DHX16_PIG
ID DHX16_PIG Reviewed; 1045 AA.
AC Q767K6;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16;
DE EC=3.6.4.13;
DE AltName: Full=DEAH-box protein 16;
GN Name=DHX16; Synonyms=DDX16;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Large white;
RX PubMed=14673549; DOI=10.1007/s00251-003-0627-0;
RA Shigenari A., Ando A., Renard C., Chardon P., Shiina T., Kulski J.K.,
RA Yasue H., Inoko H.;
RT "Nucleotide sequencing analysis of the swine 433-kb genomic segment located
RT between the non-classical and classical SLA class I gene clusters.";
RL Immunogenetics 55:695-705(2004).
CC -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC spliceosome. Contributes to pre-mRNA splicing after spliceosome
CC formation and prior to the first transesterification reaction.
CC {ECO:0000250|UniProtKB:O60231}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: Component of pre-catalytic spliceosome complexes. Interacts
CC with GPKOW. {ECO:0000250|UniProtKB:O60231}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O60231}. Nucleus,
CC nucleoplasm {ECO:0000250|UniProtKB:O60231}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC DDX16/PRP8 sub-subfamily. {ECO:0000305}.
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DR EMBL; AB113356; BAD08431.1; -; Genomic_DNA.
DR EMBL; AB113357; BAD08443.1; -; Genomic_DNA.
DR RefSeq; NP_001116578.1; NM_001123106.1.
DR AlphaFoldDB; Q767K6; -.
DR SMR; Q767K6; -.
DR STRING; 9823.ENSSSCP00000001456; -.
DR PaxDb; Q767K6; -.
DR PeptideAtlas; Q767K6; -.
DR PRIDE; Q767K6; -.
DR Ensembl; ENSSSCT00000084319; ENSSSCP00000065266; ENSSSCG00000001376.
DR Ensembl; ENSSSCT00045068351; ENSSSCP00045048624; ENSSSCG00045039260.
DR GeneID; 100144456; -.
DR KEGG; ssc:100144456; -.
DR CTD; 8449; -.
DR VGNC; VGNC:87287; DHX16.
DR eggNOG; KOG0923; Eukaryota.
DR GeneTree; ENSGT00940000158480; -.
DR InParanoid; Q767K6; -.
DR OMA; PHYHKKK; -.
DR OrthoDB; 354219at2759; -.
DR Proteomes; UP000008227; Chromosome 7.
DR Proteomes; UP000314985; Unplaced.
DR Bgee; ENSSSCG00000001376; Expressed in oocyte and 43 other tissues.
DR ExpressionAtlas; Q767K6; baseline.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; mRNA processing; mRNA splicing;
KW Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Spliceosome.
FT CHAIN 1..1045
FT /note="Pre-mRNA-splicing factor ATP-dependent RNA helicase
FT DHX16"
FT /id="PRO_0000402828"
FT DOMAIN 413..577
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 602..775
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 101..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1026..1045
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 524..527
FT /note="DEAH box"
FT COMPBIAS 144..211
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 426..433
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT MOD_RES 103
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O60231"
FT MOD_RES 106
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O60231"
FT MOD_RES 107
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O60231"
FT MOD_RES 164
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O60231"
FT MOD_RES 716
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O60231"
SQ SEQUENCE 1045 AA; 119648 MW; 8317FD5E383562CE CRC64;
MATPAGLERW VQDELHSVLG LSERHVAQFL IGTAQRCASA EEFVQRLRDT DTLDLSGPAR
DFALKLWNKV PRKAVAEKPA RAAEREARAL LEKNRSYRLL EDSEESSEEA VGRAGSSLQK
KRKKRKHLRK KRQEEEEEEE EEEVPEKGKK TTGGNKPQTE KPESEDEWER TERERLQDLE
ERDAFAERVR QRDKDRTRNV LERSDKKAYE EAQKRLKMAE EDRKAMVPEL RKKSRREYLA
KREREKLEDL EAELADEEFL FGDVELSRHE RRELKYKRRV RDLAREYRAA GEQEKLEATN
RYHMPEETRG QPTRAVDLVE EESGAPGEEQ RRWEEARLGA ASLKFGARDA ASQEPKYQLV
LEEEETIEFV RATQLQGDEE PAAPPAPTQA QQKESIQAVR RSLPVFPFRE ELLAAVANHQ
ILIIEGETGS GKTTQIPQYL FEEGYTQKGM KIACTQPRRV AAMSVAARVA REMGVKLGNE
VGYSIRFEDC TSERTVLRYM TDGMLLREFL SEPDLASYSV VMVDEAHERT LHTDILFGLI
KDVARFRPEL KVLVASATLD TARFSTFFDD APVFRIPGRR FPVDIFYTKA PEADYLEACV
VSVLQIHVTQ PPGDILVFLT GQEEIEAACE MLQDRCRRLG SKIRELLVLP IYANLPSDMQ
ARIFQPTPPG ARKVVVATNI AETSLTIEGI IYVLDPGFCK QKSYNPRTGM ESLTVTPCSK
ASANQRAGRA GRVAAGKCFR LYTAWAYQHE LEETTVPEIQ RTSLGNVVLL LKSLGIHDLM
HFDFLDPPPY ETLLLALEQL YALGALNHLG ELTTSGRKMA ELPVDPMLSK MILASEKYSC
SEEILTVAAM LSVNNSIFYR PKDKVVHADN ARVNFFLPGG DHLVLLNVYT QWAESGYSSQ
WCYENFVQFR SMRRARDVRE QLEGLLERVE VGLSSCQGDY IRVRKAITAG YFYHTARLTR
SGYRTVKQQQ TVFIHPNSSL FEEQPRWLLY HELVLTTKEF MRQVLEIESS WLLEVAPHYY
KAKELEDPHA KKMPKKTGKT REELG
