DIGH_ECO57
ID DIGH_ECO57 Reviewed; 439 AA.
AC P64427; P76130;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Glycosyl hydrolase DigH {ECO:0000250|UniProtKB:P64426};
DE EC=3.2.1.- {ECO:0000250|UniProtKB:P64426};
DE AltName: Full=Divisome-localized glycosyl hydrolase {ECO:0000250|UniProtKB:P64426};
DE Flags: Precursor;
GN Name=digH {ECO:0000250|UniProtKB:P64426}; Synonyms=yddW;
GN OrderedLocusNames=Z2217, ECs2096;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Divisome-localized glycosyl hydrolase that cleaves peptide-
CC free (denuded) peptidoglycans. {ECO:0000250|UniProtKB:P64426}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000250|UniProtKB:P64426}; Lipid-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00303}. Note=Localizes to the divisome.
CC {ECO:0000250|UniProtKB:P64426}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase-like 10 (GHL10) family.
CC {ECO:0000305}.
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DR EMBL; AE005174; AAG56277.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB35519.1; -; Genomic_DNA.
DR PIR; H90890; H90890.
DR RefSeq; NP_310123.1; NC_002695.1.
DR RefSeq; WP_000350395.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P64427; -.
DR SMR; P64427; -.
DR STRING; 155864.EDL933_2149; -.
DR EnsemblBacteria; AAG56277; AAG56277; Z2217.
DR EnsemblBacteria; BAB35519; BAB35519; ECs_2096.
DR GeneID; 58391399; -.
DR GeneID; 917296; -.
DR KEGG; ece:Z2217; -.
DR KEGG; ecs:ECs_2096; -.
DR PATRIC; fig|386585.9.peg.2201; -.
DR eggNOG; COG1649; Bacteria.
DR HOGENOM; CLU_019247_0_1_6; -.
DR OMA; YGPWSEY; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR003790; GHL10.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF02638; GHL10; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Cell wall biogenesis/degradation; Glycosidase;
KW Hydrolase; Lipoprotein; Membrane; Palmitate; Reference proteome; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 28..439
FT /note="Glycosyl hydrolase DigH"
FT /id="PRO_0000013768"
FT REGION 34..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 28
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 28
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 439 AA; 49574 MW; 3E74454C52F4F0F6 CRC64;
MDICSRNKKL TIRRPAILVA LALLLCSCKS TPPESMVTPP AGSKPPATTQ QSSQPMRGIW
LATVSRLDWP PVSSVNISNP TSRARVQQQA MIDKLDHLQR LGINTVFFQV KPDGTALWPS
KILPWSDLMT GKIGENPGYD PLQFMLDEAH KRGMKVHAWF NPYRVSVNTK PGTIRELNST
LSQQPASVYV QHRDWIRTSG DRFVLDPGIP EVQDWITSIV AEVVSRYPVD GVQFDDYFYT
ESPGSRLNDN ETYRKYGGAF ASKADWRRNN TQQLIAKVSH TIKSIKPGVE FGVSPAGVWR
NRSHDPLGSD TRGAAAYDES YADTRRWVEQ GLLDYIAPQI YWPFSRSAAR YDVLAKWWAD
VVKPTRTRLY IGIAFYKVGE PSKIEPDWMI NGGVPELKKQ LDLNDAVPEI SGTILFREDY
LNKPQTQQAV SYLQSRWGS
