DHYS2_ARCFU
ID DHYS2_ARCFU Reviewed; 298 AA.
AC O27984;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Probable deoxyhypusine synthase 2;
DE Short=DHS 2;
DE EC=2.5.1.46;
GN Name=dys2; OrderedLocusNames=AF_2300;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
CC -!- FUNCTION: Catalyzes the NAD-dependent oxidative cleavage of spermidine
CC and the subsequent transfer of the butylamine moiety of spermidine to
CC the epsilon-amino group of a specific lysine residue of the eIF-5A
CC precursor protein to form the intermediate deoxyhypusine residue.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-
CC deoxyhypusine + propane-1,3-diamine; Xref=Rhea:RHEA:33299, Rhea:RHEA-
CC COMP:10143, Rhea:RHEA-COMP:10144, ChEBI:CHEBI:29969,
CC ChEBI:CHEBI:57484, ChEBI:CHEBI:57834, ChEBI:CHEBI:82657; EC=2.5.1.46;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC -!- PATHWAY: Protein modification; eIF5A hypusination.
CC -!- SIMILARITY: Belongs to the deoxyhypusine synthase family.
CC {ECO:0000305}.
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DR EMBL; AE000782; AAB88953.1; -; Genomic_DNA.
DR PIR; D69537; D69537.
DR RefSeq; WP_010879789.1; NC_000917.1.
DR AlphaFoldDB; O27984; -.
DR SMR; O27984; -.
DR STRING; 224325.AF_2300; -.
DR EnsemblBacteria; AAB88953; AAB88953; AF_2300.
DR GeneID; 1485532; -.
DR KEGG; afu:AF_2300; -.
DR eggNOG; arCOG04142; Archaea.
DR HOGENOM; CLU_039781_1_0_2; -.
DR OMA; YTSSPGD; -.
DR OrthoDB; 35308at2157; -.
DR PhylomeDB; O27984; -.
DR UniPathway; UPA00354; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0034038; F:deoxyhypusine synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008612; P:peptidyl-lysine modification to peptidyl-hypusine; IEA:UniProtKB-KW.
DR Gene3D; 3.40.910.10; -; 1.
DR HAMAP; MF_00153; DHS; 1.
DR InterPro; IPR022899; Deoxyhypus_synthase_arc.
DR InterPro; IPR002773; Deoxyhypusine_synthase.
DR InterPro; IPR036982; Deoxyhypusine_synthase_sf.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR PANTHER; PTHR11703; PTHR11703; 1.
DR Pfam; PF01916; DS; 1.
DR SUPFAM; SSF52467; SSF52467; 1.
DR TIGRFAMs; TIGR00321; dhys; 1.
PE 3: Inferred from homology;
KW Hypusine biosynthesis; NAD; Reference proteome; Transferase.
FT CHAIN 1..298
FT /note="Probable deoxyhypusine synthase 2"
FT /id="PRO_0000134491"
FT ACT_SITE 259
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 298 AA; 33015 MW; E9E7ADE0C751E33B CRC64;
MARTGFQGRK LGEAFEIWTE MLREENITIL MGLSGAMVPA GMRKIIAWLI RNRYIDVLVS
TGANLFHDIH EAMGFRHFMG SEHVNDCKLF EEGIDRIHDV FAYEKEFNVI DYTLAEIISE
MSGVMSSREF LEEIAGRLNV KDRNSIVIAA YESKVPIFSP AIADSSIGIA AALAKREVVI
DTIRDVEELT EIVVNSEKTG VIYVGGGVPK NFIQQTEVVA RLKGYDVRGH EYAIQITTDV
PQFGGLSGCT FEEGVSWGKI SGKAKKVQVN CDATIALPVL AHGLIGIRRQ KYPIFNGL