ID   DIH44_CAMFO             Reviewed;         169 AA.
AC   E2A6P1;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Diuretic hormone 44 {ECO:0000303|PubMed:25641051};
DE            Short=DH(44) {ECO:0000303|PubMed:25641051};
DE   Flags: Precursor;
GN   ORFNames=EAG_05630 {ECO:0000312|EMBL:EFN70881.1};
OS   Camponotus floridanus (Florida carpenter ant).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Formicinae; Camponotus.
OX   NCBI_TaxID=104421;
RN   [1] {ECO:0000312|EMBL:EFN70881.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=20798317; DOI=10.1126/science.1192428;
RA   Bonasio R., Zhang G., Ye C., Mutti N.S., Fang X., Qin N., Donahue G.,
RA   Yang P., Li Q., Li C., Zhang P., Huang Z., Berger S.L., Reinberg D.,
RA   Wang J., Liebig J.;
RT   "Genomic comparison of the ants Camponotus floridanus and Harpegnathos
RT   saltator.";
RL   Science 329:1068-1071(2010).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 97-108, TISSUE SPECIFICITY, MASS SPECTROMETRY,
RP   IDENTIFICATION BY MASS SPECTROMETRY, AMIDATION AT ILE-108, AND
RP   PYROGLUTAMATE FORMATION AT GLN-97.
RX   PubMed=25641051; DOI=10.1021/pr5011636;
RA   Schmitt F., Vanselow J.T., Schlosser A., Kahnt J., Roessler W., Wegener C.;
RT   "Neuropeptidomics of the carpenter ant Camponotus floridanus.";
RL   J. Proteome Res. 14:1504-1514(2015).
CC   -!- FUNCTION: Regulation of fluid secretion.
CC       {ECO:0000250|UniProtKB:P82707}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:25641051}.
CC   -!- TISSUE SPECIFICITY: Expressed in brain, ventral ganglia and the
CC       retrocerebral complex (at protein level).
CC       {ECO:0000269|PubMed:25641051}.
CC   -!- PTM: Residues Ile-66 to Gly-109 may constitute another form of the DH44
CC       peptide, which has not been detected yet.
CC       {ECO:0000303|PubMed:25641051}.
CC   -!- MASS SPECTROMETRY: Mass=1542.80; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:25641051};
CC   -!- SIMILARITY: Belongs to the sauvagine/corticotropin-releasing
CC       factor/urotensin I family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; GL437183; EFN70881.1; -; Genomic_DNA.
DR   AlphaFoldDB; E2A6P1; -.
DR   STRING; 104421.E2A6P1; -.
DR   InParanoid; E2A6P1; -.
DR   OMA; RERQDDQ; -.
DR   Proteomes; UP000000311; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   InterPro; IPR000187; CRF.
DR   Pfam; PF00473; CRF; 1.
DR   SMART; SM00039; CRF; 1.
PE   1: Evidence at protein level;
KW   Amidation; Coiled coil; Direct protein sequencing; Hormone;
KW   Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   PROPEP          19..96
FT                   /evidence="ECO:0000305|PubMed:25641051"
FT                   /id="PRO_0000434206"
FT   PEPTIDE         97..108
FT                   /note="Diuretic hormone 44"
FT                   /evidence="ECO:0000269|PubMed:25641051"
FT                   /id="PRO_0000434207"
FT   PROPEP          109..169
FT                   /evidence="ECO:0000305|PubMed:25641051"
FT                   /id="PRO_0000434208"
FT   COILED          83..108
FT                   /evidence="ECO:0000255"
FT   MOD_RES         97
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:25641051"
FT   MOD_RES         108
FT                   /note="Isoleucine amide"
FT                   /evidence="ECO:0000269|PubMed:25641051"
SQ   SEQUENCE   169 AA;  19600 MW;  1B08867BEE416C61 CRC64;
     MILLGILAST TIIGLTSSAP LSSYERRDVS DNRPKIFLLM DERIPELENE ILGNELGSDV
     TRTKRIGSLS IVNNLDVLRN RVMLELARRK QERDQRQIEE NRRFLENIGK RSVPVSDAGK
     IVRSDKSRND RNQPLQFNRI EWIEEEDPLF RGSQDDQMTR VQANELRLL