ID   DHYS2_METAC             Reviewed;         345 AA.
AC   Q8TLM3;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Probable deoxyhypusine synthase 2;
DE            Short=DHS 2;
DE            EC=2.5.1.46;
GN   Name=dys2; OrderedLocusNames=MA_3013;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the NAD-dependent oxidative cleavage of spermidine
CC       and the subsequent transfer of the butylamine moiety of spermidine to
CC       the epsilon-amino group of a specific lysine residue of the eIF-5A
CC       precursor protein to form the intermediate deoxyhypusine residue.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-
CC         deoxyhypusine + propane-1,3-diamine; Xref=Rhea:RHEA:33299, Rhea:RHEA-
CC         COMP:10143, Rhea:RHEA-COMP:10144, ChEBI:CHEBI:29969,
CC         ChEBI:CHEBI:57484, ChEBI:CHEBI:57834, ChEBI:CHEBI:82657; EC=2.5.1.46;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- PATHWAY: Protein modification; eIF5A hypusination.
CC   -!- SIMILARITY: Belongs to the deoxyhypusine synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AE010299; AAM06386.1; -; Genomic_DNA.
DR   RefSeq; WP_011022952.1; NC_003552.1.
DR   AlphaFoldDB; Q8TLM3; -.
DR   SMR; Q8TLM3; -.
DR   STRING; 188937.MA_3013; -.
DR   EnsemblBacteria; AAM06386; AAM06386; MA_3013.
DR   GeneID; 1474907; -.
DR   KEGG; mac:MA_3013; -.
DR   HOGENOM; CLU_039781_1_0_2; -.
DR   InParanoid; Q8TLM3; -.
DR   OMA; YTSSPGD; -.
DR   OrthoDB; 35308at2157; -.
DR   PhylomeDB; Q8TLM3; -.
DR   UniPathway; UPA00354; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0034038; F:deoxyhypusine synthase activity; IBA:GO_Central.
DR   GO; GO:0008612; P:peptidyl-lysine modification to peptidyl-hypusine; IBA:GO_Central.
DR   Gene3D; 3.40.910.10; -; 1.
DR   HAMAP; MF_00153; DHS; 1.
DR   InterPro; IPR022899; Deoxyhypus_synthase_arc.
DR   InterPro; IPR002773; Deoxyhypusine_synthase.
DR   InterPro; IPR036982; Deoxyhypusine_synthase_sf.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   PANTHER; PTHR11703; PTHR11703; 1.
DR   Pfam; PF01916; DS; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   TIGRFAMs; TIGR00321; dhys; 1.
PE   3: Inferred from homology;
KW   Hypusine biosynthesis; NAD; Reference proteome; Transferase.
FT   CHAIN           1..345
FT                   /note="Probable deoxyhypusine synthase 2"
FT                   /id="PRO_0000134494"
FT   ACT_SITE        292
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   345 AA;  38274 MW;  4910D9B768E17A45 CRC64;
     MHHNVFTNNP TVPIDVKNRS VSELMDGMLK TGFQGRKLAE SVQAWNNMLK EKNTTVFMGL
     SGAMVPAGMR RIISHMIRER MIDCLVSTGA NLFHDCHEAL GRKHYVGSHL ANDEKLFEQG
     VDRIYDIFAV EEEFRTADNL IADFAQEIGE ITCSSREFMY LLGKELGRRG AAEDSIVVSA
     YRHNVPIFVP ALSDSSIGIG LTIARRRGLK LEIDQIKDVD EITQIVEKSE RTGVVYVGGG
     VPKNFIQQTE VIASILGMDI GGHDYAIQYT SDSPHWGGLS GCTFDEAVSW GKIAPQAKKV
     QVFVDATIAL PIVAHALHEK THDLKRIAPV FFWDGPEGLA IDYKE