DID2_SCHPO
ID DID2_SCHPO Reviewed; 200 AA.
AC O60074;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Vacuolar protein-sorting-associated protein 46;
DE AltName: Full=Charged multivesicular body protein 1;
DE AltName: Full=Doa4-independent degradation protein 2;
GN Name=did2;
GN Synonyms=chm1 {ECO:0000250|UniProtKB:P69771},
GN vps46 {ECO:0000250|UniProtKB:P69771}; ORFNames=SPBC13G1.12;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
CC -!- FUNCTION: Class E VPS protein implicated in concentration and sorting
CC of cargo proteins of the multivesicular body (MVB) for incorporation
CC into intralumenal vesicles. The lumenal sequestrated membrane proteins
CC will be targeted into the vacuole after fusion of the endosome with the
CC vacuole (By similarity). {ECO:0000250|UniProtKB:P69771}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P69771}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane; Peripheral membrane protein.
CC Endomembrane system {ECO:0000250}; Peripheral membrane protein
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000255}.
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DR EMBL; CU329671; CAA18665.2; -; Genomic_DNA.
DR PIR; T39413; T39413.
DR RefSeq; NP_596562.2; NM_001022483.2.
DR AlphaFoldDB; O60074; -.
DR SMR; O60074; -.
DR BioGRID; 276225; 6.
DR STRING; 4896.SPBC13G1.12.1; -.
DR MaxQB; O60074; -.
DR PaxDb; O60074; -.
DR EnsemblFungi; SPBC13G1.12.1; SPBC13G1.12.1:pep; SPBC13G1.12.
DR GeneID; 2539670; -.
DR KEGG; spo:SPBC13G1.12; -.
DR PomBase; SPBC13G1.12; did2.
DR VEuPathDB; FungiDB:SPBC13G1.12; -.
DR eggNOG; KOG3232; Eukaryota.
DR HOGENOM; CLU_080826_0_0_1; -.
DR InParanoid; O60074; -.
DR OMA; VMDRFET; -.
DR PRO; PR:O60074; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000815; C:ESCRT III complex; ISM:PomBase.
DR GO; GO:0005771; C:multivesicular body; IBA:GO_Central.
DR GO; GO:0032509; P:endosome transport via multivesicular body sorting pathway; IBA:GO_Central.
DR GO; GO:0045324; P:late endosome to vacuole transport; IBA:GO_Central.
DR GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; ISO:PomBase.
DR GO; GO:0006623; P:protein targeting to vacuole; IC:PomBase.
DR GO; GO:0015031; P:protein transport; IBA:GO_Central.
DR GO; GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IC:PomBase.
DR InterPro; IPR005024; Snf7_fam.
DR PANTHER; PTHR10476; PTHR10476; 1.
DR Pfam; PF03357; Snf7; 1.
PE 3: Inferred from homology;
KW Endosome; Membrane; Protein transport; Reference proteome; Transport.
FT CHAIN 1..200
FT /note="Vacuolar protein-sorting-associated protein 46"
FT /id="PRO_0000315899"
SQ SEQUENCE 200 AA; 22000 MW; 950D1FF300A78DC0 CRC64;
MSNLEASLFQ LKFAAKSLNK QSLKAAKEER AEREKVKKAI TKGNSEIARI YASNAIRKQQ
ESLNLLKLSS RIDAVSSRLQ TAVTMRAVSG NMAGVVRGMD RAMKTMNLEM ISQVMDKFEA
QFDDVNVQTG YMNKAMGSVT AVDTPQEDVD LLMQTVADEA GLEFNQNMNN NLSVPAASVP
TPAAPVEDDN LQERLRALRS
