ID   ADAT2_BOVIN             Reviewed;         191 AA.
AC   Q5E9J7;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=tRNA-specific adenosine deaminase 2;
DE            EC=3.5.4.33 {ECO:0000305};
DE   AltName: Full=Deaminase domain-containing protein 1;
DE   AltName: Full=tRNA-specific adenosine-34 deaminase subunit ADAT2;
GN   Name=DEADC1; Synonyms=ADAT2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
CC   -!- FUNCTION: Probably participates in deamination of adenosine-34 to
CC       inosine in many tRNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA +
CC         NH4(+); Xref=Rhea:RHEA:43168, Rhea:RHEA-COMP:10373, Rhea:RHEA-
CC         COMP:10374, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:74411, ChEBI:CHEBI:82852; EC=3.5.4.33;
CC         Evidence={ECO:0000305};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC       family. ADAT2 subfamily. {ECO:0000305}.
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DR   EMBL; BT020923; AAX08940.1; -; mRNA.
DR   RefSeq; NP_001029952.1; NM_001034780.1.
DR   AlphaFoldDB; Q5E9J7; -.
DR   SMR; Q5E9J7; -.
DR   STRING; 9913.ENSBTAP00000037541; -.
DR   PaxDb; Q5E9J7; -.
DR   Ensembl; ENSBTAT00000037717; ENSBTAP00000037541; ENSBTAG00000026519.
DR   GeneID; 615418; -.
DR   KEGG; bta:615418; -.
DR   CTD; 134637; -.
DR   VEuPathDB; HostDB:ENSBTAG00000026519; -.
DR   VGNC; VGNC:25638; ADAT2.
DR   eggNOG; KOG1018; Eukaryota.
DR   GeneTree; ENSGT00390000000280; -.
DR   HOGENOM; CLU_025810_8_1_1; -.
DR   InParanoid; Q5E9J7; -.
DR   OMA; PCQMCAG; -.
DR   OrthoDB; 1616309at2759; -.
DR   TreeFam; TF313782; -.
DR   Proteomes; UP000009136; Chromosome 9.
DR   Bgee; ENSBTAG00000026519; Expressed in semen and 103 other tissues.
DR   GO; GO:0052717; F:tRNA-specific adenosine-34 deaminase activity; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0002100; P:tRNA wobble adenosine to inosine editing; IBA:GO_Central.
DR   HAMAP; MF_00972; tRNA_aden_deaminase; 1.
DR   InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd.
DR   InterPro; IPR002125; CMP_dCMP_dom.
DR   InterPro; IPR016193; Cytidine_deaminase-like.
DR   InterPro; IPR028883; tRNA_aden_deaminase.
DR   Pfam; PF14437; MafB19-deam; 1.
DR   SUPFAM; SSF53927; SSF53927; 1.
DR   PROSITE; PS00903; CYT_DCMP_DEAMINASES_1; 1.
DR   PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Metal-binding; Reference proteome; tRNA processing; Zinc.
FT   CHAIN           1..191
FT                   /note="tRNA-specific adenosine deaminase 2"
FT                   /id="PRO_0000287652"
FT   DOMAIN          20..145
FT                   /note="CMP/dCMP-type deaminase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01083"
FT   ACT_SITE        73
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         71
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         107
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         110
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   191 AA;  21127 MW;  82C3835B676A0037 CRC64;
     MEAKAGPTAA TDGAYSVSAE ETEKWMEQAM QMAKDALDNT EVPVGCLMVY NNEVVGKGRN
     EVNQTKNATR HAEMVAIDQA LDWCRRRGRS PSEVFEHTVL YVTVEPCIMC AAALRLMRIP
     LVVYGCQNER FGGCGSVLDI ASADLPSTGK PFQCTPGYRA EEAVEMLKTF YKQENPNAPK
     SKVRKKECHK S