DHU1_ARATH
ID DHU1_ARATH Reviewed; 783 AA.
AC Q8GYY7; Q9SYZ3;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Protein DWD HYPERSENSITIVE TO UV-B 1 {ECO:0000303|PubMed:25193399, ECO:0000303|PubMed:28735869};
GN Name=DHU1 {ECO:0000303|PubMed:25193399, ECO:0000303|PubMed:28735869};
GN OrderedLocusNames=At4g34280 {ECO:0000312|Araport:AT4G34280};
GN ORFNames=F10M10.50 {ECO:0000312|EMBL:AEE86354.1},
GN F10M10_50 {ECO:0000312|EMBL:AEE86354.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH DDB1A, AND INDUCTION BY
RP UV-B.
RC STRAIN=cv. Columbia;
RX PubMed=25193399; DOI=10.1007/s11103-014-0247-0;
RA Kim S.-H., Kim H., Seo K.-I., Kim S.-H., Chung S., Huang X., Yang P.,
RA Deng X.W., Lee J.-H.;
RT "DWD HYPERSENSITIVE TO UV-B 1 is negatively involved in UV-B mediated
RT cellular responses in Arabidopsis.";
RL Plant Mol. Biol. 86:571-583(2014).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH COP1 AND RUP1.
RC STRAIN=cv. Columbia;
RX PubMed=28735869; DOI=10.1016/j.bbrc.2017.07.110;
RA Kim S.-H., Kim H., Chung S., Lee J.-H.;
RT "DHU1 negatively regulates UV-B signaling via its direct interaction with
RT COP1 and RUP1.";
RL Biochem. Biophys. Res. Commun. 491:285-290(2017).
CC -!- FUNCTION: May act as a substrate receptor of a CUL4-RING E3 ubiquitin-
CC protein ligase (CRL4) complex involved in the negative regulation of
CC cellular responses to ultraviolet-B (UV-B) illumination, likely in
CC coordination with RUP1 (PubMed:25193399, PubMed:28735869). Interacts
CC with COP1 and probably prevents the formation of active UVR8-COP1
CC complex, thus avoiding UVR8-COP1-mediated positive regulation of UV-B
CC responses (PubMed:28735869). {ECO:0000269|PubMed:25193399,
CC ECO:0000269|PubMed:28735869}.
CC -!- SUBUNIT: Interacts directly with DDB1A (PubMed:25193399). Binds to COP1
CC and RUP1 (PubMed:28735869). {ECO:0000269|PubMed:25193399,
CC ECO:0000269|PubMed:28735869}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- INDUCTION: Accumulates in response to ultraviolet-B (UV-B)
CC illumination. {ECO:0000269|PubMed:25193399}.
CC -!- DISRUPTION PHENOTYPE: Hypersensitivity to ultraviolet-B (UV-B)
CC illumination leading to short hypocotyls under UV-B; the sensitivity to
CC UV-B is alleviated by the disruption of UVR8 and requires the presence
CC of COP1 and HY5 (PubMed:25193399, PubMed:28735869). The double mutants
CC dhu1-1 cop1-6 and dhu1-1 hy5-215 phenotypes resemble that of single
CC mutants cop1-6 and hy5-215, respectively (PubMed:28735869). The UV-B
CC responsiveness of the double mutant dhu1-1 rup1-1 is similar to that of
CC the single mutants dhu1-1 and rup1-1 (PubMed:28735869).
CC {ECO:0000269|PubMed:25193399, ECO:0000269|PubMed:28735869}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB36705.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80145.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL035521; CAB36705.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161585; CAB80145.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86354.1; -; Genomic_DNA.
DR EMBL; AK117309; BAC41980.1; -; mRNA.
DR EMBL; BT005961; AAO64896.1; -; mRNA.
DR PIR; T04774; T04774.
DR RefSeq; NP_195154.2; NM_119593.4.
DR AlphaFoldDB; Q8GYY7; -.
DR SMR; Q8GYY7; -.
DR STRING; 3702.AT4G34280.1; -.
DR PaxDb; Q8GYY7; -.
DR PRIDE; Q8GYY7; -.
DR ProteomicsDB; 191457; -.
DR EnsemblPlants; AT4G34280.1; AT4G34280.1; AT4G34280.
DR GeneID; 829578; -.
DR Gramene; AT4G34280.1; AT4G34280.1; AT4G34280.
DR KEGG; ath:AT4G34280; -.
DR Araport; AT4G34280; -.
DR TAIR; locus:2116179; AT4G34280.
DR eggNOG; KOG0531; Eukaryota.
DR HOGENOM; CLU_012003_0_0_1; -.
DR InParanoid; Q8GYY7; -.
DR OMA; YMINSLP; -.
DR OrthoDB; 238457at2759; -.
DR PhylomeDB; Q8GYY7; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8GYY7; baseline and differential.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; IPI:TAIR.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0071493; P:cellular response to UV-B; IMP:TAIR.
DR GO; GO:0010224; P:response to UV-B; IEP:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR046377; DHU1.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR003603; U2A'_phosphoprotein32A_C.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR47201; PTHR47201; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00446; LRRcap; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..783
FT /note="Protein DWD HYPERSENSITIVE TO UV-B 1"
FT /id="PRO_0000454923"
FT REPEAT 145..198
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 212..256
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 439..480
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 485..525
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 538..577
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 581..621
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 625..664
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 666..710
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT MOTIF 382..389
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
SQ SEQUENCE 783 AA; 88171 MW; 1BBB2B4F937F87BF CRC64;
MATLTEIATL EEKYIELCKM HGILPNTAIL SAFFEAEVKK SRNQRCIMNL YVDRVKYDDY
LPLLELCNEI NTSEVQGIDL FVRSACSLED HYALPLIRSV NQKLRVVHLH DSFGKNFWQD
VFFQGLSCKV LNVRSMHIHK LNIVGEFTQL HTLILDKNRI VGFGEDCFSC MPKLTYLSMC
DTLVSDLWTS AAALLKLPSL KELRFQIWIS CSDSSPLNSE SSPSSSTKDD INTFIESDPP
VEADMWDVAE QMDPSLPVEE TLHSMDFSYK IPEQDDLDSH VSVSAGLNGE VLMREKVRRG
KMPYQPKDVS PVDTFTRQFG NVGLKYISSK ASPICSEKHY RMYMINSLPK LQVLDNLAIR
KSDRDKAIET YSANFEDLPY KRKKESVVRV LENREKRSSK GKSQNSYKRS LCAAKMGSPA
SPLLHSLPFL SSRIQQEDDN SRLCPRQFEY HPLDPSLMVF GTLDGEVVVL NHESGKIFRY
IPSNGSQSTI LGLCWLKIYP SMVIAGSANG SLKLYDIQKA SSTVTTSSHS TSGSVTFDEF
DQLTSVHANS TDQLFLASGY SKDVALYDIG RGTRLQVFAN MHQEHINVVK FSNHSPFLFA
TSSFDKDVKL WDLRQEPSRP CYTASSTKGN VMVCFSPDDR YLLASAVDNE VRQLLTVDGR
LHLNFEIVPR VSSMNYTRSY YMNGNDYIIS GSCDENVIRV CCAQTGRRLR DVTLEGNGSD
FSMMYVQSLR GDPFRDFNMS VLAAYARSSS LSEIVKVNLL ASRDSTAEES HGLRSYPSSS
MGG
