ID   DIDA_AGKPL              Reviewed;         111 AA.
AC   C9E1S2;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Disintegrin subunit alpha;
DE   Flags: Precursor;
OS   Agkistrodon piscivorus leucostoma (Western cottonmouth) (Acontias
OS   leucostoma).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Agkistrodon.
OX   NCBI_TaxID=459671;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=18502463; DOI=10.1016/j.toxicon.2008.03.028;
RA   Jia Y., Cantu B.A., Sanchez E.E., Perez J.C.;
RT   "Complementary DNA sequencing and identification of mRNAs from the venomous
RT   gland of Agkistrodon piscivorus leucostoma.";
RL   Toxicon 51:1457-1466(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=19799929; DOI=10.1016/j.toxicon.2009.09.016;
RA   Jia Y., Perez J.C.;
RT   "Molecular cloning and characterization of cDNAs encoding
RT   metalloproteinases from snake venom glands.";
RL   Toxicon 55:462-469(2010).
CC   -!- FUNCTION: Acts by binding to alpha-IIb/beta-3 (ITGA2B/ITGB3) on the
CC       platelet surface and inhibits both ADP-induced platelet aggregation and
CC       platelet aggregate dissociation in human platelet-rich plasma.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with subunit beta; disulfide-linked.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- SIMILARITY: Belongs to the disintegrin family. Dimeric disintegrin
CC       subfamily. {ECO:0000305}.
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DR   EMBL; EV854797; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; GQ451443; ACV83937.1; -; mRNA.
DR   AlphaFoldDB; C9E1S2; -.
DR   SMR; C9E1S2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.70.10; -; 1.
DR   InterPro; IPR018358; Disintegrin_CS.
DR   InterPro; IPR001762; Disintegrin_dom.
DR   InterPro; IPR036436; Disintegrin_dom_sf.
DR   Pfam; PF00200; Disintegrin; 1.
DR   PRINTS; PR00289; DISINTEGRIN.
DR   SMART; SM00050; DISIN; 1.
DR   SUPFAM; SSF57552; SSF57552; 1.
DR   PROSITE; PS00427; DISINTEGRIN_1; 1.
DR   PROSITE; PS50214; DISINTEGRIN_2; 1.
PE   2: Evidence at transcript level;
KW   Cell adhesion impairing toxin; Disulfide bond; Secreted; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..44
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000407743"
FT   CHAIN           45..109
FT                   /note="Disintegrin subunit alpha"
FT                   /id="PRO_0000407744"
FT   PROPEP          110..111
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000407745"
FT   DOMAIN          45..111
FT                   /note="Disintegrin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT   MOTIF           89..91
FT                   /note="Cell attachment site"
FT   DISULFID        53..76
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT   DISULFID        54
FT                   /note="Interchain"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT   DISULFID        59
FT                   /note="Interchain"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT   DISULFID        67..73
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT   DISULFID        72..97
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT   DISULFID        85..104
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT   CONFLICT        65
FT                   /note="A -> S (in Ref. 1; EV854797)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   111 AA;  12103 MW;  47358809BCE06F80 CRC64;
     MIQVLLVTIC LAVFPYQGSS IILESGNVND YEVVYPRKIT PLPKGAVQPK NPCCDAATCK
     LTPGAQCAEG LCCDQCKFIK AGKICRRARG DNPDYRCTGQ SGDCPRKHFY A