DIK2A_MOUSE
ID DIK2A_MOUSE Reviewed; 430 AA.
AC Q3USZ8; E9Q9E9;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Divergent protein kinase domain 2A {ECO:0000305};
DE AltName: Full=Deleted in autism protein 1 homolog;
DE AltName: Full=Golgi Protein of 49 kDa;
DE Short=GoPro49;
DE AltName: Full=Hypoxia and AKT-induced stem cell factor;
DE Short=HASF;
DE Flags: Precursor;
GN Name=Dipk2a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=18651652; DOI=10.1002/dvdy.21646;
RA Takatalo M., Jaervinen E., Laitinen S., Thesleff I., Roennholm R.;
RT "Expression of the novel Golgi protein GoPro49 is developmentally regulated
RT during mesenchymal differentiation.";
RL Dev. Dyn. 237:2243-2255(2008).
RN [4]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=19587158; DOI=10.1177/0022034509338452;
RA Takatalo M.S., Tummers M., Thesleff I., Roennholm R.;
RT "Novel Golgi protein, GoPro49, is a specific dental follicle marker.";
RL J. Dent. Res. 88:534-538(2009).
RN [5]
RP FUNCTION IN CARDIOMYOCYTE PROLIFERATION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=23784961; DOI=10.1161/circresaha.113.301075;
RA Beigi F., Schmeckpeper J., Pow-Anpongkul P., Payne J.A., Zhang L.,
RA Zhang Z., Huang J., Mirotsou M., Dzau V.J.;
RT "C3orf58, a novel paracrine protein, stimulates cardiomyocyte cell-cycle
RT progression through the PI3K-AKT-CDK7 pathway.";
RL Circ. Res. 113:372-380(2013).
CC -!- FUNCTION: May play a role in cardiomyocyte proliferation through
CC paracrine signaling and activation of the PI3-kinase signaling cascade.
CC {ECO:0000269|PubMed:23784961}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle, COPI-coated
CC vesicle. Secreted.
CC -!- TISSUE SPECIFICITY: Expressed in heart, brain, liver, spleen, kidney,
CC lung, thymus, testis, ovary and muscle. {ECO:0000269|PubMed:23784961}.
CC -!- DEVELOPMENTAL STAGE: As early as 12.5 dpc to 16.5 dpc, intense
CC expression is restricted to mesenchymal condensations, which form the
CC different cartilage elements of the developing skeleton. At 12.5 dpc,
CC strongly expressed in all vertebrae, heart ventricle, dental follicle,
CC interdigital tissue, tongue and cartilages of the basioccipital bone,
CC maxilla, mandible and the region of the future nasal septum. At 14.5
CC and 16.5 dpc, expressed in ribs, long bones, interdigital tissue,
CC metatarsals, dental follicle, cartilages of the mandible and nasal
CC septum as well as vertebrae with stronger expression in posterior
CC vertebrae. At 16.5 dpc, also expressed in paw bones and, in vertebrae,
CC the expression is limited to mainly columnar, proliferating
CC chondrocytes. At 4 days postnatal, intense expression in nasal
CC cartilages, hair, dental and vibrissa follicles.
CC {ECO:0000269|PubMed:18651652, ECO:0000269|PubMed:19587158}.
CC -!- SIMILARITY: Belongs to the DIPK family. {ECO:0000305}.
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DR EMBL; AK139920; BAE24182.1; -; mRNA.
DR EMBL; AC160129; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS40725.1; -.
DR RefSeq; NP_001028317.2; NM_001033145.2.
DR AlphaFoldDB; Q3USZ8; -.
DR STRING; 10090.ENSMUSP00000108651; -.
DR PhosphoSitePlus; Q3USZ8; -.
DR SwissPalm; Q3USZ8; -.
DR EPD; Q3USZ8; -.
DR MaxQB; Q3USZ8; -.
DR PaxDb; Q3USZ8; -.
DR PeptideAtlas; Q3USZ8; -.
DR PRIDE; Q3USZ8; -.
DR Antibodypedia; 52825; 100 antibodies from 16 providers.
DR Ensembl; ENSMUST00000113028; ENSMUSP00000108651; ENSMUSG00000045414.
DR GeneID; 68861; -.
DR KEGG; mmu:68861; -.
DR UCSC; uc009rax.1; mouse.
DR CTD; 205428; -.
DR MGI; MGI:1916111; Dipk2a.
DR VEuPathDB; HostDB:ENSMUSG00000045414; -.
DR eggNOG; ENOG502QUEB; Eukaryota.
DR GeneTree; ENSGT00520000055625; -.
DR HOGENOM; CLU_052524_0_0_1; -.
DR InParanoid; Q3USZ8; -.
DR OMA; TKICRRA; -.
DR OrthoDB; 1199714at2759; -.
DR PhylomeDB; Q3USZ8; -.
DR TreeFam; TF313319; -.
DR BioGRID-ORCS; 68861; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q3USZ8; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q3USZ8; protein.
DR Bgee; ENSMUSG00000045414; Expressed in cerebellum lobe and 290 other tissues.
DR Genevisible; Q3USZ8; MM.
DR GO; GO:0030126; C:COPI vesicle coat; ISO:MGI.
DR GO; GO:0030137; C:COPI-coated vesicle; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; IDA:MGI.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; ISO:MGI.
DR GO; GO:0060038; P:cardiac muscle cell proliferation; ISS:UniProtKB.
DR GO; GO:0034392; P:negative regulation of smooth muscle cell apoptotic process; IMP:MGI.
DR GO; GO:1900020; P:positive regulation of protein kinase C activity; ISO:MGI.
DR GO; GO:0014066; P:regulation of phosphatidylinositol 3-kinase signaling; ISS:UniProtKB.
DR GO; GO:0048199; P:vesicle targeting, to, from or within Golgi; IC:MGI.
DR InterPro; IPR020519; DIPK2A/B.
DR InterPro; IPR022049; FAM69_kinase_dom.
DR PANTHER; PTHR32073; PTHR32073; 1.
DR Pfam; PF12260; PIP49_C; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Golgi apparatus; Reference proteome; Secreted; Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..430
FT /note="Divergent protein kinase domain 2A"
FT /id="PRO_0000226038"
FT CONFLICT 3..22
FT /note="RLVPLKLGRLSRALKLAALG -> MAVVLRMSNI (in Ref. 1;
FT BAE24182)"
FT /evidence="ECO:0000305"
FT CONFLICT 426..430
FT /note="SHNVR -> KSQCEVVSSDLYIFFAEKALTKLNHQKNCLE (in Ref. 1;
FT BAE24182)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 430 AA; 49463 MW; 4A63FFC4E13379D0 CRC64;
MWRLVPLKLG RLSRALKLAA LGSLLVLMLL HSPSLLASWQ RNELADRRFL QLNKCPACFG
TSWCRRFLNG QVGFETWGRL RLLDFLNVKN VYFAQYGEPR EGGRRRVVLK RLGSQRELAQ
LDQSICKRAT GRPRCDLLQA MPRTEFARLN GDVRLLTPEA VEGWSDLVHC PSQRLLDRLV
RRYAETKDSG SFLLRNLKDS ERMQLLLTLA FNPEPLVLQS FPSDEGWPFA KYLGACGRMV
AVNYVGEELW SYFNAPWEKR VDLAWQLMEI AEQLTNNDFE FALYLLDVSF DNFAVGPRDG
KVIIVDAENV LVADKRLIRQ NKPENWDVWY ESKFDDCDKE ACLSFSKEIL CARVTVDHNY
YAVCQNLLSR HATWRGTSGG LLHDPPSEIA KDGRLEALLD ECTNPKKRYG RFQAAKELRG
YLAQLSHNVR
