ID   DHSS_SYNP1              Reviewed;         384 AA.
AC   P14776;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Soluble hydrogenase, small subunit;
DE            EC=1.12.-.-;
DE   AltName: Full=Tritium exchange subunit;
OS   Synechococcus sp. (strain PCC 6716).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC   unclassified Synechococcus.
OX   NCBI_TaxID=32048;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2513553;
RA   van der Oost J., van Walraven H.S., Bogerd J., Smit A.B., Ewart G.D.,
RA   Smith G.D.;
RT   "Nucleotide sequence of the gene proposed to encode the small subunit of
RT   the soluble hydrogenase of the thermophilic unicellular cyanobacterium
RT   Synechococcus PCC 6716.";
RL   Nucleic Acids Res. 17:10098-10098(1989).
CC   -!- FUNCTION: Soluble hydrogenase catalyzes both production and consumption
CC       of hydrogen from suitable artificial electron donors or acceptors. This
CC       subunit catalyzes the tritium-exchange activity.
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X16658; CAA34644.1; -; Genomic_DNA.
DR   PIR; S06919; HQYCSS.
DR   AlphaFoldDB; P14776; -.
DR   SMR; P14776; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR020578; Aminotrans_V_PyrdxlP_BS.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   InterPro; IPR024169; SP_NH2Trfase/AEP_transaminase.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   PIRSF; PIRSF000524; SPT; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   PROSITE; PS00595; AA_TRANSFER_CLASS_5; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Oxidoreductase; Pyridoxal phosphate.
FT   CHAIN           1..384
FT                   /note="Soluble hydrogenase, small subunit"
FT                   /id="PRO_0000150243"
FT   MOD_RES         194
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   384 AA;  40876 MW;  89BF4A278F7E9AC2 CRC64;
     MQDKAMLMIP GPTPVPESVL LSLGKHPIGH RSGEFSQIMA AMTAGIKWLH QTQNEVLILA
     ASGTGAMEAG IINFLSAGDR VVVGCNGKFG DRWGEVCDAY GLTTERISAP WGQPLNPDDF
     KAVLDGHRQK PSKAVIVTHS ETSTGVINDL EAINRHVKAH GQALIIVDAV TSLGAVSVPI
     DEWGLDVVGS GSQKGYMIPP GLAFVSVSPK AWEAYKTATL PKFYLDLGKY RKDAAKHTTP
     FTPPVNLFFA LKTALEMMQA EGLEAIFQRH QRLMQATRAA MKALNLPLYA ADSCASPAIT
     AVAPQGVEAE NIRSLMKKRF DIALAGGQDH LKGQIFRIGH LGFVGDRDIL AAVSALEAVL
     AELGYTNFTP GAGVAAASRV LSTA