DHX15_CAEEL
ID DHX15_CAEEL Reviewed; 739 AA.
AC Q20875;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Pre-mRNA-splicing factor ATP-dependent RNA helicase ddx-15 {ECO:0000250|UniProtKB:O43143};
DE EC=3.6.4.13 {ECO:0000250|UniProtKB:O43143};
DE AltName: Full=DEAH box protein 15 {ECO:0000250|UniProtKB:O43143};
GN Name=ddx-15 {ECO:0000312|WormBase:F56D2.6a};
GN ORFNames=F56D2.6 {ECO:0000312|WormBase:F56D2.6a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Pre-mRNA processing factor involved in disassembly of
CC spliceosomes after the release of mature mRNA.
CC {ECO:0000250|UniProtKB:O43143}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000250|UniProtKB:O43143};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O35286}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC DDX15/PRP43 sub-subfamily. {ECO:0000305}.
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DR EMBL; FO080358; CCD63138.1; -; Genomic_DNA.
DR PIR; T16482; T16482.
DR RefSeq; NP_741147.1; NM_171130.4.
DR AlphaFoldDB; Q20875; -.
DR SMR; Q20875; -.
DR BioGRID; 41000; 16.
DR STRING; 6239.F56D2.6a; -.
DR iPTMnet; Q20875; -.
DR EPD; Q20875; -.
DR PaxDb; Q20875; -.
DR PeptideAtlas; Q20875; -.
DR PRIDE; Q20875; -.
DR EnsemblMetazoa; F56D2.6a.1; F56D2.6a.1; WBGene00018967.
DR GeneID; 175771; -.
DR KEGG; cel:CELE_F56D2.6; -.
DR UCSC; F56D2.6a; c. elegans.
DR CTD; 175771; -.
DR WormBase; F56D2.6a; CE01334; WBGene00018967; ddx-15.
DR eggNOG; KOG0925; Eukaryota.
DR GeneTree; ENSGT00940000155800; -.
DR InParanoid; Q20875; -.
DR OMA; DHDLKRY; -.
DR OrthoDB; 354219at2759; -.
DR PhylomeDB; Q20875; -.
DR Reactome; R-CEL-72163; mRNA Splicing - Major Pathway.
DR PRO; PR:Q20875; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00018967; Expressed in embryo and 4 other tissues.
DR ExpressionAtlas; Q20875; baseline and differential.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; ISS:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR CDD; cd17973; DEXHc_DHX15; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044756; DHX15_DEXHc.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; mRNA processing; mRNA splicing;
KW Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..739
FT /note="Pre-mRNA-splicing factor ATP-dependent RNA helicase
FT ddx-15"
FT /evidence="ECO:0000305"
FT /id="PRO_0000055142"
FT DOMAIN 86..257
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 282..462
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 204..207
FT /note="DEAH box"
FT COMPBIAS 1..18
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..37
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 99..106
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 739 AA; 84389 MW; A8E9FBDCFE22F40F CRC64;
MSSRHRLDLD GSGRGDRRRS PNRRSRSRSR SPHRRSSPDR KRQIGAVGNM KIQINPYNNQ
PFSNRYWAIW EKRSQLPVWE YKEKFMELLR NNQCITLVGE TGSGKTTQIP QWAVEFMKQQ
QQGQPPGQAR LVACTQPRRV AAMSVATRVA EEMDVVLGQE VGYSIRFEDC ISERTVLKYC
TDGMLLREAM NSPLLDKYKV LILDEAHERT LATDILMGLI KEIVRNRADI KVVIMSATLD
AGKFQRYFED CPLLSVPGRT FPVEIFFTPN AEKDYLEAAI RTVIQIHMVE EVEGDILLFL
TGQEEIEEAC KRIDREIQAL GADAGALSCI PLYSTLPPAA QQRIFEPAPP NRPNGAISRK
CVISTNIAET SLTIDGVVFV IDPGFSKQKV YNPRIRVESL LVCPISKASA MQRAGRAGRT
KPGKCFRLYT ETAYGSEMQD QTYPEILRSN LGSVVLQLKK LGTEDLVHFD FMDPPAPETL
MRALELLNYL QAINDDGELT ELGSLMAEFP LDPQLAKMLI TSTELNCSNE ILSITAMLSV
PQCWVRPNEM RTEADEAKAR FAHIDGDHLT LLNVYHSFKQ NQEDPQWCYD NFINYRTMKT
ADTVRTQLSR VMDKYNLRRV STDFKSRDYY LNIRKALVAG FFMQVAHLER SGHYVTVKDN
QLVNLHPSTV LDHKPEWALY NEFVLTTKNF IRTVTDVRPE WLLQIAPQYY DLDNFPDGDT
KRKLTTVMQT LQRNAGRGY
