ID   DHYS1_METAC             Reviewed;         317 AA.
AC   Q8TS38;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Probable deoxyhypusine synthase 1;
DE            Short=DHS 1;
DE            EC=2.5.1.46;
GN   Name=dys1; OrderedLocusNames=MA_0968;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the NAD-dependent oxidative cleavage of spermidine
CC       and the subsequent transfer of the butylamine moiety of spermidine to
CC       the epsilon-amino group of a specific lysine residue of the eIF-5A
CC       precursor protein to form the intermediate deoxyhypusine residue.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-
CC         deoxyhypusine + propane-1,3-diamine; Xref=Rhea:RHEA:33299, Rhea:RHEA-
CC         COMP:10143, Rhea:RHEA-COMP:10144, ChEBI:CHEBI:29969,
CC         ChEBI:CHEBI:57484, ChEBI:CHEBI:57834, ChEBI:CHEBI:82657; EC=2.5.1.46;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- PATHWAY: Protein modification; eIF5A hypusination.
CC   -!- SIMILARITY: Belongs to the deoxyhypusine synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AE010299; AAM04400.1; -; Genomic_DNA.
DR   RefSeq; WP_011021005.1; NC_003552.1.
DR   AlphaFoldDB; Q8TS38; -.
DR   SMR; Q8TS38; -.
DR   STRING; 188937.MA_0968; -.
DR   PRIDE; Q8TS38; -.
DR   EnsemblBacteria; AAM04400; AAM04400; MA_0968.
DR   GeneID; 1472858; -.
DR   KEGG; mac:MA_0968; -.
DR   HOGENOM; CLU_039781_1_0_2; -.
DR   InParanoid; Q8TS38; -.
DR   OMA; EARSWGK; -.
DR   OrthoDB; 35308at2157; -.
DR   PhylomeDB; Q8TS38; -.
DR   UniPathway; UPA00354; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0034038; F:deoxyhypusine synthase activity; IBA:GO_Central.
DR   GO; GO:0008612; P:peptidyl-lysine modification to peptidyl-hypusine; IBA:GO_Central.
DR   Gene3D; 3.40.910.10; -; 1.
DR   HAMAP; MF_00153; DHS; 1.
DR   InterPro; IPR022899; Deoxyhypus_synthase_arc.
DR   InterPro; IPR002773; Deoxyhypusine_synthase.
DR   InterPro; IPR036982; Deoxyhypusine_synthase_sf.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   PANTHER; PTHR11703; PTHR11703; 1.
DR   Pfam; PF01916; DS; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   TIGRFAMs; TIGR00321; dhys; 1.
PE   3: Inferred from homology;
KW   Hypusine biosynthesis; NAD; Reference proteome; Transferase.
FT   CHAIN           1..317
FT                   /note="Probable deoxyhypusine synthase 1"
FT                   /id="PRO_0000134493"
FT   ACT_SITE        285
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   317 AA;  34596 MW;  90767A610F775474 CRC64;
     MDFEASARDL TTPVKGAKIV PNMSVDELVR EYAGCAFGAG RLAEAVDIYY EMLASEKTTK
     FFGLAGAMTP AGMRGIIADL IRDGHIDVLV TTGANMVHDT VEALGLHHYK GSDCANDIQL
     RHECIDRIYD VYLPDQHFTD LEEFLQSVYA GLPQEKLSIR QVLTEIGKNL DDDSSILKTA
     AEMGVPVYCP ALQDSVIGLQ AWLYKEGNLL HVDAFADMHE FMEICYEAES AGAMLLGGGV
     PKNYILQSML VTPKSFDYAI QLTMDRPETG GLSGATLDEA QSWGKVGEDA KSVTVYADAT
     ITLPLIVSAV RTRLSKR