DIGH_SHIFL
ID DIGH_SHIFL Reviewed; 439 AA.
AC P64428; P76130;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Glycosyl hydrolase DigH {ECO:0000250|UniProtKB:P64426};
DE EC=3.2.1.- {ECO:0000250|UniProtKB:P64426};
DE AltName: Full=Divisome-localized glycosyl hydrolase {ECO:0000250|UniProtKB:P64426};
DE Flags: Precursor;
GN Name=digH {ECO:0000250|UniProtKB:P64426}; Synonyms=yddW;
GN OrderedLocusNames=SF1736, S1869;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Divisome-localized glycosyl hydrolase that cleaves peptide-
CC free (denuded) peptidoglycans. {ECO:0000250|UniProtKB:P64426}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000250|UniProtKB:P64426}; Lipid-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00303}. Note=Localizes to the divisome.
CC {ECO:0000250|UniProtKB:P64426}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase-like 10 (GHL10) family.
CC {ECO:0000305}.
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DR EMBL; AE005674; AAN43311.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP17198.1; -; Genomic_DNA.
DR RefSeq; NP_707604.1; NC_004337.2.
DR RefSeq; WP_000350395.1; NZ_WPGW01000136.1.
DR AlphaFoldDB; P64428; -.
DR SMR; P64428; -.
DR EnsemblBacteria; AAN43311; AAN43311; SF1736.
DR EnsemblBacteria; AAP17198; AAP17198; S1869.
DR GeneID; 1024952; -.
DR GeneID; 58391399; -.
DR KEGG; sfl:SF1736; -.
DR KEGG; sfx:S1869; -.
DR PATRIC; fig|198214.7.peg.2057; -.
DR HOGENOM; CLU_019247_0_1_6; -.
DR OMA; YGPWSEY; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR003790; GHL10.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF02638; GHL10; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Cell wall biogenesis/degradation; Glycosidase;
KW Hydrolase; Lipoprotein; Membrane; Palmitate; Reference proteome; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 28..439
FT /note="Glycosyl hydrolase DigH"
FT /id="PRO_0000013770"
FT REGION 34..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 28
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 28
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 439 AA; 49574 MW; 3E74454C52F4F0F6 CRC64;
MDICSRNKKL TIRRPAILVA LALLLCSCKS TPPESMVTPP AGSKPPATTQ QSSQPMRGIW
LATVSRLDWP PVSSVNISNP TSRARVQQQA MIDKLDHLQR LGINTVFFQV KPDGTALWPS
KILPWSDLMT GKIGENPGYD PLQFMLDEAH KRGMKVHAWF NPYRVSVNTK PGTIRELNST
LSQQPASVYV QHRDWIRTSG DRFVLDPGIP EVQDWITSIV AEVVSRYPVD GVQFDDYFYT
ESPGSRLNDN ETYRKYGGAF ASKADWRRNN TQQLIAKVSH TIKSIKPGVE FGVSPAGVWR
NRSHDPLGSD TRGAAAYDES YADTRRWVEQ GLLDYIAPQI YWPFSRSAAR YDVLAKWWAD
VVKPTRTRLY IGIAFYKVGE PSKIEPDWMI NGGVPELKKQ LDLNDAVPEI SGTILFREDY
LNKPQTQQAV SYLQSRWGS
