ID   ADAT1_CHICK             Reviewed;         503 AA.
AC   Q5ZI16;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=tRNA-specific adenosine deaminase 1;
DE            EC=3.5.4.34;
DE   AltName: Full=tRNA-specific adenosine-37 deaminase;
GN   Name=ADAT1; ORFNames=RCJMB04_31g18;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA   Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA   Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:R6.1-R6.9(2005).
CC   -!- FUNCTION: Specifically deaminates adenosine-37 to inosine in tRNA-Ala.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(37) in tRNA(Ala) + H(+) + H2O = inosine(37) in
CC         tRNA(Ala) + NH4(+); Xref=Rhea:RHEA:50968, Rhea:RHEA-COMP:12855,
CC         Rhea:RHEA-COMP:12856, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:74411, ChEBI:CHEBI:82852; EC=3.5.4.34;
CC   -!- COFACTOR:
CC       Name=1D-myo-inositol hexakisphosphate; Xref=ChEBI:CHEBI:58130;
CC         Evidence={ECO:0000250};
CC       Note=Binds 1 myo-inositol hexakisphosphate (IP6) per subunit.
CC       {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the ADAT1 family. {ECO:0000305}.
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DR   EMBL; AJ720968; CAG32627.1; -; mRNA.
DR   RefSeq; NP_001012797.1; NM_001012779.1.
DR   AlphaFoldDB; Q5ZI16; -.
DR   SMR; Q5ZI16; -.
DR   STRING; 9031.ENSGALP00000001334; -.
DR   GeneID; 415886; -.
DR   KEGG; gga:415886; -.
DR   CTD; 23536; -.
DR   VEuPathDB; HostDB:geneid_415886; -.
DR   eggNOG; KOG2777; Eukaryota.
DR   InParanoid; Q5ZI16; -.
DR   OrthoDB; 947117at2759; -.
DR   PhylomeDB; Q5ZI16; -.
DR   PRO; PR:Q5ZI16; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR   GO; GO:0008251; F:tRNA-specific adenosine deaminase activity; ISS:UniProtKB.
DR   GO; GO:0008033; P:tRNA processing; ISS:UniProtKB.
DR   InterPro; IPR002466; A_deamin.
DR   Pfam; PF02137; A_deamin; 1.
DR   SMART; SM00552; ADEAMc; 1.
DR   PROSITE; PS50141; A_DEAMIN_EDITASE; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Metal-binding; Reference proteome; tRNA processing; Zinc.
FT   CHAIN           1..503
FT                   /note="tRNA-specific adenosine deaminase 1"
FT                   /id="PRO_0000287649"
FT   DOMAIN          63..502
FT                   /note="A to I editase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00240"
FT   ACT_SITE        89
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00240"
FT   BINDING         87
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00240"
FT   BINDING         94
FT                   /ligand="1D-myo-inositol hexakisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58130"
FT                   /evidence="ECO:0000250"
FT   BINDING         142
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00240"
FT   BINDING         300
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00240"
FT   BINDING         303
FT                   /ligand="1D-myo-inositol hexakisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58130"
FT                   /evidence="ECO:0000250"
FT   BINDING         306
FT                   /ligand="1D-myo-inositol hexakisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58130"
FT                   /evidence="ECO:0000250"
FT   BINDING         436
FT                   /ligand="1D-myo-inositol hexakisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58130"
FT                   /evidence="ECO:0000250"
FT   BINDING         471
FT                   /ligand="1D-myo-inositol hexakisphosphate"
FT                   /ligand_id="ChEBI:CHEBI:58130"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   503 AA;  55901 MW;  A3CD6832D0A18C31 CRC64;
     MWTADEIAEL CYEHYRSRLP KQGKPDPSRE WTSLAAVVKV ESAANEAGSA VLGTLQVAKE
     VVALGTGTKC IGLNKMRKTG DVLNDSHAEV VAKRSFQRYL LHQMRLATSY QQCSIFIPGT
     ETGKWKLKPN IIFIFFCSHT PCGDASIIPI RETENHLSKS VDGHDIAGQS VLCSSSNCDH
     RGPEDKRKSE KMASSHMIKR MKNADGGFFS TITEDMAVQQ VFAKPEGNVN PECCESSEEM
     QAANKETNAG KLKAVGVYRT GAKFVPGELS DTLIPGIEYH CVGLLRVKPG RGDRTCSMSC
     SDKLARWNVL GCQGALLMHF LQYPVYLSAV IVGKCPYSQE AMQRAVIERC RHISLLPDGF
     LTQEVQLLQS DLQFEHSRQA IQEGQTSSKR KLVPCSAAIS WSAVPEGPLD VTSDGFRQGT
     TKKGIGSPQS RSKICKVELF HEFQKLVTSI SKENLPDTLR MKTLETYWDY KEAALNYQEA
     WKALRSQALL GWIKNAQEYL LFM