ID   DIA2_DICDI              Reviewed;         151 AA.
AC   O96042; Q54EI3;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Differentiation-associated protein 2;
DE            Short=DIA-2;
DE   Flags: Precursor;
GN   Name=dia2; ORFNames=DDB_G0291253;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=9813183; DOI=10.1006/bbrc.1998.9461;
RA   Chae S.-C., Inazu Y., Amagai A., Maeda Y.;
RT   "Underexpression of a novel gene, dia2, impairs the transition of
RT   Dictyostelium cells from growth to differentiation.";
RL   Biochem. Biophys. Res. Commun. 252:278-283(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, SYNTHESIS OF 77-90, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=17825085; DOI=10.1111/j.1432-0436.2007.00217.x;
RA   Hirata K., Amagai A., Chae S.C., Hirose S., Maeda Y.;
RT   "Involvements of a novel protein, DIA2, in cAMP signaling and spore
RT   differentiation during Dictyostelium development.";
RL   Differentiation 76:310-322(2008).
CC   -!- FUNCTION: Has an essential role in the initiation of differentiation.
CC       Also required for cAMP signaling. {ECO:0000269|PubMed:17825085,
CC       ECO:0000269|PubMed:9813183}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC       {ECO:0000269|PubMed:17825085}. Vacuole {ECO:0000269|PubMed:17825085}.
CC       Note=During late development it changes it's location from endoplasmic
CC       reticulum to pre-spore-specific vacuoles.
CC   -!- DEVELOPMENTAL STAGE: Accumulates in differentiating cells starved just
CC       before the PS point, while there is no detectable expression in
CC       vegetatively growing cells. {ECO:0000269|PubMed:17825085,
CC       ECO:0000269|PubMed:9813183}.
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DR   EMBL; AB007027; BAA34858.1; -; mRNA.
DR   EMBL; AAFI02000177; EAL61610.1; -; Genomic_DNA.
DR   RefSeq; XP_635257.1; XM_630165.1.
DR   AlphaFoldDB; O96042; -.
DR   STRING; 44689.DDB0185193; -.
DR   PaxDb; O96042; -.
DR   EnsemblProtists; EAL61610; EAL61610; DDB_G0291253.
DR   GeneID; 8628204; -.
DR   KEGG; ddi:DDB_G0291253; -.
DR   dictyBase; DDB_G0291253; dia2.
DR   HOGENOM; CLU_1734893_0_0_1; -.
DR   InParanoid; O96042; -.
DR   PhylomeDB; O96042; -.
DR   PRO; PR:O96042; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:dictyBase.
DR   GO; GO:0030141; C:secretory granule; IDA:dictyBase.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0140582; P:adenylate cyclase-activating G protein-coupled cAMP receptor signaling pathway; IMP:dictyBase.
DR   GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0031154; P:culmination involved in sorocarp development; IMP:dictyBase.
PE   2: Evidence at transcript level;
KW   Differentiation; Endoplasmic reticulum; Reference proteome; Signal;
KW   Vacuole.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..151
FT                   /note="Differentiation-associated protein 2"
FT                   /id="PRO_0000361778"
SQ   SEQUENCE   151 AA;  16945 MW;  28EF33AADD75CC1B CRC64;
     MKQIIRLITT LLLLSLIGIT CAAVAKLHET DKKFKGVELP EKFLDDFNLE VGKLQYANLF
     RSKKDDITHS AGEIRIDQQG SFSRGEKKKK FKVYNLQAQT NGKSSKTVAH VEVFDTIDAK
     TKAEQNEVLA LAKEAFTKSK DSGKLYQVIP N