ID   DIA2_ASHGO              Reviewed;         685 AA.
AC   Q75A03;
DT   18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Protein DIA2;
GN   Name=DIA2; OrderedLocusNames=ADR230W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 69-70 AND 80.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: F-box protein component of a SCF ubiquitin ligase complex
CC       involved in ubiquitin-dependent protein degradation. The SCF-DIA2
CC       complex is specifically involved in the pheromone induced degradation
CC       of phosphorylated TEC1. Involved in DNA replication, genome stability,
CC       and the control of cell cycle, probably through its association to
CC       replication origins to facilitate the ubiquitination of another origin-
CC       binding protein (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms a SCF ubiquitin ligase complex which binds to DNA
CC       replication origins. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DIA2 family. {ECO:0000305}.
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DR   EMBL; AE016817; AAS52150.2; -; Genomic_DNA.
DR   RefSeq; NP_984326.2; NM_209679.2.
DR   AlphaFoldDB; Q75A03; -.
DR   SMR; Q75A03; -.
DR   STRING; 33169.AAS52150; -.
DR   EnsemblFungi; AAS52150; AAS52150; AGOS_ADR230W.
DR   GeneID; 4620488; -.
DR   KEGG; ago:AGOS_ADR230W; -.
DR   eggNOG; ENOG502QRSD; Eukaryota.
DR   HOGENOM; CLU_023422_0_0_1; -.
DR   InParanoid; Q75A03; -.
DR   OMA; ISCKGYL; -.
DR   Proteomes; UP000000591; Chromosome IV.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   Gene3D; 1.25.40.10; -; 1.
DR   Gene3D; 3.80.10.10; -; 1.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   InterPro; IPR001810; F-box_dom.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   Pfam; PF12937; F-box-like; 1.
DR   SMART; SM00028; TPR; 2.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   SUPFAM; SSF81383; SSF81383; 1.
DR   PROSITE; PS50181; FBOX; 1.
DR   PROSITE; PS50005; TPR; 2.
DR   PROSITE; PS50293; TPR_REGION; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Leucine-rich repeat; Nucleus; Reference proteome; Repeat;
KW   TPR repeat; Ubl conjugation pathway.
FT   CHAIN           1..685
FT                   /note="Protein DIA2"
FT                   /id="PRO_0000233002"
FT   REPEAT          6..39
FT                   /note="TPR 1"
FT   REPEAT          70..103
FT                   /note="TPR 2"
FT   REPEAT          104..137
FT                   /note="TPR 3"
FT   DOMAIN          207..254
FT                   /note="F-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT   REPEAT          300..323
FT                   /note="LRR 1"
FT   REPEAT          353..377
FT                   /note="LRR 2"
FT   REPEAT          454..480
FT                   /note="LRR 3"
FT   REPEAT          499..517
FT                   /note="LRR 4"
FT   REPEAT          518..542
FT                   /note="LRR 5"
FT   REPEAT          562..585
FT                   /note="LRR 6"
FT   REPEAT          598..622
FT                   /note="LRR 7"
SQ   SEQUENCE   685 AA;  79100 MW;  8CB95476C40EFA3A CRC64;
     MSEDIIDRSL ELGIQCFQGE DYKGAAELFS KSLQLARSYT DRSLEGIREK VGLPKRCLHD
     PSRVYHPRYL VLLDNRAATW EKLNKLDRAL ADAAYMITVD AYNLKGYIRR GKVLQKLGRY
     EEALQVYENG LKQAGEAEKT HAIHAPQKFL DIVYRQRSTI KELLQSRARS SRSLTQQTVA
     KEPKLKRPAT IDSLMPGKKR SSSKAKIDYI ATLPVEIIER IMANMDTRSI IRCYSVCKLW
     KYRLERLPHL YQEFRLSCCY KNMLGYVNFV GALASRTAEY SCRSIQCVSG NVQEEEKSII
     LLLSRLMIGT RQLALMAKKC KAERIIQHIC ENKKLRNGVQ RLSITAPVHF GHKLNLHELY
     TRTTSMTHLE LVLNFHTPSE HVGGHLFPWQ DHAVAETNLE SFTLMARNYS VHHVNVIEQF
     EYSSVLFKRL KKLCITGIDF RLGDRNNLKW ISDMPNLQEL WLERNTGIEF HELITQIVQV
     GAPKTLRHLT FREPPNRHFD RMDQSQLGLG EEALREVFQS LESLDLMNTR FDPQLLLLLL
     QPACENRIAR LNIGNCPRLS FARDLEILTL IFQQLPALTD LLLPNVMEYT RQGMEVLRKN
     IKGMKLKRLD LSFIPSLKGY ELLDLLKELK GINPLGLETL TINGCTAVAP QTVDYITRNG
     YAQKVMCAYE RTQWEHLGIN SFWYR