ID   DHX32_XENLA             Reviewed;         748 AA.
AC   Q5XH12;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32;
DE            EC=3.6.4.13;
GN   Name=dhx32;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Mitochondrion
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC084264; AAH84264.1; -; mRNA.
DR   RefSeq; NP_001088262.1; NM_001094793.1.
DR   AlphaFoldDB; Q5XH12; -.
DR   SMR; Q5XH12; -.
DR   GeneID; 495093; -.
DR   KEGG; xla:495093; -.
DR   CTD; 495093; -.
DR   Xenbase; XB-GENE-982116; dhx32.L.
DR   OrthoDB; 354219at2759; -.
DR   Proteomes; UP000186698; Chromosome 7L.
DR   Bgee; 495093; Expressed in gastrula and 18 other tissues.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF07717; OB_NTP_bind; 1.
DR   SMART; SM00847; HA2; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Mitochondrion; Nucleotide-binding;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..748
FT                   /note="Putative pre-mRNA-splicing factor ATP-dependent RNA
FT                   helicase DHX32"
FT                   /id="PRO_0000292665"
FT   DOMAIN          74..240
FT                   /note="Helicase ATP-binding"
FT   DOMAIN          263..439
FT                   /note="Helicase C-terminal"
FT   REGION          706..748
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        706..720
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        721..740
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         87..94
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   748 AA;  85112 MW;  91BCD35A67B36305 CRC64;
     MALSLEAYEQ DIDELISDEK SCCLDVTDSS DVEGDELNDL ELNPFDGLPY SSRFYKLLKE
     RETLPIWKIK YDFLEHLAHN QIVVVSAGPK SGKSSQIPQW CAEYCLAGHY QHGAVVCTQA
     HKQTAVWLAM RVADEMDVNI GHEVGYIVPF ENCCTSETIL RYCTDEMLRR EMMSNPLLSS
     YGVVIIDDVY ERFVSTDVLL SFLKVIAVSR PELKVVIITC PSLSGTLVSY YGNAPLVEAE
     NTHSVESVYT TSLPRDYFHS ALRLLFEIHH TKEKGDIVVF LACEEEIKRA YEHIKQEALH
     MNPELGELMP IALYPHQSIS DYIPYEELDD NSKNPKRKVV LTTSLGESLI WMKNIFFVID
     VGIEKRKVYN TRIRAESLVT QPISKVRAKM RKHILSSSSE GKLFCLYPED FAHEMKPFLT
     AKVEECNLIS MVLFLKRMDI AGLAHCDFIN RPDPESLMQA LEDLDYLAAL DNDGNLSEFG
     IIMSEFPLDP QLSKSILAAC EFDCVDEMLT LAAMVTAPNC FIDLPPEAKE LDLIGKGKFF
     HPEGDHFTLI NIYNEYEQMK RNNASQYDVE KWCQNHCLSF VALEMARAIR NELLDIMRRI
     ELPLTGPAFG SDENVTNIKK SLLSGYFMQI ARDVDGLGNY IMLTHKQVGQ LHPDSGFCNS
     AKVPEWVLFH EFSVSERSCI RIVSEISPNL FMEFVPPYYF SNLPPSETKD LLQQDQTPDT
     PPTEEPREEE PLHEANDEGT AEQRCIIQ