DHPS_NEIMB
ID DHPS_NEIMB Reviewed; 285 AA.
AC Q51161; Q51157; Q51158; Q51159; Q51160; Q57214; Q60395;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Dihydropteroate synthase;
DE Short=DHPS;
DE EC=2.5.1.15;
DE AltName: Full=Dihydropteroate pyrophosphorylase;
GN Name=folP; Synonyms=dhpS; OrderedLocusNames=NMB1691;
OS Neisseria meningitidis serogroup B (strain MC58).
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC Neisseria.
OX NCBI_TaxID=122586;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=952 / Serogroup B / Serotype NT, BT490 / Serogroup B / Serotype 15,
RC and MO035 / Serogroup B / Serotype 15;
RX PubMed=1400191; DOI=10.1128/jb.174.20.6386-6393.1992;
RA Raadstroem P., Fermer C., Kristiansen B.-E., Jenkins A., Skoeld O.,
RA Swedberg G.;
RT "Transformational exchanges in the dihydropteroate synthase gene of
RT Neisseria meningitidis: a novel mechanism for acquisition of sulfonamide
RT resistance.";
RL J. Bacteriol. 174:6386-6393(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=3976 / Serogroup B / Serotype NT;
RX PubMed=7642493; DOI=10.1128/jb.177.16.4669-4675.1995;
RA Fermer C., Kristiansen B.E., Skoeld O., Swedberg G.;
RT "Sulfonamide resistance in Neisseria meningitidis as defined by site-
RT directed mutagenesis could have its origin in other species.";
RL J. Bacteriol. 177:4669-4675(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MC58;
RX PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA Moxon E.R., Rappuoli R., Venter J.C.;
RT "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT MC58.";
RL Science 287:1809-1815(2000).
CC -!- FUNCTION: Catalyzes the condensation of para-aminobenzoate (pABA) with
CC 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-
CC dihydropteroate (H2Pte), the immediate precursor of folate derivatives.
CC {ECO:0000250|UniProtKB:P0AC13}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate =
CC 7,8-dihydropteroate + diphosphate; Xref=Rhea:RHEA:19949,
CC ChEBI:CHEBI:17836, ChEBI:CHEBI:17839, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:72950; EC=2.5.1.15;
CC Evidence={ECO:0000250|UniProtKB:P0AC13};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:P0AC13};
CC -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-
CC dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-
CC dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the DHPS family. {ECO:0000305}.
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DR EMBL; X68062; CAA48199.1; -; Genomic_DNA.
DR EMBL; X68066; CAA48203.1; -; Genomic_DNA.
DR EMBL; X68069; CAA48206.1; -; Genomic_DNA.
DR EMBL; X87405; CAA60855.1; -; Genomic_DNA.
DR EMBL; AE002098; AAF42039.1; -; Genomic_DNA.
DR PIR; A57423; A57423.
DR PIR; S25611; S25611.
DR PIR; S65832; S65832.
DR PIR; S65839; S65839.
DR RefSeq; NP_274695.1; NC_003112.2.
DR RefSeq; WP_002224358.1; NC_003112.2.
DR AlphaFoldDB; Q51161; -.
DR SMR; Q51161; -.
DR STRING; 122586.NMB1691; -.
DR PaxDb; Q51161; -.
DR EnsemblBacteria; AAF42039; AAF42039; NMB1691.
DR KEGG; nme:NMB1691; -.
DR PATRIC; fig|122586.8.peg.2174; -.
DR HOGENOM; CLU_008023_0_2_4; -.
DR OMA; FSIDTYH; -.
DR UniPathway; UPA00077; UER00156.
DR Proteomes; UP000000425; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004156; F:dihydropteroate synthase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0046656; P:folic acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IBA:GO_Central.
DR CDD; cd00739; DHPS; 1.
DR Gene3D; 3.20.20.20; -; 1.
DR InterPro; IPR045031; DHP_synth.
DR InterPro; IPR006390; DHP_synth_dom.
DR InterPro; IPR011005; Dihydropteroate_synth-like.
DR InterPro; IPR000489; Pterin-binding_dom.
DR PANTHER; PTHR20941; PTHR20941; 1.
DR Pfam; PF00809; Pterin_bind; 1.
DR SUPFAM; SSF51717; SSF51717; 1.
DR TIGRFAMs; TIGR01496; DHPS; 1.
DR PROSITE; PS00792; DHPS_1; 1.
DR PROSITE; PS50972; PTERIN_BINDING; 1.
PE 3: Inferred from homology;
KW Folate biosynthesis; Magnesium; Metal-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..285
FT /note="Dihydropteroate synthase"
FT /id="PRO_0000168218"
FT DOMAIN 18..276
FT /note="Pterin-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00334"
FT BINDING 25
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:P9WND1"
FT BINDING 66
FT /ligand="(7,8-dihydropterin-6-yl)methyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:72950"
FT /evidence="ECO:0000250|UniProtKB:P0AC13"
FT BINDING 99
FT /ligand="(7,8-dihydropterin-6-yl)methyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:72950"
FT /evidence="ECO:0000250|UniProtKB:P0AC13"
FT BINDING 119
FT /ligand="(7,8-dihydropterin-6-yl)methyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:72950"
FT /evidence="ECO:0000250|UniProtKB:P0AC13"
FT BINDING 190
FT /ligand="(7,8-dihydropterin-6-yl)methyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:72950"
FT /evidence="ECO:0000250|UniProtKB:P0AC13"
FT BINDING 229
FT /ligand="(7,8-dihydropterin-6-yl)methyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:72950"
FT /evidence="ECO:0000250|UniProtKB:P0AC13"
FT BINDING 264..266
FT /ligand="(7,8-dihydropterin-6-yl)methyl diphosphate"
FT /ligand_id="ChEBI:CHEBI:72950"
FT /evidence="ECO:0000250|UniProtKB:P0AC13"
FT VARIANT 2..4
FT /note="ARH -> VGC (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 31
FT /note="F -> L (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 36
FT /note="V -> A (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 42
FT /note="Q -> R (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 50
FT /note="Q -> R (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 68
FT /note="S -> P (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 69
FT /note="G -> C (in strain: BT490 / Serogroup B / Serotype
FT 15)"
FT VARIANT 84
FT /note="P -> S (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 96
FT /note="I -> V (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 102
FT /note="R -> H (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 105
FT /note="I -> V (in strain: 952 / Serogroup B / Serotype NT
FT and 3976 / Serogroup B / Serotype NT)"
FT VARIANT 125
FT /note="N -> T (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 130
FT /note="V -> L (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 135
FT /note="R -> C (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 137
FT /note="A -> T (in strain: BT490 / Serogroup B / Serotype
FT 15)"
FT VARIANT 147
FT /note="Q -> R (in strain: BT490 / Serogroup B / Serotype
FT 15)"
FT VARIANT 151
FT /note="K -> E (in strain: BT490 / Serogroup B / Serotype 15
FT and 952 / Serogroup B / Serotype NT)"
FT VARIANT 152
FT /note="T -> N (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 172
FT /note="A -> T (in strain: 952 / Serogroup B / Serotype NT)"
FT VARIANT 174
FT /note="S -> A (in strain: BT490 / Serogroup B / Serotype 15
FT and 3976 / Serogroup B / Serotype NT)"
FT VARIANT 175..178
FT /note="AECI -> ETCV (in strain: 952 / Serogroup B /
FT Serotype NT)"
FT VARIANT 188
FT /note="I -> T (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 194..195
FT /note="Missing (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 196
FT /note="G -> C (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 200
FT /note="P -> T (in strain: 952 / Serogroup B / Serotype NT
FT and 3976 / Serogroup B / Serotype NT)"
FT VARIANT 206
FT /note="A -> T (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 208
FT /note="M -> V (in strain: BT490 / Serogroup B / Serotype
FT 15)"
FT VARIANT 220
FT /note="F -> Y (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 230
FT /note="S -> R (in strain: 952 / Serogroup B / Serotype NT
FT and BT490 / Serogroup B / Serotype 15)"
FT VARIANT 231
FT /note="T -> M (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 232
FT /note="I -> V (in strain: BT490 / Serogroup B / Serotype
FT 15)"
FT VARIANT 239
FT /note="A -> S (in strain: BT490 / Serogroup B / Serotype
FT 15)"
FT VARIANT 239
FT /note="A -> T (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 240
FT /note="N -> D (in strain: BT490 / Serogroup B / Serotype
FT 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B
FT / Serotype NT)"
FT VARIANT 243
FT /note="E -> A (in strain: BT490 / Serogroup B / Serotype 15
FT and 3976 / Serogroup B / Serotype NT)"
FT VARIANT 245
FT /note="V -> G (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 253
FT /note="L -> V (in strain: BT490 / Serogroup B / Serotype
FT 15)"
FT VARIANT 255
FT /note="S -> A (in strain: BT490 / Serogroup B / Serotype 15
FT and 3976 / Serogroup B / Serotype NT)"
FT VARIANT 261
FT /note="Q -> K (in strain: 3976 / Serogroup B / Serotype
FT NT)"
FT VARIANT 277
FT /note="V -> A (in strain: 3976 / Serogroup B / Serotype
FT NT)"
SQ SEQUENCE 285 AA; 30313 MW; C56518F61CF98A4E CRC64;
MARHVWQAGR FEIGLDKPKI MGIVNLTPDS FSDGGVYSQN AQTALAHAEQ LLKEGADILD
IGGESTRSGA DYVSPEEEWA RVEPVLAEVA GWGVPISLDT RRTVIMEKAL ALGGIDIIND
VAALNDEGAV ELLARQADTG ICLMHMQGLP KTMQINPKYQ DVVGEVARYL KARSAECIAA
GIAPQRIILD PGFGSGFGKP LQHNIALMRH LPELMAETGF PLLIGVSRKS TIGELTGEAN
AAERVHGSVA AALASVARGA QIVRVHDVKA TADALKVWEA LGINL
