ID   DHX35_PONAB             Reviewed;         720 AA.
AC   Q5RBD4;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Probable ATP-dependent RNA helicase DHX35;
DE            EC=3.6.4.13;
DE   AltName: Full=DEAH box protein 35;
GN   Name=DHX35;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May be involved in pre-mRNA splicing. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Identified in the spliceosome C complex. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CR858717; CAH90926.1; -; mRNA.
DR   RefSeq; NP_001125530.1; NM_001132058.1.
DR   AlphaFoldDB; Q5RBD4; -.
DR   SMR; Q5RBD4; -.
DR   STRING; 9601.ENSPPYP00000012292; -.
DR   Ensembl; ENSPPYT00000012773; ENSPPYP00000012292; ENSPPYG00000011007.
DR   GeneID; 100172442; -.
DR   KEGG; pon:100172442; -.
DR   CTD; 60625; -.
DR   eggNOG; KOG0922; Eukaryota.
DR   GeneTree; ENSGT00940000156142; -.
DR   InParanoid; Q5RBD4; -.
DR   OrthoDB; 354219at2759; -.
DR   Proteomes; UP000001595; Chromosome 20.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IEA:Ensembl.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001701; P:in utero embryonic development; IEA:Ensembl.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR   InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF07717; OB_NTP_bind; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00847; HA2; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; mRNA processing; mRNA splicing;
KW   Nucleotide-binding; Reference proteome; Spliceosome.
FT   CHAIN           1..720
FT                   /note="Probable ATP-dependent RNA helicase DHX35"
FT                   /id="PRO_0000055169"
FT   DOMAIN          64..229
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          261..438
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           176..179
FT                   /note="DEAH box"
FT   BINDING         77..84
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   720 AA;  80616 MW;  FADA86F0D52EFEA6 CRC64;
     MAAPVGLVKF WRPGTEGPGV SISEERQSLA ENSGTTVVYN PYAALSIEQQ RQKLPVFKLR
     NHILYLIENY QTVVIVGETG CGKSTQIPQY LAEAGWTAEG RVVGVTQPRR VAAVTVAGRV
     AEERGAVLGH EVGYCIRFDD CTDQLATRIK FLTDGMLVRE MMVDPLLTKY SVIMLDEAHE
     RTLYTDIAIG LLKKIQKKRG DLRLIVASAT LDADKFRDFF NQNETSDPAR DTCVILTVGG
     RTFPVDIFYL QSPVPDYIKS TVETVVKIHQ TEGDGDILAF LTGQEEVETV VSMLIEQARA
     LARTGMKRHL RVLPMYAGLP SFEQMKVFER VSRSVRKVIV ATNVAETSIT ISGIVYVIDC
     GFVKLRAYNP RTAIECLVVV PVSQASANQR AGRGGRSRSG KCYRLYTEEA FDKLPQSTVP
     EMQRSNLAPV ILQLKALGID NVLRFHFMSP PPAQSMVQAL ELLYALGGLD KDCRLTEPLG
     MRIAEFPLNP MFAKMLLESG NFGCSQEILS IAAMMQIQNI FVVPPNQKSQ AIRVHRKFAV
     EEGDHLTMLN VYEAFIKHNK NSQWCQEHFL NYKGLVRAAT VREQLKKLLV KFQVPKKSSE
     GDPDPVLRCI VSGFFANAAR FHSTGAYRTI RDDHELHIHP ASVLYAEKPP RWVIYNEVIQ
     TSKYYMRDVT AIESAWLLEL APHFYQQGTQ ACTLHVGRGR VFISILEHGY LACRDACTCL