ID   DHSS_ANACY              Reviewed;         383 AA.
AC   P16421;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Soluble hydrogenase 42 kDa subunit;
DE            EC=1.12.-.-;
DE   AltName: Full=Tritium exchange subunit;
OS   Anabaena cylindrica.
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Anabaena.
OX   NCBI_TaxID=1165;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-13.
RX   PubMed=2129525; DOI=10.1111/j.1432-1033.1990.tb15297.x;
RA   Ewart G.D., Reed K.C., Smith G.D.;
RT   "Soluble hydrogenase of Anabaena cylindrica. Cloning and sequencing of a
RT   potential gene encoding the tritium exchange subunit.";
RL   Eur. J. Biochem. 187:215-223(1990).
CC   -!- FUNCTION: Soluble hydrogenase catalyzes both production and consumption
CC       of hydrogen from suitable artificial electron donors or acceptors. This
CC       subunit catalyzes the tritium-exchange activity.
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000305}.
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DR   EMBL; X17482; CAA35518.1; -; Genomic_DNA.
DR   PIR; S07767; S07767.
DR   AlphaFoldDB; P16421; -.
DR   SMR; P16421; -.
DR   OMA; KNWLPIM; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR020578; Aminotrans_V_PyrdxlP_BS.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   InterPro; IPR024169; SP_NH2Trfase/AEP_transaminase.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   PIRSF; PIRSF000524; SPT; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   PROSITE; PS00595; AA_TRANSFER_CLASS_5; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Oxidoreductase; Pyridoxal phosphate.
FT   CHAIN           1..383
FT                   /note="Soluble hydrogenase 42 kDa subunit"
FT                   /id="PRO_0000150242"
FT   MOD_RES         194
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   383 AA;  41155 MW;  B79F5F54A7CABFAF CRC64;
     MDDKLMLMIP GPTPVPEAAL LALAKHPIGH RTSEFSNMMG EVTQNLKWLH QTESDVLMLN
     VSGTGAVEAG MINFLSPGDR ILVGSNGKFG ERWVEVGQAF GLNVEAITAE WGQPLDPDKF
     AQKLQADTNK EIKAVIITHS ETSTGVINDL VAINSHVKEH GQALIIVDAV TSLGAYNVPV
     DALGLDVVAS GSQKGYMIPP GLGFVSVSPK AWEAYKTAKL PKYYLDLGKY RKATAKNTTP
     FTPPVNLMVA LHTTLGMMKK EGLESIFTRH ERQKNATRAA MKALNLPLFA ADECASPAIT
     AVATPGMEAD KIRSLMKKRF DIALAGGQDH LSNKIFRVGH LGFVSDRDIL SCIASLEVVL
     LELGHENFNS GAGVAAAARV FSN