ID   DHYS_METTE              Reviewed;         317 AA.
AC   Q977X6;
DT   31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2002, sequence version 2.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Probable deoxyhypusine synthase;
DE            Short=DHS;
DE            EC=2.5.1.46;
GN   Name=dys; Synonyms=dhs;
OS   Methanosarcina thermophila.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2210;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Apolinario-Smith E.E., Sowers K.R.;
RT   "A putative pyrF gene cloned from Methanosarcina thermophila.";
RL   Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the NAD-dependent oxidative cleavage of spermidine
CC       and the subsequent transfer of the butylamine moiety of spermidine to
CC       the epsilon-amino group of a specific lysine residue of the eIF-5A
CC       precursor protein to form the intermediate deoxyhypusine residue.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-
CC         deoxyhypusine + propane-1,3-diamine; Xref=Rhea:RHEA:33299, Rhea:RHEA-
CC         COMP:10143, Rhea:RHEA-COMP:10144, ChEBI:CHEBI:29969,
CC         ChEBI:CHEBI:57484, ChEBI:CHEBI:57834, ChEBI:CHEBI:82657; EC=2.5.1.46;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- PATHWAY: Protein modification; eIF5A hypusination.
CC   -!- SIMILARITY: Belongs to the deoxyhypusine synthase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK49974.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AF358260; AAK49974.1; ALT_FRAME; Genomic_DNA.
DR   AlphaFoldDB; Q977X6; -.
DR   SMR; Q977X6; -.
DR   UniPathway; UPA00354; -.
DR   GO; GO:0034038; F:deoxyhypusine synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008612; P:peptidyl-lysine modification to peptidyl-hypusine; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.910.10; -; 1.
DR   HAMAP; MF_00153; DHS; 1.
DR   InterPro; IPR022899; Deoxyhypus_synthase_arc.
DR   InterPro; IPR002773; Deoxyhypusine_synthase.
DR   InterPro; IPR036982; Deoxyhypusine_synthase_sf.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   PANTHER; PTHR11703; PTHR11703; 1.
DR   Pfam; PF01916; DS; 1.
DR   SUPFAM; SSF52467; SSF52467; 1.
DR   TIGRFAMs; TIGR00321; dhys; 1.
PE   3: Inferred from homology;
KW   Hypusine biosynthesis; NAD; Transferase.
FT   CHAIN           1..317
FT                   /note="Probable deoxyhypusine synthase"
FT                   /id="PRO_0000134499"
FT   ACT_SITE        285
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   317 AA;  34675 MW;  A78E8CB4B51EA4F4 CRC64;
     MDFEASAKIL TTPVKGAKIV SNMTVDELVR EYAGCAFGAG RLAKAVDIYY EMLASEKTTK
     FFGLAGAMTP AGMRNIIADL IRDGYIDVLV TTGANMVHDT VEALGLHHYK GSDCTNDIQL
     RHECIDRIYD VYLPDQHFTD LEEFLQGVYA GLPQEKLSIR QVLTEIGKNL DDDSSILKTA
     AEMGVPVYCP ALQDSVIGLQ AWLYKEGNPL HVDAFADMHE FMEICFETES AGAMLLGGGV
     PKNYILQSML VTPRSFDYAI QLTMDHPETG GLSGATLDEA QSWGKVGENA KSVTVYADST
     ITLPLIVSAV RTRLSKR