ID   DIAC_BOVIN              Reviewed;         175 AA.
AC   Q01458;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Di-N-acetylchitobiase;
DE            EC=3.2.1.-;
DE   Flags: Precursor; Fragment;
GN   Name=CTBS; Synonyms=CTB;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1527079; DOI=10.1016/s0021-9258(18)41818-2;
RA   Fisher K.J., Aronson N.N. Jr.;
RT   "Cloning and expression of the cDNA sequence encoding the lysosomal
RT   glycosidase di-N-acetylchitobiase.";
RL   J. Biol. Chem. 267:19607-19616(1992).
CC   -!- FUNCTION: Involved in the degradation of asparagine-linked
CC       glycoproteins. Hydrolyze of N-acetyl-beta-D-glucosamine (1-4)N-
CC       acetylglucosamine chitobiose core from the reducing end of the bond, it
CC       requires prior cleavage by glycosylasparaginase.
CC   -!- SUBCELLULAR LOCATION: Lysosome.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. {ECO:0000305}.
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DR   EMBL; M95771; AAA30444.1; -; Genomic_DNA.
DR   EMBL; M95770; AAA30444.1; JOINED; Genomic_DNA.
DR   EMBL; M95769; AAA30444.1; JOINED; Genomic_DNA.
DR   PIR; B44102; B44102.
DR   AlphaFoldDB; Q01458; -.
DR   SMR; Q01458; -.
DR   STRING; 9913.ENSBTAP00000007603; -.
DR   CAZy; GH18; Glycoside Hydrolase Family 18.
DR   PaxDb; Q01458; -.
DR   PRIDE; Q01458; -.
DR   eggNOG; KOG2806; Eukaryota.
DR   InParanoid; Q01458; -.
DR   OrthoDB; 826687at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004568; F:chitinase activity; IBA:GO_Central.
DR   GO; GO:0006032; P:chitin catabolic process; IBA:GO_Central.
DR   GO; GO:0009313; P:oligosaccharide catabolic process; IBA:GO_Central.
DR   InterPro; IPR001223; Glyco_hydro18_cat.
DR   InterPro; IPR001579; Glyco_hydro_18_chit_AS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS01095; GH18_1; 1.
DR   PROSITE; PS51910; GH18_2; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Glycosidase; Hydrolase; Lysosome; Reference proteome; Signal.
FT   SIGNAL          1..38
FT                   /evidence="ECO:0000250"
FT   CHAIN           39..>175
FT                   /note="Di-N-acetylchitobiase"
FT                   /id="PRO_0000011960"
FT   DOMAIN          39..>175
FT                   /note="GH18"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01258"
FT   ACT_SITE        143
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01258"
FT   CARBOHYD        115
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   NON_TER         175
SQ   SEQUENCE   175 AA;  19761 MW;  BB70F981BB5D5064 CRC64;
     MARLQLAGSR RLVPLPRRAP RLAPLLLPLL LALPDGARAD CPCKVPALCR PMTHRPDFEV
     FVFNVGHKTW KYYDWSQITT VVLFLKYDPE LMCHAHAKGA RVVLKGDVPV KDIINATFRA
     SWIAQQVKLA KTQYMDGINL DIEQDVAHSS PEYYALTALV KETTDSFHHE IKGSQ