DIA1_DICDI
ID DIA1_DICDI Reviewed; 455 AA.
AC Q54N81; Q9GV74;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=Differentiation-associated protein 1;
DE Short=DIA-1;
DE Flags: Precursor;
GN Name=dia1; ORFNames=DDB_G0285431;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX2;
RX PubMed=10887082; DOI=10.1242/dev.127.15.3263;
RA Hirose S., Inazu Y., Chae S.-C., Maeda Y.;
RT "Suppression of the growth/differentiation transition in Dictyostelium
RT development by transient expression of a novel gene, dia1.";
RL Development 127:3263-3270(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=15507488; DOI=10.1242/jcs.01499;
RA Morita T., Amagai A., Maeda Y.;
RT "Translocation of the Dictyostelium TRAP1 homologue to mitochondria induces
RT a novel prestarvation response.";
RL J. Cell Sci. 117:5759-5770(2004).
CC -!- FUNCTION: Plays a role in differentiation.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Specifically expressed in cells differentiation
CC from the PS point, a specific point from which cells enter the
CC differentiation phase in response to starvation. Not present in the
CC exponentially growing cells, starts to accumulate within 2 hours
CC following removal of growth medium, reaching a peak at 4 hours then
CC disappearing. {ECO:0000269|PubMed:10887082,
CC ECO:0000269|PubMed:15507488}.
CC -!- MISCELLANEOUS: Overexpression rather impaired the progression of
CC differentiation. Antisense RNA-mediated dia1 inactivation was found to
CC enhance the initial step of cell differentiation.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB13513.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB007026; BAB13513.1; ALT_FRAME; mRNA.
DR EMBL; AAFI02000079; EAL64553.1; -; Genomic_DNA.
DR RefSeq; XP_638062.1; XM_632970.1.
DR AlphaFoldDB; Q54N81; -.
DR STRING; 44689.DDB0191142; -.
DR PaxDb; Q54N81; -.
DR EnsemblProtists; EAL64553; EAL64553; DDB_G0285431.
DR GeneID; 8625108; -.
DR KEGG; ddi:DDB_G0285431; -.
DR dictyBase; DDB_G0285431; dia1.
DR eggNOG; ENOG502RHXC; Eukaryota.
DR HOGENOM; CLU_601917_0_0_1; -.
DR InParanoid; Q54N81; -.
DR OMA; IACANSH; -.
DR PhylomeDB; Q54N81; -.
DR PRO; PR:Q54N81; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009267; P:cellular response to starvation; IEP:dictyBase.
PE 2: Evidence at transcript level;
KW Cell membrane; Differentiation; Glycoprotein; GPI-anchor; Lipoprotein;
KW Membrane; Reference proteome; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..433
FT /note="Differentiation-associated protein 1"
FT /id="PRO_0000361776"
FT PROPEP 434..455
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000361777"
FT REGION 349..434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 433
FT /note="GPI-like-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CONFLICT 26
FT /note="F -> S (in Ref. 1; BAB13513)"
FT /evidence="ECO:0000305"
FT CONFLICT 85..101
FT /note="IGTQYPSSQCKQNSNCL -> MGTHILRHNVTELKLF (in Ref. 1;
FT BAB13513)"
FT /evidence="ECO:0000305"
FT CONFLICT 342..343
FT /note="SD -> RY (in Ref. 1; BAB13513)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 455 AA; 48158 MW; 08440420DCAC75F0 CRC64;
MKFKLFLLVF FVFLLPYLSQ SCENTFSCPT LNQTCTGQCS AGLVCNQTLS SCQEYEKCDS
SLPLAEDTCS QDGYICVSNV CLPFIGTQYP SSQCKQNSNC LMSLCAGNIC MVMPGSQCQT
NSQCSPDQFC STTFIGTTST SPSTMTLPMT MTLPMTMTLP ITMTMGSSSV CTNRLQLNSQ
CSTSDSCQTG LACINSVCSP IFSGAENATC FPGAIEPTKA ACDVGLSCLN GANGYSCKSY
VENQSCDPSD EYPVCNSDYQ SCKCNSKGKG SCQSYYKLTQ ECKDSSNKLV LCAKSKNSIP
SYKDYVTQIN CQSQLCNYSR DCIDPKAKVS TCFNDLFLMC PSDYQEPEIG SSSSSSSSSS
SSGSSSNSTS SSTSSTSSTS SESSESSNGS NSNSVSSESS SPSSSSVESS SNSKSNHTSS
ESSSSDDDLG NPSSSSILSV SKLIILLISI ILYCF
