L-aspartate: step 3/3.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine
CC       from L-aspartate: step 3/5.
CC   -!- SIMILARITY: Belongs to the homoserine dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; D14070; BAA40414.1; -; Genomic_DNA.
DR   AlphaFoldDB; P37143; -.
DR   SMR; P37143; -.
DR   UniPathway; UPA00050; UER00063.
DR   UniPathway; UPA00051; UER00465.
DR   GO; GO:0004412; F:homoserine dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR001342; HDH_cat.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00742; Homoserine_dh; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Isoleucine biosynthesis; Methionine biosynthesis; NADP; Oxidoreductase;
KW   Threonine biosynthesis.
FT   CHAIN           1..435
FT                   /note="Homoserine dehydrogenase"
FT                   /id="PRO_0000066698"
FT   REGION          255..274
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          377..402
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        204
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   BINDING         9..16
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         105
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         190
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   435 AA;  48227 MW;  58468B0E7A81ACAB CRC64;
     MKPINVGLLG IGTVGGGTYT VLTRNQEGNR APCRPPNCHH ARRDRNLELA RKVTGGQIDV
     TDDAFAVVVR PRHRHRGRID RRIHHRARTG YEGDRERQAR VAANKALMLA WQRDFRRVAQ
     QKGVIVAFEA AVAGGIPIIK AVAAGAGRQP SSRVLRAIIN GTTNFILSEM REKGLAFADV
     LKEAQPRLCE ATRPSTSRAW TAHKLMILAA IGFGIPMQFD KAYVEGISKL DALDIRYAEE
     LGYRQAAWHA PSAPARGVEL ARAPDPDPRE TPDRQCQRRH NAVLVSDACR SHLVYRPDAC
     RRHASAVVAD IVDGTRTAYH RRAPAVPHLL SSPTSSWTCR FAIGEVSSAY YLRLRAVDKP
     GRDGHVTRIL ATGSLHRCDD SEGNRRQAER RRSGRHHHPD HVTVEKNMDD AIVAIEALPA
     ISGSVTRLRM EELSR