DI191_ARATH
ID DI191_ARATH Reviewed; 206 AA.
AC Q39083; F4I523; Q56ZC1; Q94AR0; Q9C7K3;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Protein DEHYDRATION-INDUCED 19;
DE Short=AtDi19-1;
GN Name=DI19-1; Synonyms=DI19; OrderedLocusNames=At1g56280; ORFNames=F14G9.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION BY DROUGHT.
RC STRAIN=cv. Columbia; TISSUE=Root;
RX PubMed=7823904; DOI=10.1007/bf00290128;
RA Gosti F., Bertauche N., Vartanian N., Giraudat J.;
RT "Abscisic acid-dependent and -independent regulation of gene expression by
RT progressive drought in Arabidopsis thaliana.";
RL Mol. Gen. Genet. 246:10-18(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 121-206.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INTERACTION WITH LKP2, AND SUBCELLULAR LOCATION.
RX PubMed=15937324; DOI=10.1093/pcp/pci144;
RA Fukamatsu Y., Mitsui S., Yasuhara M., Tokioka Y., Ihara N., Fujita S.,
RA Kiyosue T.;
RT "Identification of LOV KELCH PROTEIN2 (LKP2)-interacting factors that can
RT recruit LKP2 to nuclear bodies.";
RL Plant Cell Physiol. 46:1340-1349(2005).
RN [8]
RP INTERACTION WITH CPK4; CPK11 AND CPK12, SUBCELLULAR LOCATION, AND
RP PHOSPHORYLATION AT THR-105 AND SER-107.
RX PubMed=16438971; DOI=10.1016/j.febslet.2006.01.013;
RA Rodriguez Milla M.A., Uno Y., Chang I.-F., Townsend J., Maher E.A.,
RA Quilici D., Cushman J.C.;
RT "A novel yeast two-hybrid approach to identify CDPK substrates:
RT characterization of the interaction between AtCPK11 and AtDi19, a nuclear
RT zinc finger protein.";
RL FEBS Lett. 580:904-911(2006).
RN [9]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION, INDUCTION BY
RP DROUGHT, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16786289; DOI=10.1007/s11103-005-5798-7;
RA Rodriguez Milla M.A., Townsend J., Chang I.-F., Cushman J.C.;
RT "The Arabidopsis AtDi19 gene family encodes a novel type of Cys2/His2 zinc-
RT finger protein implicated in ABA-independent dehydration, high-salinity
RT stress and light signaling pathways.";
RL Plant Mol. Biol. 61:13-30(2006).
CC -!- SUBUNIT: Interacts with ADO2/LKP2, CPK11 and CPK4. Weak interaction
CC with CPK12 and no interactions with CPK1, CPK5 or CPK26.
CC {ECO:0000269|PubMed:15937324, ECO:0000269|PubMed:16438971}.
CC -!- INTERACTION:
CC Q39083; Q39016: CPK11; NbExp=25; IntAct=EBI-979339, EBI-979321;
CC Q39083; Q38869: CPK4; NbExp=5; IntAct=EBI-979339, EBI-979475;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15937324,
CC ECO:0000269|PubMed:16438971, ECO:0000269|PubMed:16786289}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q39083-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, roots, leaves, stems,
CC flowers and siliques. {ECO:0000269|PubMed:16786289}.
CC -!- INDUCTION: By drought stress, but not by abscisic acid.
CC {ECO:0000269|PubMed:16786289, ECO:0000269|PubMed:7823904}.
CC -!- DOMAIN: The NLS/NES region (98-121) is necessary and sufficient for
CC interaction with CPK11.
CC -!- PTM: Phosphorylated within the NLS/NES region.
CC {ECO:0000269|PubMed:16438971, ECO:0000269|PubMed:16786289}.
CC -!- SIMILARITY: Belongs to the Di19 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50925.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG50925.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AEE33372.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X78584; CAA55321.1; -; mRNA.
DR EMBL; AC069159; AAG50925.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33372.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AY045868; AAK76542.1; -; mRNA.
DR EMBL; BT020564; AAW70410.1; -; mRNA.
DR EMBL; AK221045; BAD94799.1; -; mRNA.
DR PIR; E96604; E96604.
DR PIR; S51478; S51478.
DR RefSeq; NP_564715.4; NM_104507.4.
DR AlphaFoldDB; Q39083; -.
DR BioGRID; 27306; 7.
DR DIP; DIP-37941N; -.
DR IntAct; Q39083; 4.
DR MINT; Q39083; -.
DR STRING; 3702.AT1G56280.1; -.
DR iPTMnet; Q39083; -.
DR PRIDE; Q39083; -.
DR GeneID; 842081; -.
DR KEGG; ath:AT1G56280; -.
DR Araport; AT1G56280; -.
DR eggNOG; ENOG502QW9I; Eukaryota.
DR InParanoid; Q39083; -.
DR PhylomeDB; Q39083; -.
DR PRO; PR:Q39083; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q39083; baseline and differential.
DR Genevisible; Q39083; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR InterPro; IPR033347; DI19.
DR InterPro; IPR027935; Di19_C.
DR InterPro; IPR008598; Di19_Zn-bd.
DR PANTHER; PTHR31875; PTHR31875; 1.
DR Pfam; PF14571; Di19_C; 1.
DR Pfam; PF05605; zf-Di19; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..206
FT /note="Protein DEHYDRATION-INDUCED 19"
FT /id="PRO_0000304413"
FT REGION 142..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 142..161
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 105
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:16438971"
FT MOD_RES 107
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:16438971"
FT CONFLICT 112
FT /note="E -> G (in Ref. 1; CAA55321)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 206 AA; 23275 MW; F1E96902AB60B74B CRC64;
MDADSKRFLA TLRSRSEMLM GFEEIDGDDD FQEEFACPFC AESYDIIGLC CHIDDEHTLE
SKNAVCPVCS LKVGVDIVAH ITLHHGSLFK LQRKRKSRKS GTNSTLSLLR KELREGDLQR
LLGFTSRNGS VASSVTPDPL LSSFISPTRS QSSPAPRQTK NVSEDKQIER KRQVFISPVS
LKDREERRHK SEFVQRLLSS AIFDEV
